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ORIGINAL RESEARCH article

Front. Sustain. Food Syst.
Sec. Nutrition and Sustainable Diets
Volume 9 - 2025 | doi: 10.3389/fsufs.2025.1520875
This article is part of the Research Topic Mushrooms as Functional Foods and Nutraceuticals View all 5 articles

Stimulation of Glucose Uptake by Edible Pholiota nameko (T.Itô) S.Ito & S.Imai Extract Counteracts Palmitate-Induced Insulin Resistance in C2C12 Myotubes

Provisionally accepted
Sing-Chung Li Sing-Chung Li 1*Chiao-Ming Chen Chiao-Ming Chen 2Seng-Kai Vong Seng-Kai Vong 1Arpita Das Arpita Das 1Lie-Fen Shyur Lie-Fen Shyur 3Yu-Ming Ju Yu-Ming Ju 4Huei-Mei Hsieh Huei-Mei Hsieh 4Shu-Hui Yang Shu-Hui Yang 5Viola Varga Viola Varga 6
  • 1 School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, Taiwan
  • 2 Department of Food Science, Nutrition and Nutraceutical Biotechnology, College of Human Ecology, Shih Chien University, Taipei, Taiwan
  • 3 Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan
  • 4 Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan
  • 5 Fengshan Tropical Horticultural Experiment Branch, Taiwan Agricultural Research Institute, Kaohsiung, Taiwan
  • 6 Centre for Translational Medicine, Semmelweis University, Budapest, Hungary

The final, formatted version of the article will be published soon.

    Pholiota nameko (P. nameko) was identified as an edible mushroom known for its abundance of bioactive compounds. Previous studies suggested that extracts from this mushroom possess antioxidant properties attributed to the presence of phenolic acids, such as 4-hydroxybenzoic acid (4-HBA) and cinnamic acid (CA). These compounds show promise in potentially ameliorating insulin resistance. In this study, the highest levels of 4-HBA and CA were selected from six P. nameko extracts using ultra-high-pressure liquid chromatography (UHPLC) to investigate whether they could improve the glucose uptake resistance induced by palmitate in C2C12 myotubes. Additionally, the protein expression of GLUT4 was examined. The results indicated that P. nameko hot water extract (PN-HW) exhibited the highest total polyphenol content (9.34 ± 0.31 mg GAE/g) and demonstrates strong free radical scavenging ability (77.4 ± 1.1%). Furthermore, PN-HW contains notable amounts of 4-HBA (4.02 ± 0.17 mg/g DW) and CA (2.01 ± 0.09 mg/g DW). Importantly, the cell viability reveals that PN-HW extracts did not have a toxic effect on C2C12 myotubes. PN-HW significantly enhances glucose uptake at doses of 25 μg/mL. Hence, it was suggested that PN-HW might contain elevated levels of phenolic acids, potentially mitigating impaired glucose uptake caused by high-fat content and consequently enhancing the glucose uptake capacity of C2C12 cells.

    Keywords: Pholiota nameko water extract, Insulin Resistance, phenolic acids, cinnamic acid, 4-Hydroxybenzoic acid

    Received: 31 Oct 2024; Accepted: 30 Jan 2025.

    Copyright: © 2025 Li, Chen, Vong, Das, Shyur, Ju, Hsieh, Yang and Varga. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Sing-Chung Li, School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, Taiwan

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