Advancements in Mitochondrial Electrophysiology: Understanding Ion Channel Function and Structure

Mitochondrial electrophysiology is a field that has significantly evolved since the discovery of ionic currents in mitochondria nearly fifty years ago. Initially, the reconstitution of sub-mitochondrial particles into artificial lipid membranes and the patch-clamp technique of mitoplasts were pivotal in unveiling the properties and functions of mitochondrial ion channels. These channels, including various potassium channels, the calcium uniporter, and the mitochondrial permeability transition pore, play crucial roles in both physiological and pathological processes. Despite advancements, the molecular identity of some channels remains elusive, and the field continues to grapple with methodological challenges. Recent studies have expanded our understanding of these channels, particularly through the use of targeted site-specific mutations and drug interactions, yet there remains a need for further exploration into their regulation, function, and structure.

This Research Topic aims to delve deeper into the progression of mitochondrial electrophysiology, from the initial descriptions of ionic currents to the intricate studies of channel regulation and function. The primary objectives include understanding the impact of ion channel mutations on mitochondrial function and exploring the potential for drug interventions to address altered channel properties. By addressing these questions, the research seeks to enhance our comprehension of mitochondrial ion channels and their roles in health and disease.

To gather further insights in mitochondrial electrophysiology, we welcome articles addressing, but not limited to, the following themes:
- A retrospection of the first electrophysiological experiments on mitochondria;
- Structural and functional considerations when investigating ion channels utilizing mitochondrial electrophysiology;
- Understanding ion channel mutations and their impact on mitochondrial electrophysiology;
- Delving into the significance of varying mitochondrial ion channels in the context of physiological and pathological states;
- Acknowledging technical challenges faced during mitochondrial electrophysiology studies;
- A critical evaluation of electrophysiological techniques applied to inspect mitochondrial channels;
- An inclusive overview of the advantages and limitations of mitochondrial electrophysiology;
- Structural insights in the functioning and membrane arrangement of mitochondrial channels;
- Demonstrated or putative roles of mitochondrial channels in the bioenergetics pathways.

Prof. Vito De Pinto is stockholder of a start up spinoff of University of Catania (weMitobiotech).
The other Topic Editors declare no conflict of interest with regards to the Research Topic subject.

Keywords: mitochondria, ion channels, mitochondrial electrophysiology, methods and applications in mitochondrial electrophysiology, ion channel mutations

Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.