Neilton Antonio Fiusa Araújo1
Rafaela Magalhães Brandão2Beatriz Meireles Barguil3Maria das Graças Cardoso2Moacir Pasqual1Renata Alves Lara Silva Rezende1Maysa Mathias Alves Pereira1Victor Hugo Teixeira Buttrós1
Joyce Dória1*- 1Department of Agriculture, Federal University of Lavras, Lavras, Brazil
- 2Department of Chemistry, Federal University of Lavras, Lavras, Brazil
- 3Department of Biological Sciences, Federal University of Piauí, Teresina, Brazil
Rose essential oil is rich in compounds widely used by the pharmaceutical and cosmetic industry, due to the biological activities it presents. However, obtaining oil is costly, as the yield per plant is low, which requires several techniques that aim to increase its production. The application of growth-promoting bacteria has been studied for this purpose. Thus, the objective of this work was to select efficient bacteria for production and evaluate their influence on the phytotechnical characteristics and composition of the essential oils of roses. Seven species of bacteria were evaluated for the potential to promote growth in vitro, being tested for nitrogen fixation, phosphate solubilization, protease production and auxin production. From bacteria tested, four were selected and inoculated on rose plants of cultivar Black Prince to evaluate the influence on phytotechnical variables of flower and stem and the oil production. The evaluation of the production of roses was performed through the characteristics of the flowers (size, weight, and diameter of the stem) and floral bud. The essential oils from the inoculated flowers were extracted and evaluated in terms of content, yield, and chemical composition. The application of B. acidiceler, B. subtilis and B. pumilus resulted in flowers with a diameter up to 29% larger. The floral stem was increased by up to 24.5% when B. acidiceler and B. pumilus were used. Meanwhile, the stem diameter was around 41% greater in the presence of B. acidiceler, B. subtilis and in the control. Bacillus pumilus also increased the weight of fresh petals (104%) and essential oil yield (26%), changing the chemical composition of the extracted essential oil. Thus, it is concluded that B. acidiceler, B. pumilus, and B. subtilis improved the phytotechnical characteristics of roses. Among bacteria, B. pumilus increased the essential oil content as well as positively changed the chemical composition of the extracted essential oil.
Introduction
Rose (Rosa hybrida L. - Rosaceae) is one of the three main flowering plants in the world. Currently, there are approximately 35,000 cultivars bred throughout the world (Bendahmane et al., 2013; Kirov et al., 2014) and around 200 species cataloged (Fougère-Danezan et al., 2015). Roses are cultivated around the world for different purposes, from ornamentation to the manufacture of processed products such as perfumes, medicines, and food using bioactive molecules found in their flowers (Gil et al., 2019). Among these molecules, the essential oils (EO) are one of the most researched in roses. EO are secondary metabolites extracted from plants. They are commonly used in the food, cosmetic and pharmaceutical industries, due to their several biological activities, such as antimicrobial, analgesic, antiseptic, carminative, and diuretic; in many cases, EO also show pleasant diverse aromas (Calo et al., 2015; Basak and Guha, 2018; Sarkic and Stappen, 2018).
Brazil has a prominent place in the production of EO, alongside India, China and Indonesia, mainly due to the production of EO extracted from citrus fruits as by-products of the juice industry (Bizzo et al., 2009). EO of roses is rich in terpenic and sesquiterpenic compounds, which are widely explored by the pharmaceutical and cosmetic industries, mainly for their antioxidant capacity (Patrascu and Radoiu, 2016). Other compounds can also be found in other species of roses. In Rosa roxburghii, the EO presents significant amounts of linoleic acid, a powerful poly-unsaturated fatty acid possessing manifold health effects (Yang et al., 2020). The same oil also contains 2′-methylenebis (6-tert-butyl-4-methylphenol) considered a popular antioxidant and anti-aging agent. Phenols and flavonoids are compounds found in Rosa damascena, which presents antioxidant and free radical activity (Nikolova et al., 2019).
Worldwide, rose oil production is based on several species, such as Rosa damascena, Rosa gallica, Rosa centifolia, and Rosa alba L., the first two species being the most used in oil extraction (Kovatcheva et al., 2011). Among the several hybrid cultivars, the rose cv. Black Prince (also known as rose “Barcarolle”) (Avdic et al., 2016) is very widespread throughout Brazil due to its rusticity and beauty. It has medium productivity, high architecture and elongated buttons (Barbosa, 2008). However, there are no reports of oil production of this cultivar, which makes this fact one of the main attractions of the present study.
