About this Research Topic
Pluripotent stem cells (PSCs) are invaluable for studying organogenesis and developmental processes such as osteogenesis, adipogenesis, and cardiogenesis. These cells provide a controlled in vitro platform to mimic early developmental stages and differentiate into diverse cell lineages, mirroring natural organogenesis. Understanding the molecular mechanisms governing differentiation is crucial for addressing developmental disorders and diseases.
Traditional two-dimensional differentiation studies using human pluripotent stem cells (hPSCs) and animal models have elucidated developmental pathways—from pluripotent states through intermediate stages to mature, specialized cells or organs. These studies have enhanced our understanding of organ development by replicating endogenous signaling cues crucial for differentiation in vivo. However, monolayer differentiation methods often fail to fully replicate the complex cell-cell interactions observed in natural development.
This collection aims to spotlight recent advancements in three-dimensional stem cell differentiation, specifically organoid culture techniques. Organoids, miniature versions of organs grown in vitro, provide more physiologically relevant models compared to traditional monolayer cultures. These models offer insights into developmental processes and disease mechanisms that are closer to natural conditions, paving the way for improved therapeutic strategies and disease modeling.
Overall, leveraging three-dimensional organoid culture techniques allows for a deeper exploration of developmental biology, enhancing our ability to study and potentially treat human diseases associated with developmental abnormalities. Leveraging these advanced techniques allows for a deeper exploration of developmental biology, enhancing our ability to study and potentially treat human and animal diseases associated with developmental abnormalities. Therefore, this research topic seeks to present a comprehensive, up-to-date collection of studies focusing on stem cell differentiation and its applications in animal research. We invite contributions in the form of Original Research Articles, Reviews, Mini-Reviews, Systematic Reviews, Perspectives, Commentaries, Data Notes, and Technical Notes. Topics of interest include, but are not limited to:
• Comprehensive studies highlighting recent advancements, challenges, and future directions in stem cell differentiation methodologies.
• Detailed studies presenting new findings and techniques in the development and application of cell culture models for various tissues and organs.
• Studies demonstrating the use of cell cultures to model developmental diseases, providing insights into disease mechanisms and potential therapeutic approaches.
• Research comparing the efficacy, fidelity, and physiological relevance of 2D versus advanced stem cell differentiation approaches.
• Studies introducing novel techniques and technologies to investigate the complex cell-cell and cell-matrix interactions within culture systems.
Traditional two-dimensional differentiation studies using human pluripotent stem cells (hPSCs) and animal models have elucidated developmental pathways—from pluripotent states through intermediate stages to mature, specialized cells or organs. These studies have enhanced our understanding of organ development by replicating endogenous signaling cues crucial for differentiation in vivo. However, monolayer differentiation methods often fail to fully replicate the complex cell-cell interactions observed in natural development.
This collection aims to spotlight recent advancements in three-dimensional stem cell differentiation, specifically organoid culture techniques. Organoids, miniature versions of organs grown in vitro, provide more physiologically relevant models compared to traditional monolayer cultures. These models offer insights into developmental processes and disease mechanisms that are closer to natural conditions, paving the way for improved therapeutic strategies and disease modeling.
Overall, leveraging three-dimensional organoid culture techniques allows for a deeper exploration of developmental biology, enhancing our ability to study and potentially treat human diseases associated with developmental abnormalities. Leveraging these advanced techniques allows for a deeper exploration of developmental biology, enhancing our ability to study and potentially treat human and animal diseases associated with developmental abnormalities. Therefore, this research topic seeks to present a comprehensive, up-to-date collection of studies focusing on stem cell differentiation and its applications in animal research. We invite contributions in the form of Original Research Articles, Reviews, Mini-Reviews, Systematic Reviews, Perspectives, Commentaries, Data Notes, and Technical Notes. Topics of interest include, but are not limited to:
• Comprehensive studies highlighting recent advancements, challenges, and future directions in stem cell differentiation methodologies.
• Detailed studies presenting new findings and techniques in the development and application of cell culture models for various tissues and organs.
• Studies demonstrating the use of cell cultures to model developmental diseases, providing insights into disease mechanisms and potential therapeutic approaches.
• Research comparing the efficacy, fidelity, and physiological relevance of 2D versus advanced stem cell differentiation approaches.
• Studies introducing novel techniques and technologies to investigate the complex cell-cell and cell-matrix interactions within culture systems.
Keywords: Pluripotent stem cells, Organoids, Stem Cell Differentiation, regenerative Medicine
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