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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Biotechnology
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1503346

The SEPALLATA Like Gene HrSEP1 in Hippophae rhamnoides Regulates Flower Development By Interacting With Other MADS Box Subfamily Genes

Provisionally accepted
Di Cong Di Cong 1*Xue Zhao Xue Zhao 2*Chang Ni Chang Ni 1*Mengru Li Mengru Li 1*Luwen Han Luwen Han 1*Jianlin Cheng Jianlin Cheng 1*Hongzhang Liu Hongzhang Liu 1*Huijing Liu Huijing Liu 1*Dan Yao Dan Yao 1*Guoshuang Chen Guoshuang Chen 3*Shuying Liu Shuying Liu 1*
  • 1 Jilin Agriculture University, Changchun, China
  • 2 Leshan Food and Drug Inspection and Testing Center, Leshan, China
  • 3 Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences (CAS), Changchun, Hebei Province, China

The final, formatted version of the article will be published soon.

    MADS-box genes are classified into five categories: ABCDE, including SEP1, SEP2, SEP3, SEP4, and other homologous genes, which play important roles in floral organ development. In this study, the cDNA sequence of the HrSEP1 gene was cloned by RT-PCR and confirmed that this gene belongs to the MADS-box gene family. In addition, subcellular localization experiments showed that the HrSEP1 protein was localized in the nucleus. We verified the interaction of HrSEP1 with HrSOC1, HrSVP, and HrAP1 using yeast two-hybrid and bimolecular fluorescence complementation assays. These genes jointly regulate the growth and development of floral organs. We also found a strong synergy between HrSEP1 and AP1 genes in sepals, petals, and stamens by transgenic methods and fluorescence quantitative PCR, suggesting that HrSEP1 and AP1 may co-regulate the development of these structures. In conclusion, the expression of HrSEP1 has a certain effect on the development of floral organs, and these findings lay the foundation for further research on the biological functions of MADS transcription factors in Hippophae rhamnoides.

    Keywords: Hippophae rhamnoides, MADS-box genes, yeast two-hybrid, bimolecular fluorescence complementation, genetic transformation

    Received: 28 Sep 2024; Accepted: 30 Dec 2024.

    Copyright: © 2024 Cong, Zhao, Ni, Li, Han, Cheng, Liu, Liu, Yao, Chen and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Di Cong, Jilin Agriculture University, Changchun, China
    Xue Zhao, Leshan Food and Drug Inspection and Testing Center, Leshan, China
    Chang Ni, Jilin Agriculture University, Changchun, China
    Mengru Li, Jilin Agriculture University, Changchun, China
    Luwen Han, Jilin Agriculture University, Changchun, China
    Jianlin Cheng, Jilin Agriculture University, Changchun, China
    Hongzhang Liu, Jilin Agriculture University, Changchun, China
    Huijing Liu, Jilin Agriculture University, Changchun, China
    Dan Yao, Jilin Agriculture University, Changchun, China
    Guoshuang Chen, Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences (CAS), Changchun, 130102, Hebei Province, China
    Shuying Liu, Jilin Agriculture University, Changchun, China

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