In Brazil, the production of roses occupies the first position among the cut flowers, corresponding to 30% of the national market (Junqueira and Peetz, 2017). However, one of the problems faced for the acquisition of EO is the low yield per plant, being necessary the use of extraction techniques that increase the yield per flower, or the use of different managements during the cultivation that stimulate the plant production (Pirbalouti et al., 2013).
A new strategy that is being investigated is the use of efficient microorganisms aimed at increasing these molecules of interest. These microorganisms, also known as plant growth promoters (PGP), are used for the most diverse purposes and are capable of providing several benefits (Vejan et al., 2016). In agriculture, the use of PGP has influenced the growth and development of plants, stimulating increased production, aiding in the control of pests and diseases, and activating enzymes capable of producing compounds that improve the quality of products obtained by plants inoculated with them (Saharan and Nehra, 2011; Numan et al., 2018). PGPs can also influence the production of secondary compounds by plants, since many of them have the ability to produce hormones such as auxins, cytokinins and gibberellins or even improve the absorption of nutrients such as nitrogen and phosphorus, which directly affects production and quality of EO from plants (Glick, 2012; Younis et al., 2015).
Bacteria are the most commonly used efficient microorganisms. Studies indicate that bacteria are able to assist plants in their development as well as stimulate the production of compounds that assist in plant defense, such as the production of secondary metabolites responsible for the plant defense system to external factors, which can influence the chemical composition of its aromatic compounds, such as EO (Xie et al., 2019).
In view of the discussed, the objective of this study was to select growth-promoting bacteria for rose cultivation and evaluate their influence on the production of EO.
Materials and Methods
Acquisition of Bacteria and Isolation of the Pathogen
Seven species of bacteria (Bacillus acidiceler, Bacillus amyloliquefaciens, Bacillus macauenses, Bacillus subtilis, Bacillus pumilus, Pantoea ananatis, Staphylococcus equorum) were isolated from strawberry plants and also from the Crop Collection of Agricultural Microbiology (CCMA) of the Federal University of Lavras, as shown in Table 1.
Table 1. Identification and origin of bacteria.
Protease Production
Protease production was determined by the method of Sgroy et al. (2009). Petri dishes with Skim Milk Agar (SMA) culture medium were inoculated with 10 μL of bacterial isolates previously cultivated in Luria-Bertami medium (LB) and incubated at 28°C. Halo formation around the colonies was observed after 24 h after inoculation.
Auxin Production
Cultures of bacteria activated in Petri dishes with medium 523 (10 g sucrose, 8 g casein hydrolysate, 4 g yeast extract, 2 g K2HPO4, 0.3 g MgSO4.7H2O, 15 g agar, 1000 mL distillated water) for 24 h were used. Bacteria colonies were transferred to a micro tube containing 1 mL of nutrient broth added of 0.1 g L−1 of L-tryptophan; the material was maintained in agitation for 3 days in the dark. After this period, the micro tubes were centrifuged at 1500 rpm for 3 min and a 0.5 mL aliquot was removed, which was added of 0.5 mL of Salkowiski's reagent. The reaction was incubated for 15 min in the dark, and then a pink or reddish color was observed which signifies the presence of auxins (Kado and Heskett, 1990).
Phosphate Solubilization Test
For this test, the bacteria were activated 24 h in advance. 10 μL of bacterial isolates grown in 523 liquid medium were inoculated in dishes containing the NBRIP medium (National Botanical Research Institute's Phosphate growth medium—India) (Nautiyal, 1999) which consists of glucose (10 g), Ca3(PO4)2 (5 g), MgCl2.6H2O (5 g), MgSO4.7H2O (0.25 g), KCl (0.2 g), (NH4)2SO4 (0.1 g), and agar (15 g). The material was incubated at 28°C with 12 h photoperiod. Halo formation was evaluated around the colonies after 24 h after inoculation.
