In vitro plant tissue culture is a burgeoning field that offers promising solutions for the sustainable production of natural bioactive compounds. Historically, humans have relied on plants for nourishment and healing, utilizing the myriad of metabolites they synthesize, such as alkaloids, terpenoids, flavonoids, and phenolics, which possess antioxidative, antimicrobial, and anti-inflammatory properties. The demand for these secondary metabolites has surged across various industries, including pharmaceuticals, cosmetics, agrochemicals, and food additives. However, the traditional extraction of these compounds faces significant challenges due to seasonal dependencies and limited yields. Plant tissue culture emerges as a viable alternative, providing a controlled environment for continuous and standardized production. Despite these advancements, there remains a critical need to explore and optimize these biotechnological methods further, addressing the environmental and biodiversity concerns associated with traditional extraction methods.

This research topic aims to gather publications focused on evaluating and optimizing biosynthetic pathways leading to the production of bioactive compounds in in vitro grown plant material. The objective is to establish in vitro-derived plant material as a sustainable and viable source of secondary metabolites. Additionally, the research seeks to identify and develop green extraction techniques that minimize environmental impact. Furthermore, the topic invites studies on scaling up production processes and enhancing the cost-effectiveness of tissue culture methods, ensuring that these biotechnological advancements can be practically applied on a larger scale.

To gather further insights in the sustainable production of bioactive compounds through plant tissue culture, we welcome articles addressing, but not limited to, the following themes:
- Overview of tissue culture techniques and their potential for producing natural bioactive compounds such as alkaloids, terpenoids, flavonoids, and phenolics.
- Analysis of the properties of bioactive compounds produced by tissue culture and their potential for use in pharmaceutical, cosmetic, and agri-food industries.
- Comparative analysis of the nature of bioactive compounds extracted from vitro-derived plant material and in vivo grown plants, including yield, quality, sustainability, and cost-effectiveness.
- Individuating alternative green extraction methods for the production of natural bioactive compounds.
- Advantages and limitations of tissue culture for natural bioactive compound production.
- Environmental and ethical considerations related to tissue culture, including conservation of endangered plant species and reduction of biodiversity impoverishment.
- Economic and commercial feasibility of tissue culture for bioactive compound production, including scalability and cost-effectiveness.

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Frontiers in Natural Products

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