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ORIGINAL RESEARCH article
Front. Sustain. Food Syst.
Sec. Agro-Food Safety
Volume 8 - 2024 |
doi: 10.3389/fsufs.2024.1490750
This article is part of the Research Topic Rapid Screening for Organic Pollutants Analysis in Food View all 9 articles
A hapten design strategy to enhance the selectivity of monoclonal antibodies against malachite green
Provisionally accepted
Min-Fu Wu
1*
Nuo Xu
2*
Sha Li
1*
Yi-Lan Huang
2*
Wu Minhua
3
Jia-Dong Li
1*
Ri-Sheng Chen
2*
Wen-Ming Xiong
4*
Yong-Jun Li
4*
Hongtao Lei
2
Xin-An Huang
5*
Zhen-Lin Xu
2- 1 Foshan Polytechnic, Foshan, China
- 2 South China Agricultural University, Guangzhou, Guangdong Province, China
- 3 Guangdong Medical University, Zhanjiang, Guangdong, China
- 4 Guangzhou Vocational College of Technology &Business, Guangzhou, China
- 5 Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, China
Malachite green (MG), a triphenylmethane dye, is used in the aquaculture industry as a disinfectant and insect repellent due to its potent bactericidal and pesticidal properties. However, its use poses potential environmental and health risks. This study analyzed and designed two haptens using computer simulation. Serum data confirmed the feasibility of introducing an arm at the dimethylamine group. Subsequently, a highly selective monoclonal antibody strain was successfully prepared based on the hapten. After optimizing the working conditions of indirect competitive enzyme-linked immunosorbent assay (IC-ELISA), the IC50 value was 0.83 ng/mL, with a detection limit (IC10) of 0.08 ng/mL and a linear range of 0.19-3.52 ng/mL. The developed monoclonal antibody exhibited a crossover rate of less than 0.1% with other similar structures and can be used to establish an immunoassay.
Keywords: Malachite green, Monoclonal antibody, molecular simulation, ic-ELISA, Hapten design
Received: 03 Sep 2024; Accepted: 18 Oct 2024.
Copyright: © 2024 Wu, Xu, Li, Huang, Minhua, Li, Chen, Xiong, Li, Lei, Huang and Xu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Min-Fu Wu, Foshan Polytechnic, Foshan, China
Nuo Xu, South China Agricultural University, Guangzhou, 510642, Guangdong Province, China
Sha Li, Foshan Polytechnic, Foshan, China
Yi-Lan Huang, South China Agricultural University, Guangzhou, 510642, Guangdong Province, China
Jia-Dong Li, Foshan Polytechnic, Foshan, China
Ri-Sheng Chen, South China Agricultural University, Guangzhou, 510642, Guangdong Province, China
Wen-Ming Xiong, Guangzhou Vocational College of Technology &Business, Guangzhou, China
Yong-Jun Li, Guangzhou Vocational College of Technology &Business, Guangzhou, China
Xin-An Huang, Guangzhou University of Chinese Medicine, Guangzhou, 510006, Guangdong Province, China
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