Biological Nitrogen Fixation Test
This test was performed using 10 mL flasks containing 5 mL of semi-solid NFB medium, consisting of malic acid (5 g), K2HPO4 (0.5 g), MgSO4.7H2O (0.2 g), NaCl (0.1 g), CaCl2.2H2O (0.02 g), vitamin solution (1 ml), micronutrient solution (2 ml), FeEDTA (4 ml), bromothymol blue (2 ml), KOH (4.5 g), agar (2 g) and pH 6.8 (Döbereiner et al., 1995). Bacteria with 24 h of growth, grown in medium 523 were inoculated in the medium. An inoculation loop was used to collect bacteria and puncture the medium. Then, they were incubated at 28°C, making it possible to observe the halo formation in the upper side of the culture medium, indicating the nitrogen fixation by bacteria.
Effect of Bacteria on the Production of Roses
According to the growth-promoting tests, 4 (four) species of bacteria were selected and inoculated in roses of cultivar Black Prince to evaluate the bacterial effect on the productive characteristics of plants. To prepare the suspension, the bacteria were cultured for 48 h in nutrient broth and the concentration of each suspension was adjusted to 106 cells mL−1. For inoculation, the plants were pruned at 30 cm from the grafting site, removed from the pots and their roots washed until complete exposure. The inoculation in the plants was done by immersing the bare roots in the suspension for 1 h. After that time, the plants were planted in pots with a capacity of 10 L, containing commercial substrate Topstrato®.
The experiment was carried out in a greenhouse, in a randomized block design with 5 treatments (each selected bacteria plus a control that corresponded to plants inoculated with distilled water only) and 5 replicates. The experimental unit consisted of 4 plants per block.
The evaluations started at the first cutting of stems with flowers at the point of harvest (Figure 1). At that point, the floral buds is closed and it changes from a pointed shape to a cup shape. From the first to 5th day, the following phytotechnical characteristics were evaluated: diameter of the closed bud, diameter of the flower at the point of harvest, stem size, stem diameter, stem weight and total weight of petals per flower.
Figure 1. Flower bud opening stages. (A) Bud at the harvest point. (B) Completely open flower.
Plant Material and EO Extraction
Rose EO was extracted from flowers at the point of harvest. The plants were acquired from a local producer (Roselanche Floriculture—Garden and Floriculture Brandão, Barbacena, Minas Gerais State, Brazil). The fresh petals were taken to an oven with air circulation for drying at 30°C. After drying, they were weighed and the EO was obtained using ~100 g of dry rose petals with distilled water in a round bottom flask. The plant material was subjected to the hydrodistillation process using the modified Clevenger apparatus for a period of 4 h (Brasil, 2010). Three extractions were performed. EO was separated from the hydrolate by a pentane partition process. The organic part was dried using anhydrous sodium sulfate, filtered and rotary evaporated to eliminate the solvent. EO without solvent residues was protected from light and refrigerated.
Chemical Characterization of EO
The chemical constituents of EO were characterized by gas chromatography coupled to a mass spectrometer (GC-MS, Shimadzu Corporation, model QP2010 Plus, Kyoto, Japan) according to Adams (2007). The following conditions were performed: fused silica capillary column with phase connected to DB5 (30 mx 0.25 mm id, film thickness 0.25 μm); He 5.0 (White Martins, Rio de Janeiro, Brazil) as carrier gas and flow rate of 1 mL min−1. The injector temperature was 220°C and the detector temperature was 240°C. The injection volume of the sample was 0.5 μL diluted in hexane (1%) (Sigma-Aldrich®, St. Louis, MO, USA) with a division ratio of 1:100. Programmed temperature of 60°C increasing to 240°C at a rate of 3°C per min and from 240°C to 300°C at 10°C per min. The final temperature was maintained for 7 min. The operational parameters of the GC-MS followed the conditions: ionization potential (70 eV), ion source temperature (200°C), scan speed of 1000 Da sec−1, scan interval of 0.50 fragments sec−1. Mass analyzes were performed in full scan mode, ranging from 45 to 500 Da. The data regarding chemical constituents were acquired using LabSolutions LC/GC Workstation 2.72. The Van Den Dool and Kratz (1963) equation was used to calculate the retention index (IR = 100n + 100 [(tR (i)–tR (n))/(tR (n + 1) n), where tR (i), tR (n) and tR (n + 1) correspond to the retention times of the tested compounds and standards, respectively. The standards used were the homologous series of n-alkanes (nC8-nC18). The identification of compounds was based on comparison with the retention indices with those of the literature (Adams, 2007) and the mass spectra of the EO constituents with up to 95% similarity were compared with those of the mass spectra libraries FFNSC 1.2, NIST 107, and NIST 21. The quantification of volatile compounds was determined by gas chromatography with a flame ionization detector (DIC) (Shimadzu GC - 2010, Kyoto, Japan). The experimental parameters were the same used by GC-MS, with the exception of the detector temperature, which was 300°C. The percentages of compounds were calculated using the area normalization method.
