Molecular parasite diagnosis has undergone a significant transformation, evolving from traditional methods such as direct observation and enrichment techniques followed by microscopic examination to more sophisticated approaches involving the detection of antibodies, antigens, and nucleic acids. The current problem in the field is the need for more sensitive, specific, and rapid diagnostic methods that can be applied in diverse settings, including those with limited resources. Recent studies have demonstrated the high sensitivity and specificity of molecular techniques in detecting parasite nucleic acids from various sample types. These methods can be both quantitative and qualitative, and visualization can be achieved using advanced equipment or even through direct observation. Despite these advancements, there are still gaps in the implementation of these techniques in field settings, particularly in resource-limited environments. The integration of Point-of-Care Tests (POCT) has shown promise in providing rapid and accurate diagnostics, but there is a need for further research to address issues of feasibility, sustainability, and standardization.

This research topic aims to explore the use of molecular techniques in the diagnosis of parasites, with a particular focus on their application within the One Health framework. The main objectives include evaluating the feasibility and sustainability of these techniques compared to gold-standard methods and examining their use in the surveillance of zoonotic parasites within control programs. Additionally, the research will aim to develop, standardize, and adapt molecular techniques for practical use in diverse settings.

To gather further insights into the boundaries of molecular parasite diagnosis, we welcome articles addressing, but not limited to, the following themes:
- Feasibility and sustainability of molecular techniques compared to traditional methods
- Application of molecular techniques in the surveillance of zoonotic parasites
- Development and standardization of molecular diagnostic methods
- Adaptation of molecular techniques for use in resource-limited settings
- Implementation and impact of Point-of-Care Tests (POCT) in field diagnostics
- Case studies and reports on the practical use of molecular techniques in parasite diagnosis
- Comparative studies on the sensitivity and specificity of different molecular diagnostic methods

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Frontiers in Parasitology

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