Skip to main content

ORIGINAL RESEARCH article

Front. Sustain. Food Syst.
Sec. Sustainable Food Processing
Volume 8 - 2024 | doi: 10.3389/fsufs.2024.1443708
This article is part of the Research Topic Fermented-Based Foods As Sustainable Alternative Sources To Meet Future Demand For Protein View all 6 articles

Unlocking hypolipidemic potential of bioactive peptides derived from probiotic fermented cattle, camel, goat and sheep milk: a comprehensive investigation through in vitro, in silico, and molecular docking studies

Provisionally accepted
Priti Mudgil Priti Mudgil 1,2*Feyisola F. Ajayi Feyisola F. Ajayi 1Aysha S. Alyafei Aysha S. Alyafei 1Pei Gee Yap Pei Gee Yap 3Chee Yuen Gan Chee Yuen Gan 3Sajid Maqsood Sajid Maqsood 1
  • 1 College of Agriculture and Veterinary Medicine, United Arab Emirates University, AlAin, United Arab Emirates
  • 2 United Arab Emirates University, Al-Ain, Abu Dhabi, United Arab Emirates
  • 3 Analytical Biochemistry Research Centre, Universiti Sains Malaysia, George Town, Penang, Malaysia

The final, formatted version of the article will be published soon.

    In this study, fermented cattle milk (FCTM), fermented camel milk (FCM), fermented goat milk (FGM), and fermented sheep milk (FSM) were produced using a total of 5 probiotic bacterial strains to investigate the release of bioactive peptides with hypolipidemic potential via in vitro inhibitory activity towards pancreatic lipase (PL) during a 14-days refrigerated storage study. The PL-inhibitory activities of these fermented milks varied according to type of probiotic strains and milk types used. Overall, Pediococcus pentosaceus MF000957 (PP-957) strain showed the highest PL inhibitory activity spanning across all milk types and therefore, PP-957 derived fermented samples were analyzed for bioactive peptide (BAP) identification by LCMS-QTOF. Identified BAP were further analyzed using in silico and bioinformatics approaches for bioactivity prediction, molecular docking and drug pharmacokinetic studies. Overall, 4 peptides derived from FCTM, 1 from FCM and 2 peptides common in FGM and FSM were predicted as active PL inhibitors based on their binding energy and number of binding sites on PL enzyme. All peptides were non-toxic, non-carcinogenic and had appropriate drug-like properties. The outcomes of this study suggest that fermented milk derived peptides from animal milks are anticipated to be useful for combating hypercholesterolemia.

    Keywords: probiotic fermentation, Pancreatic lipase, Milk Proteins, Dairy animals, Bioactive peptides

    Received: 04 Jun 2024; Accepted: 07 Aug 2024.

    Copyright: © 2024 Mudgil, Ajayi, Alyafei, Yap, Gan and Maqsood. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Priti Mudgil, College of Agriculture and Veterinary Medicine, United Arab Emirates University, AlAin, United Arab Emirates

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.