Yield and Content of Extracted Oils
Due the low amount of extracted oil, the amount was estimated using the difference in weight of the bottle before and after the extraction of the EO. For this purpose, the amber bottles were weighed before extraction and at the end of the extraction process, after the complete evaporation of hexane at room temperature. The weight difference determined the amount of oil extracted. With these values, the yield for each treatment was determined and the content of EO was calculated according to the following formula proposed by Santos et al. (2004): Oil content (%) = [Weight of oil (g) x Dry petal biomass (g)]/100. The yield of EO was expressed in weight of oil per unit weight of plant material in Moisture Free Base (% w/w MFB).
Statistical Analysis of Data
The data were tested for normality and subsequently submitted to ANOVA. In case of significance, the means were compared by the Scott-Knott test (P ≤ 0.05), using the statistical software SISVAR version 5.2 (Ferreira, 2014).
Results and Discussion
The results of the growth promotion tests showed that the strains of Bacillus acidiceler, B. subtilis, B. pumilus and Staphylococcus equorum were efficient in the phosphate solubilization, protease production and nitrogen fixation tests (Table 2). The B. amyloliquefacies strain was efficient for phosphate solubilization and protease production and the B. macauenses and Pantoea ananatis strains were efficient only for phosphate solubilization and protease production, respectively. None of the strains of bacteria used showed the ability to produce auxins by the test evaluated.
Table 2. Production test of biochemical compounds by bacteria, evaluation of phosphate solubilization, protease production, biological nitrogen fixation, and auxin production.
Phosphate solubilization is a mechanism common to many microorganisms that are associated with plant roots. This solubilization capacity may be related to the decrease in the pH of the medium due to the production of organic acids (such as gluconic acid, oxalic acid, malonic acid, acetic acid, formic acid, citric acid, and succinic acid), which can dissolve the phosphate mineral, as a result of anion exchange, or chelate ions associated with phosphate (Chagas Junior et al., 2010; Tenga et al., 2019). Acidification of the medium is correlated with phosphorus solubilization by acid phosphatase activity, indicating that some microorganisms have developed enzymes capable of releasing inorganic phosphate from organophosphate complexes, in addition to the activity of this enzyme proving to be a good indicator for the mineralization of organic phosphorus (Kumar, 2016; Tenga et al., 2019). Likewise, the capacity for nitrogen fixation is common to many groups of microorganisms and their association due to its beneficial potential has been studied for application in the most varied agricultural crops (Mondani et al., 2019; Schmidt et al., 2019).
Regarding the production of roses, the application of bacteria did not influence the size of the bud before the flower opened (Table 3). However, plants treated with B. acidiceler, B. subtilis, and B. pumilus had higher diameter of open flower. An increase in the weight of the petals of plants treated with B. pumilus was also observed, showing a positive effect of this bacterium in relation to the others evaluated.
Table 3. Effect of growth-promoting bacteria on the bud diameter, flower diameter and weight of rose petals of the cultivar Black Prince.
Some growth-promoting bacteria can interfere with the production of hormones by plants, which can affect the structure of the roots, increasing their surface area, number and type (Ali et al., 2010; Saharan and Nehra, 2011). In addition to these factors, inoculation with endophytic bacteria can promote increased photosynthesis inducing greater production of carbohydrates by plants (Olanrewaju et al., 2017). In roses, the accumulation of carbohydrates in the flower stems can favor the durability of the flowers in the post-harvest. Thus, the application of endophytic microorganisms that aid in the accumulation of carbohydrates in the flower stems can improve the final quality of the roses, leading to greater durability of the cut stems (Moubayidin et al., 2009; Wang et al., 2014, 2019; Castanheira et al., 2017).
