About this Research Topic
The goal is to present and disseminate articles with easily implemented information regarding the use of non-animal products in different experimental settings involving cell lines. These articles aim to encourage and stimulate to the true use of animal-free experimental approaches. The first and main goal is to help researchers to abandon FBS but also other animal-derived sera. Additionally, we aim to supply research data that can lead to the elimination of use of Matrigel isolated from mice harboring Engelbreth-Holm-Swarm mouse tumors as well as trypsin and other animal-derived materials. All submitted research should be also without the use of live animals.
In this Research Topic, we welcome submissions of original research, reviews, mini-reviews, methods, perspectives, community case studies, conceptual analysis, data reports, policy briefs, brief research reports, general commentaries, and opinions.
Areas to be covered in this Research Topic may include but are not limited to:
• Validation of OECD test guidelines using animal-product-free media, preferably using open-source media to emphasis on advancing reproducible methodologies.
• Explore strategies for the replacement of FBS in cosmetic testing strategies. Emphasizing the importance of ethics in the cosmetic industry to making cosmetics truly animal-free.
• Replacements of Matrigel and other animal-derived hydrogels.
• Replacements of animal-derived trypsin in cell culture practices and transition to using alternative animal-free dissociation reagent sources.
• Establishment of cell lines in animal-product free media preferably using (non-proprietary) open-source media. This would address the entire cell culture process including passaging and cell banking.
• Stem cell research free of animal-derived materials.
• Reevaluation of already existing articles where the only used animal-derived product is bovine serum albumin (BSA). We would strongly urge the authors to substitute BSA with human serum albumin (HSA).
Keywords: animal-free alternatives, new approach methodologies, toxicology, cell culture
Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.