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ORIGINAL RESEARCH article

Front. Sustain. Food Syst.
Sec. Nutrition and Sustainable Diets
Volume 8 - 2024 | doi: 10.3389/fsufs.2024.1487774

Protective effects of soybean peptides on H2O2-induced oxidative injury in IPEC-J2 Cells

Provisionally accepted
Fushan Lu Fushan Lu 1,2Qingqiang Yin Qingqiang Yin 1*Xiao Wang Xiao Wang 2Lijuan Xu Lijuan Xu 2Mengjie Liu Mengjie Liu 1Qian Zhang Qian Zhang 1Hai Sheng Hai Sheng 1Chaoqi Liu Chaoqi Liu 1Ping Wang Ping Wang 1*Lijun Wang Lijun Wang 1Sanjun Jin Sanjun Jin 1Xinxin Li Xinxin Li 1Juan Chang Juan Chang 1Qun Zhu Qun Zhu 3
  • 1 Henan Agricultural University, Zhengzhou, China
  • 2 Henan PUAI Feed Co., Ltd, Zhoukou, China
  • 3 Henan Delin Biological Product Co. Ltd., Zhengzhou, China

The final, formatted version of the article will be published soon.

    The purpose of the study was to demonstrate how soybean peptides(SBP)protect against H2O2 -induced injury in intestinal porcine epithelial cells (IPEC-J2). SBP were prepared by protease hydrolysis, in which the molecular weights of 95.76% SBP were smaller than 3 kDa. Cell experiment included four groups: Control group (IPEC-J2 cells were treated with HGDMEM), SBP group (100 μg/mL SBP incubation for 13 h), H2O2 treatment group (1 mM H2O2 treatment for 1 h), SBP + H2O2 group (100 μg/mL SBP pretreatment for 12 h followed by 1mM H2O2 treatment for 1 h). This study showed that that treatment with single 1 mM H2O2 for 1 h significantly reduced cell viability to 52.99% (p < 0.05), up-regulated Bax and Caspase-3 gene expressions (p < 0.05), and down-regulated gene expressions of ZO-1, CAT, SOD1, HO-1 and Nrf2 ( p < 0.05), compared with the control group. However, pretreatment with SBP followed by H2O2 inducement significantly increased cell viability to 72.99%, decreased cell apoptosis, increased SOD, CAT and GSH-Px activity (p < 0.05), down-regulated Bax and Caspase-3 gene expressions (p < 0.05), and up-regulated the gene expressions of ZO-1, Claudin-1, Occludin, catalase, glutathione GPX1, SOD1, HO-1, NQO1 and Nrf2, compared with the single H2O2-induced cells. According to the study, SBP pretreatment reduced H2O2-induced oxidative stress in cells and preserved the integrity of intestinal cells. Keywords Soybean peptides • oxidative stress • IPEC-J2 cells • Antioxidant capacity • Gene expressions

    Keywords: Soybean peptides, Oxidative Stress, IPEC-J2 cells, antioxidant capacity, gene expressions

    Received: 28 Aug 2024; Accepted: 28 Nov 2024.

    Copyright: © 2024 Lu, Yin, Wang, Xu, Liu, Zhang, Sheng, Liu, Wang, Wang, Jin, Li, Chang and Zhu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Qingqiang Yin, Henan Agricultural University, Zhengzhou, China
    Ping Wang, Henan Agricultural University, Zhengzhou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.