About this Research Topic

Background

Human pluripotent stem cells (PSCs), including embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), can self-renew indefinitely, and differentiate into any cell type in the body. Intensive research in the past two decades that exploited the properties of PSCs has opened up new frontiers of basic and translational biomedical research. Human PSCs have enabled the modeling of disease pathogenesis in the human context, and have helped to illuminate mechanisms of monogenic disease, complex disease, cancer, and, most recently, emerging infectious disease such as COVID-19. Lately, the striking ability of PSCs to self-organize has engendered three-dimension (3D) models of human development (e.g., blastoids, gastruloids, assembloids, and organoids), which have hitherto remained a black box to mechanistic studies. Genome editing technology, whose development has intertwined with that of PSCs, has become an integral and synergistic component of PSC-based models by providing, for examples, isogenic controls, lineage tracing reporters, and genome-wide genetic screens. Human PSC models aided by genome editing technologies and single-cell OMICs analysis (scRNA-seq, scATAC-seq, CUT&TAG, spatial transcriptomics, etc.) promise to deliver unprecedented insights into human development and disease pathogenesis, which serve as the basis for future precision therapies.

Many challenges remain to be addressed before the full potentials of human PSC models can be realized. This Research Topic is aimed at identifying and tackling some of the roadblocks to the use of stem cell-based models in basic and translational research. For example, the 2D and 3D stem cell models generally suffer from low differentiation efficiency and high experimental variability. There is an urgent need for best practice guidelines and evaluation standards to ensure reproducibility of results obtained from different models. Advances in bioengineering methods for monitoring and controlling cellular environments (pCO2, pO2, pH, substrate stiffness, and hydrodynamic forces) are expected to improve cellular differentiation and reproducibility. Such new methods will go hand in hand with mechanistic studies aimed at understanding the sources of experimental variability (e.g., inter-variability among iPSC lines derived from different donors and intra-variability among iPSC clones derived from the same donor) and together they can help to standardize differentiation protocols for future clinical translation. The recent revelation of various untended genotoxic effects of genome editing tools has implications in the interpretation of experimental findings and the safety of genome edited stem cells in the clinic. New methods and mechanistic studies aimed at characterizing and understanding the side effects of genome editing (e.g., on-target large deletions and complex rearrangements, chromosomal loss, and off-target effects), respectively, will be of broad interest to the field. Exciting research advances at the above-mentioned fronts will undoubtedly improve the translational values of stem cell models.

We welcome contributions, from Original Research to Review Articles, that cover advances (not limited to) the following areas:
• Effects of culture environmental factors (gases, pH, and mechanical factors) on the differentiation, maturation, and survival of 2D and 3D stem cell models;
• Method to monitor and evaluate 2D and 3D stem cell models;
• Methods to control culture environments or to improve cellular performance by engineering stem cell models;
• Heterogeneity, variability, and reproducibility of 2D and 3D stem cell models;
• Methods to characterize unintended genotoxic effects of genome editing tools;
• Development of new stem cell-based models of early human development and diseases;
• High-throughput sequencing and computational approaches to study human development and disease.



Dr. Ying Gu is employed by BGI-Research, all other Topic Editors declare no conflicts of interest.

Keywords: stem cells models, 2D stem cell models, 3D stem cell models, pluripotent stem cells

Important note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.

Frequently asked questions

• What is a Research Topic?

  Frontiers' Research Topics are collaborative hubs built around an emerging theme.Defined, managed, and led by renowned researchers, they bring communities together around a shared area of interest to stimulate collaboration and innovation.

  Unlike section journals, which serve established specialty communities, Research Topics are pioneer hubs, responding to the evolving scientific landscape and catering to new communities.

• Why does Frontiers publish Research Topics? 

  The goal of Frontiers' publishing program is to empower research communities to actively steer the course of scientific publishing. Our program was implemented as a three-part unit with fixed field journals, flexible specialty sections, and dynamically emerging Research Topics, connecting communities of different sizes and maturity.

  Research Topics originate from the scientific community. Many of our Research Topics are suggested by existing editorial board members who have identified critical challenges or areas of interest in their field.

• Why are Research Topics important for journals?

  As an editor, Research Topics will help you build your journal, as well as your community, around emerging, cutting-edge research. As research trailblazers, Research Topics attract high-quality submissions from leading experts all over the world.

  A thriving Research Topic can potentially evolve into a new specialty section if there is sustained interest and a growing community around it.