Plants treated with B. acidiceler and B. pumilus had a larger stem size (Table 4). The diameter of the stems was smaller in plants treated with S. equorum and B. pumilus, but only plants treated with S. equorum had lower weight of flower stems.
Table 4. Effect of growth-promoting bacteria on size, diameter and weight of stem of roses cv. Black Prince.
The yield of oil extracted from roses treated with B. pumilus was superior to other bacteria. However, a negative effect was observed on the yield of plants treated with B. acidiceler, in which the oil content was also lower in plants treated with this bacterium, with no difference in oil content between the plants treated with the other bacteria and the control (Table 5). However, it was not possible to obtain enough EO to perform the analysis on roses treated with the S. equorum bacterium through the methodology used for extraction.
Table 5. Effect of growth-promoting bacteria on yield and content of essential oil of roses cv. Black Prince.
The drying of the material can lead to decreases in the content of EOs and changes in its composition. Although freeze-drying is indicated due to less loss for EO amount, some species may have higher content of EOs when subjected to a convective drying process (Pirbalouti et al., 2013; Roslon et al., 2016).
In addition to phosphate solubilization and nitrogen fixation, growth-promoting microorganisms may be able to produce phytohormones, siderophores and antibiotics, and they are also capable of inducing systemic resistance in the plant (Sgroy et al., 2009). In addition, plants can also interact with these organisms by increasing their growth, since the increase in the amount of EOs is often related to the growth of plant organs due to greater absorption of nutrients; the quality of the oils can also be influenced by the activation of metabolic routes due to the interaction of the plant with microorganisms (Maji et al., 2013; Singh et al., 2019).
B. subtilis strains have the potential to stimulate plant biomass production even under adverse field conditions, such as in saline soils, and to increase chlorophyll production and soluble protein content (Wang et al., 2018). In addition, the application of B. subtilis strains positively influenced the growth and production of crops such as cotton, brachypodium grass (Brachypodium distachyon), arabidopsis (Arabidopsis thaliana) and wheat (Triticum aestivum) (Ali, 2015; Gagné-Bourque et al., 2015; Wang et al., 2018; Andrees et al., 2019).
For the chemical composition of EO of roses, there was a significant difference according to each bacterium used in the treatment of plants (Table 6, Figure 2). The smallest number of components was found in the oils of plants treated with B. acidiceler. However, plants treated with B. pumilus and B. subtilis had higher number of compounds, in which the chemical constituents of carophyllene oxide, α-selinene, limonene, α-farnecene appeared only in plants treated with B. pumilus and teaspiran only in flowers treated with B. subtilis.
Table 6. Effect of growth-promoting bacteria on chemical composition of essential oil of roses cv. Black Prince extracted by hydro-distillation.
Figure 2. Chromatography of essential oils of rose cv. Black Prince relating the retention time and intensity of each component, according to each growth-promoting bacteria, being: (1) Myrtanol, (2) Cetyl alcohol, (3) Eicosane, (4) Hexadecanoic acid, (5) Hexenol, (6) Pentadecanol, (7) Docosane, (8) Tetracosane, (9) Pentacosane, (10) Caryophyllene, (11) α-Selinene, (12) Limonene, (13) α-Farsene, (14) Heneicosane, (15) Heptadecyl alcohol, (16) Theaspirane.
Limonene is related to the strong smell of orange, which constitutes the final citrus aspect of the aroma of the species under study. This compound has an insecticidal action and can confer resistance to insects in some plants, being widely used in flavor and fragrance industries (Thomas and Bessière, 1989; Sowndhararajan et al., 2015). A-selinene, on the other hand, is a sesquiterpene that is not part of the smell composition of the oils where it is present, but it is part of the chemical composition of the EO of several plants such as guamirim (Calyptranthes concinna), citronella (Cymbopongon nardus), and tiririca (Cyperus rotundus) (Limberger et al., 2002; Lawal and Oyedeji, 2009; Silva et al., 2011).
