This Research Topic is the second volume of the “Methods in Cancer Immunity and Immunotherapy: 2022”. Please see Volume I here. The Research Topic is also part of the “Methods in Immunology” series.

This series aims to highlight the latest experimental techniques and methods used to investigate fundamental questions in Immunology research, with a focus on Cancer Immunity and Immunotherapy. Review articles or opinions on methodologies or applications including the advantages and limitations of each are welcome. This Topic includes technologies and up-to-date methods which help advance science.

The contributions to this collection will undergo peer review. Novelty may vary, but the utility of a method or protocol must be evident. We welcome contributions covering all methodological aspects regarding Cancer Immunity and Immunotherapy. Submissions will be handled by the team of Topic Editors in the respective sections.

This Research Topic welcomes:

• Methods: Describing either new or existing methods that are significantly improved or adapted for specific purposes. These manuscripts may include primary (original) data.

• Protocols: Detailed descriptions, including pitfalls and troubleshooting, to benefit those who may evaluate or employ the techniques. The protocols must be proven to work.

• Perspective or General Commentaries on methods and protocols relevant for physiology research.

• Reviews and mini-reviews of topical methods and protocols highlighting the important future directions of the field.

Manuscripts consisting solely of bioinformatics or computational analysis of public genomic or transcriptomic databases which are not accompanied by robust and relevant validation (clinical cohort or biological validation in vitro or in vivo) are out of scope for this topic.

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