• How do we ensure the quality of articles within Research Topics?

  Each Research Topic must be approved by the specialty chief editor, and it falls under the editorial oversight of our editorial boards, supported by our in-house research integrity team. The same standards and rigorous peer review processes apply to articles published as part of a Research Topic as for any other article we publish.

  In 2023, 80% of the Research Topics we published were edited or co-edited by our editorial board members, who are already familiar with their journal's scope, ethos, and publishing model. All other topics are guest edited by leaders in their field, each vetted and formally approved by the specialty chief editor.

• What are the benefits of publishing my article in a Research Topic?

  Publishing your article within a Research Topic with other related articles increases its discoverability and visibility, which can lead to more views, downloads, and citations. Research Topics grow dynamically as more published articles are added, causing frequent revisiting, and further visibility.

  As Research Topics are multidisciplinary, they are cross-listed in several fields and section journals – increasing your reach even more and giving you the chance to expand your network and collaborate with researchers in different fields, all focusing on expanding knowledge around the same important topic.

  Our larger Research Topics are also converted into ebooks and receive social media promotion from our digital marketing team.

   
• What types of articles can be published in a Research Topic?

  Frontiers offers multiple article types, but it will depend on the field and section journals in which the Research Topic will be featured. The available article types for a Research Topic will appear in the drop-down menu during the submission process.

  Check available article types here 

• Can I suggest a new Research Topic? 

  Yes, we would love to hear your ideas for a topic. Most of our Research Topics are community-led and suggested by researchers in the field. Our in-house editorial team will contact you to talk about your idea and whether you’d like to edit the topic. If you’re an early-stage researcher, we will offer you the opportunity to coordinate your topic, with the support of a senior researcher as the topic editor. 

  Suggest your topic here 

• Who leads a Research Topic? 

  A team of guest editors (called topic editors) lead their Research Topic. This editorial team oversees the entire process, from the initial topic proposal to calls for participation, the peer review, and final publications.

  The team may also include topic coordinators, who help the topic editors send calls for participation, liaise with topic editors on abstracts, and support contributing authors. In some cases, they can also be assigned as reviewers.

• What is the role of a topic editor?

  As a topic editor (TE), you will take the lead on all editorial decisions for the Research Topic, starting with defining its scope. This allows you to curate research around a topic that interests you, bring together different perspectives from leading researchers across different fields and shape the future of your field. 

   

  You will choose your team of co-editors, curate a list of potential authors, send calls for participation and oversee the peer review process, accepting or recommending rejection for each manuscript submitted.

• What support will I receive when editing a new Research Topic? 

  As a topic editor, you're supported at every stage by our in-house team. You will be assigned a single point of contact to help you on both editorial and technical matters. Your topic is managed through our user-friendly online platform, and the peer review process is supported by our industry-first AI review assistant (AIRA).

• As a junior researcher, can I be involved in editing a Research Topic? 

  If you’re an early-stage researcher, we will offer you the opportunity to coordinate your topic, with the support of a senior researcher as the topic editor. This provides you with valuable editorial experience, improving your ability to critically evaluate research articles and enhancing your understanding of the quality standards and requirements for scientific publishing, as well as the opportunity to discover new research in your field, and expand your professional network.

• Can I get a certificate for editing a Research Topic?

  Yes, certificates can be issued on request. We are happy to provide a certificate for your contribution to editing a successful Research Topic.

• Who will I collaborate with?

  Research Topics thrive on collaboration and their multi-disciplinary approach around emerging, cutting-edge themes, attract leading researchers from all over the world.

• How long does it take to publish a Research Topic?

  As a topic editor, you can set the timeline for your Research Topic, and we will work with you at your pace. Typically, Research Topics are online and open for submissions within a few weeks and remain open for participation for 6 – 12 months. Individual articles within a Research Topic are published as soon as they are ready.

  Find out more about our Research Topics

• What fee support is available to authors publishing in a Research Topic?

  Our fee support program ensures that all articles that pass peer review, including those published in Research Topics, can benefit from open access – regardless of the author's field or funding situation.

  Authors and institutions with insufficient funding can apply for a discount on their publishing fees. A fee support application form is available on our website.

• Will there be a printed version of my Research Topic?

  In line with our mission to promote healthy lives on a healthy planet, we do not provide printed materials. All our articles and ebooks are available under a CC-BY license, so you can share and print copies.