Intraocular human cytomegaloviruses of ocular diseases are distinct from those of viremia and are capable of escaping from innate and adaptive immunity by exploiting HLA-E-mediated peripheral and central tolerance
- 1Department of Ophthalmology, Kyushu University, Fukuoka, Japan
- 2Department of Ocular Pathology and Imaging Science, Kyushu University, Fukuoka, Japan
- 3Ocular inflammation and Immunology, Singapore Eye Research Institute, Singapore, Singapore
- 4Ophthalmology and Visual Sciences Academic Clinical Program, Duke-NUS Medical School, Singapore, Singapore
- 5Department of Infection Metagenomics, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
- 6Integrated Frontier Research for Medical Science Division, Institute for Open and Transdisciplinary Research Initiatives (OTRI), Osaka University, Osaka, Japan
- 7Department of Microbiology and Immunology, Graduate School of Medicine, Yamaguchi University, Yamaguchi, Japan
- 8Department of Molecular Immunology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
- 9Genome Medical Science Project, National Center for Global Health and Medicine, Tokyo, Japan
- 10Department of Ophthalmology, The University of Tokyo Hospital, Tokyo, Japan
- 11Department of Ophthalmology, Jichi Medical University Saitama Medical Center, Saitama, Japan
- 12Department of Ophthalmology, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan
- 13Department of Ophthalmology, Japan Community Health Care Organization Hospital, Osaka, Japan
- 14Department of Medicine and Biosystemic Science, Kyushu University Graduate School of Medical Science, Fukuoka, Japan
- 15Department of Ophthalmology, Gunma University Graduate School of Medicine, Gunma, Japan
- 16Department of Medicine, Washington University School of Medicine, St. Louis, MO, United States
- 17Center for Research and Education on Drug Discovery, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
- 18Laboratory of Biomolecular Science, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
- 19Global Station for Biosurfaces and Drug Discovery, Hokkaido University, Sapporo, Japan
- 20Bursky Center for Human Immunology and Immunotherapy Programs, Washington University, St. Louis, MO, United States
- 21Singapore Institute for Clinical Sciences (SICS), Agency for Science, Technology and Research, ASTAR, Singapore, Singapore
- 22Department of Pediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
- 23Department of Pediatrics, National University Health System, Singapore, Singapore
- 24Immunology Programme, Life Sciences Institute, National University of Singapore, Singapore, Singapore
- 25National University Singapore Medicine Immunology Translational Research Programme, National University of Singapore, Singapore, Singapore
- 26International Research Center for Medical Sciences, Kumamoto University, Kumamoto, Japan
by Shirane M, Yawata N, Motooka D, Shibata K, Khor S-S, Omae Y, Kaburaki T, Yanai R, Mashimo H, Yamana S, Ito T, Hayashida A, Mori Y, Numata A, Murakami Y, Fujiwara K, Ohguro N, Hosogai M, Akiyama M, Hasegawa E, Paley M, Takeda A, Maenaka K, Akashi K, Yokoyama WM, Tokunaga K, Yawata M and Sonoda K-H (2022) 13:1008220. doi: 10.3389/fimmu.2022.1008220
In the published article, there were errors. In the original article, there was an error in the description of the Reverse primer sequence in Materials and methods.
A correction has been made to Materials and methods, “Deep amplicon sequencing of CMV-UL40 genomic DNA”.
This sentence previously stated:
“The following forward and reverse primers were used: Forward, 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCAACAGTCGGCAGAATGAAC-3′ and Reverse, 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCTGGAACACGACGCATA-3’.”
The corrected sentence appears below:
“The following forward and reverse primers were used: Forward, 5′-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCAACAGTCGGCAGAATGAAC-3′ and Reverse, 5′-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGCTGGAACACGAGCGGACATA-3’.”
In the original article, there was another error in the description of the concentration of the anti-HLA-E antibody in Materials and methods.
A correction has been made to Materials and methods, “Immunohistochemistry analysis”.
This sentence previously stated:
“After antigen retrieval with boiling citrate buffer (pH 6.0), the sections were incubated with 5% skim milk for 1 h at room temperature to prevent nonspecific binding and stained with 10 μL/mL anti-HLA-E antibody [MEM-E/02] (Abcam; Cambridge, UK) or IgG from mouse serum (Sigma-Aldrich) overnight at 4°C.”
The corrected sentence appears below:
“After antigen retrieval with boiling citrate buffer (pH 6.0), the sections were incubated with 5% skim milk for 1 h at room temperature to prevent nonspecific binding and stained with 10 μg/mL anti-HLA-E antibody [MEM-E/02] (Abcam; Cambridge, UK) or IgG from mouse serum (Sigma-Aldrich) overnight at 4°C.”
The authors apologize for these errors and state that these do not change the scientific conclusions of the article in any way. The original article has been updated.
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Keywords: human cytomegalovirus, HLA-E, UL40, natural killer cells, HLA class I, NKG2A, CMV viremia, CMV retinitis
Citation: Shirane M, Yawata N, Motooka D, Shibata K, Khor S-S, Omae Y, Kaburaki T, Yanai R, Mashimo H, Yamana S, Ito T, Hayashida A, Mori Y, Numata A, Murakami Y, Fujiwara K, Ohguro N, Hosogai M, Akiyama M, Hasegawa E, Paley M, Takeda A, Maenaka K, Akashi K, Yokoyama WM, Tokunaga K, Yawata M and Sonoda K-H (2023) Corrigendum: Intraocular human cytomegaloviruses of ocular diseases are distinct from those of viremia and are capable of escaping from innate and adaptive immunity by exploiting HLA-E-mediated peripheral and central tolerance. Front. Immunol. 13:1124440. doi: 10.3389/fimmu.2022.1124440
Received: 15 December 2022; Accepted: 16 December 2022;
Published: 05 January 2023.
Edited and Reviewed by:
Michael G. Brown, University of Virginia, United StatesCopyright © 2023 Shirane, Yawata, Motooka, Shibata, Khor, Omae, Kaburaki, Yanai, Mashimo, Yamana, Ito, Hayashida, Mori, Numata, Murakami, Fujiwara, Ohguro, Hosogai, Akiyama, Hasegawa, Paley, Takeda, Maenaka, Akashi, Yokoyama, Tokunaga, Yawata and Sonoda. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Nobuyo Yawata, eWF3YXRhLm5vYnV5by43MThAbS5reXVzaHUtdS5hYy5qcA==