A-farnesene is a sesquiterpene related to the defense of plants to aphids, in addition to conferring the flavor of some vegetables, being found naturally coating fruits such as apples and pears. In EOs, this compound is also related to the smell of floral-green or woody, being one of the major constituents of ginger oil (Harbone, 1997; Andrade et al., 2012).
Similar to α-farnesene, carophyllene oxide is also a major constituent of EOs from some plants that may contain toxic compounds. This compound has the potential to inhibit the growth of Gram-positive bacteria, and is also toxic to ants and fungi associated with them. In addition, the carophylene oxide found in EOs has already been associated with analgesic and anti-inflammatory action and can induce apoptosis in lymphoma and neuroblastoma cells (Norouzi-Arasi et al., 2006; Judzentienea et al., 2010; Sain et al., 2014). The presence of this compound may indicate an improvement in the medicinal properties of rose oil, as well as influencing their defense against leaf-cutting ants.
The alcohols and esters are the main constituents found in the EO of several varieties and species of roses, configuring more than 80% of the key components of its aromas in some of those plants, where the alcohols are the components used in the evaluation of the quality of the EO of roses (Xiao et al., 2018).
The compounds myrtanol, cetyl alcohol, eicosane, hexadecanoic acid, hexenol, docosane, tetracosan, pentacosane, heneicosane, and heptadecyl alcohol are part of the volatile compounds of EO of rose species intended for the extraction of EOs. However, its presence and quantity in the oil can vary depending on the management, environmental conditions, methodology and time of extraction (Özel et al., 2004; Joichi et al., 2005; Xiao et al., 2018).
The promotion of plant growth has also been proven in strains of B. pumilus, showing its efficiency in the growth, development and production of plants, as well as in resistance to some environmental stresses, such as salinization, drought, and tolerance to heavy metals. Those strains also reduce reactive oxygen species, increasing the content of secondary metabolites and increasing the activity of antioxidant enzymes (Khan et al., 2016; Sirajuddin et al., 2016; Xie et al., 2019). The potential of these microorganisms to stimulate the production and accumulation of secondary metabolites may explain the increase in the EO content in roses and the different compounds present in the chemical composition of the oil extracted from roses inoculated with B. pumilus.
Conclusions
B. acidiceler, B. pumilus and B. subtilis improved the phytotechnical characteristics of roses. Among bacteria, B. pumilus increased the essential oil content as well as positively changed the chemical composition of the extracted essential oil from roses cv. Black Prince.
Data Availability Statement
The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.
Author Contributions
NA and JD: research design, experiments administration, supervision, and writing. RB and MMAP: experiments conduction (bacterial analysis) and data discussion. BB, MC, and VB: experiments conduction (rose analysis) and data discussion. MP: visualization and data discussion. RR: writing and critical revision. All authors read and approved the final manuscript.
Funding
This work was supported by the National Council for Scientific and Technological Development (CNPq - Brazil).
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
The authors would like to thank the National Council for Scientific and Technological Development (CNPQ/Brazil) for scholarships and financial support and also to the Electronic Microscopy Laboratory, the Organic Chemistry Laboratory/Essential Oils, and the Chemical Analysis and Prospecting Center of the Federal University of Lavras for providing the equipment for the analyzes.
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Keywords: Bacillus sp, biological activity, content, efficient microorganisms, Rosa hybrida L.
Citation: Araújo NAF, Brandão RM, Barguil BM, Cardoso MdG, Pasqual M, Rezende RALS, Pereira MMA, Buttrós VHT and Dória J (2020) Plant Growth-Promoting Bacteria Improve Growth and Modify Essential Oil in Rose (Rosa hybrida L.) cv. Black Prince. Front. Sustain. Food Syst. 4:606827. doi: 10.3389/fsufs.2020.606827
Received: 15 September 2020; Accepted: 04 November 2020;
Published: 18 December 2020.
Edited by:
Everlon Cid Rigobelo, São Paulo State University, BrazilReviewed by:
Akbar Karami, Shiraz University, IranYu Han, Beijing Forestry University, China
Marta Monder, Polish Academy of Sciences Botanical Garden, Poland
Copyright © 2020 Araújo, Brandão, Barguil, Cardoso, Pasqual, Rezende, Pereira, Buttrós and Dória. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Joyce Dória, am95Y2UuZG9yaWEmI3gwMDA0MDt1ZmxhLmJy