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ORIGINAL RESEARCH article

Front. Sustain. Food Syst.

Sec. Nutrition and Sustainable Diets

Volume 9 - 2025 | doi: 10.3389/fsufs.2025.1474848

Phytochemical Profiling of Tagetes Erecta L. Flowers at Various Blooming Stages Through Optimized Extraction of Bioactive Compounds for the Development of Functional Juice

Provisionally accepted
  • 1 The University of Melbourne, Parkville, Victoria, Australia
  • 2 Khwaja Fareed University of Engineering and Information Technology (KFUEIT), Rahim Yar Khan, Punjab, Pakistan
  • 3 University of Castilla-La Mancha, Ciudad Real, Castilla-La Mancha, Spain
  • 4 Guangdong Ocean University, Zhanjiang, China
  • 5 King Saud University, Riyadh, Riyadh, Saudi Arabia

The final, formatted version of the article will be published soon.

    Tagetes erecta L. is a valuable medicinal plant and its edible flowers are a rich source of secondary metabolites that may vary at different blooming stages. The current study aimed to evaluate the phytochemical profile of flowers procured at different stages of blooming from the start of flowering to the end of the season and their antioxidant activities by modifying conventional solvent extraction methods with ultrasound pretreatment and by varying solvents (hexane, ethanol, acetone). The results showed that ultrasound-pretreated samples yielded higher percentages than solvent extraction alone. Functional metabolites were also found to be higher in pre-treated samples at various stages and polar solvents. The highest carotenoid content (427.39 mg/100 g) was observed for the pretreated full-bloom flower samples (MAF-1). Radical scavenging activities were observed highest (DPPH 96.19% and ABTS+ 160.09 mmol TE/100 g) in full-bloom marigold flower samples MAF-1 followed by MEF-1 and MAI-1. A highly significant and positive correlation was observed among all variables of phenolic and antioxidant assays. Radical scavenging activities of samples were positively correlated with carotenoid content, flavonoids, and phenolics. Various bioactive compounds including phenolic acids, flavonoids and carotenoids were quantified by HPLC-DAD. Results showed that the ultrasound acetone extracts of marigold (MAF-1) resulted in higher gallic acid (109 mg/g), quercetin (11.74 mg/g), lutein (1.76 mg/g) and zeaxanthin (2.14 mg/g) followed by MEF-1 > MAI-1>MEI-1. The study results showed that the ultrasound assisted extraction of full-bloom flowers (peak season) resulted in higher bioactive contents. Functional juice was prepared by supplementing these purified extracts in various concentrations (0-10 mg) to juice, which resulted in higher acceptability due to improved sensory characteristics. Study results evident that the marigold bioactive compounds hold good potential for the development of functional foods aiming to control early age-related disorders (ARDs).

    Keywords: Tagetes, Ultrasonification, polyphenolics, Carotenoids, HPLC

    Received: 02 Aug 2024; Accepted: 28 Mar 2025.

    Copyright: © 2025 Siddiqa, Khaliq, Mehmood, Chughtai, Sanchez-Migallon, Ahsan, Sabir and Ahmed. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Ayesha Siddiqa, The University of Melbourne, Parkville, 3010, Victoria, Australia
    Amanullah Sabir, Guangdong Ocean University, Zhanjiang, China
    Isam A. Mohamed Ahmed, King Saud University, Riyadh, 11451, Riyadh, Saudi Arabia

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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