Complete elimination of gluten from the diet is currently the only accepted treatment for celiac disease. Celiac disease patients are principally dependent on gluten-free food products commercially available on the market. In recent years, a substantial amount of gluten contamination in gluten-free food and ...
Complete elimination of gluten from the diet is currently the only accepted treatment for celiac disease. Celiac disease patients are principally dependent on gluten-free food products commercially available on the market. In recent years, a substantial amount of gluten contamination in gluten-free food and gluten-free food products has been reported. Unintended gluten transgression in more than the accepted amount of gluten (>20 mg/kg) on daily basis can damage the small intestinal integrity. Several antibodies (R5, G12, α-20, MIoBS, DQ2.5-glia-α3) have been developed to quantify gluten. Among these, R5 is the most prevalent antibody and official ELISA method for gluten detection. It strongly recognizes the toxic fragments of α-gliadin (QQPFP, QQQFP, LQPFP, and QLPFP) sequences and is widely used for gluten analysis in food matrices. G12 antibody, developed against the α2-gliadin 33-mer toxic peptide of the gliadin, is another frequently used antibody for gluten detection. While the G12 antibody-based ELISA method is used to quantify gluten in the food matrices. G12 antibody-based lateral flow test is used to quantify gluten traces in the urine and stool samples. Though these methods efficiently track gluten, they have certain limitations. These methods do not accurately quantify gluten traces and often show significant discrepancies in the gluten estimation results. Recently introduced 13F6 and 14G11 antibodies-based portable gluten sensors offer instant gluten detection in food samples. Nevertheless, there is variation in the results. Such sensors certainly require further validation. On the other hand, Liquid Chromatography Mass Spectrometry (LC-MS/MS), a proteomics- based analytical technique, accurately quantifies the minute amount of gluten and is used for gluten proteome profile. LC-MS/MS is endorsed as the most important alternative method to detect gluten, especially in fermented foods. Conversely, LC-MS/MS is a highly sophisticated, expensive, and complex process to perform in general lab settings. Hence, it cannot be established as a routine gluten analytical method. Despite these various gluten detection tools, gluten contamination remains a significant issue. There is certainly a need to be updated about current gluten detection approaches along with their limitations in order to develop a reliable and easy-to-perform gluten tracking method that can precisely determine the gluten content in foodstuff and/or clinical samples.
This Research Topic aims to provide comprehensive information about current approaches to accurately detect and quantify gluten in food products. The topic will also cover the possibilities of novel evolving gluten detection methods. Subtopics of interest include (but are not limited to):
• Gluten contamination in gluten-free products
• Novel methods to detect gluten quantity
• Gluten quantification in clinical samples (e.g., stool and urine)
• Gluten contamination in gluten-free restaurants/food industries
• Home-based devices to detect gluten
You can find Volume I of this impactful Research Topic here.
Keywords:
gluten, contamination
Important Note:
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