About this Research Topic
With the advances in spectroscopic methods, various chemical probes have been developed for studying a wide range of biological and biochemical processes. Spectroscopic probes are designed chemical substances that interact with the components of biological systems, analytes and environmental compounds, resulted in modifications in their spectroscopic content (fluorescent, absorption and chemiluminescent), and provide information about behaviour, structure, and function of the investigated systems.
Studies have shown that there are various challenges in designing effective spectroscopic probes for bioanalysis. Any spectroscopic probe should have acceptable signal-to-noise ratio to detect target signals in the presence of noise when they are applied to monitor biological systems. Indeed, challenges with sensitivity of designed spectroscopic probes for specific target is another challenge to detect small changes in biological systems. Beyond this, to collect accurate signals, biosensing and bioimaging, during the usage of spectroscopic probes, these probes should not be toxic because they may harm biological systems and increase the chance of false results. Specific and sensitive spectroscopic probes should have distinct and detectable spectral properties to identify the desired signals from noises, therefore accurate design of such chemical probes requires understanding the chemical nature of these probes and chemical substances that will be used to design spectroscopic probes. Therefore, the rational design of spectroscopic probes not only increases their effectiveness for bioanalysis but significantly also decreases the total cost and amount of chemicals required to develop and design these probes. Although designing spectroscopic probes for bioanalysis is a multi-step and sophisticated process due to the complexity of biological systems, understanding the essential aspects of target systems that should be targeted for spectroscopic probe design, along with the chemistry of probes can expedite the development of these probes and facilitate the understanding of the biochemistry, behaviour, and structure of biological systems.
Studies have shown that the challenges in spectroscopic probe design are mainly related to the application of these probes. In the case of spectroscopic probes for bioanalysis, sensitivity, high reaction rates, high resolution outputs, acceptable solubility, safety, simplified working mechanisms, and simplified optimization are critical factors that influence the quality of designed spectroscopic probes. Due to the complexity of biological systems, any spectroscopic probe that used for monitoring such systems should show high selectivity and sensitivity to produce high-quality results. However, achieving such effective and validate results requires applying rationale methods and appropriate chemical agents in designing spectroscopic probes for bio-analysis. The growing body of evidence suggests that coupling of advanced analytical methods and imaging processes can significantly increase the quality of spectroscopic probes outputs. In this regard, this Research Topic aims to cover all innovations and new trends in developing new methods to design spectroscopic probes with high selectivity and sensitivity, good solubility in different biosystems, and with fewer or no side effects for the target biosystems. We highly appreciate submissions that address current challenges in designing synthetic spectroscopic probes for bioanalysis as well as advanced computational studies on the mode of action of spectroscopic probes are also welcome to this Research Topic.
Define the scope of the Research Topic, listing specific themes you would like contributors to address. Indicate the types of manuscripts you are interested in.
We welcome Original Research, Review, Mini Review and Perspective articles on themes including:
• Tackling methodological barriers in developing spectroscopic probes
• Evaluation of stability, biocompatibility and the recognition ability of the spectroscopic probes
• New switches to produce spectroscopic signals
• Computational advancements in spectroscopic probe design, improving analytical performance of spectroscopic probes for bioanalysis
• Modifying chemical structure of current materials used as spectroscopic probes to improve their efficacy with applications in oncology, inflammation infection, cardio imaging, neuroinflammation and neuro-oncology.
Studies have shown that there are various challenges in designing effective spectroscopic probes for bioanalysis. Any spectroscopic probe should have acceptable signal-to-noise ratio to detect target signals in the presence of noise when they are applied to monitor biological systems. Indeed, challenges with sensitivity of designed spectroscopic probes for specific target is another challenge to detect small changes in biological systems. Beyond this, to collect accurate signals, biosensing and bioimaging, during the usage of spectroscopic probes, these probes should not be toxic because they may harm biological systems and increase the chance of false results. Specific and sensitive spectroscopic probes should have distinct and detectable spectral properties to identify the desired signals from noises, therefore accurate design of such chemical probes requires understanding the chemical nature of these probes and chemical substances that will be used to design spectroscopic probes. Therefore, the rational design of spectroscopic probes not only increases their effectiveness for bioanalysis but significantly also decreases the total cost and amount of chemicals required to develop and design these probes. Although designing spectroscopic probes for bioanalysis is a multi-step and sophisticated process due to the complexity of biological systems, understanding the essential aspects of target systems that should be targeted for spectroscopic probe design, along with the chemistry of probes can expedite the development of these probes and facilitate the understanding of the biochemistry, behaviour, and structure of biological systems.
Studies have shown that the challenges in spectroscopic probe design are mainly related to the application of these probes. In the case of spectroscopic probes for bioanalysis, sensitivity, high reaction rates, high resolution outputs, acceptable solubility, safety, simplified working mechanisms, and simplified optimization are critical factors that influence the quality of designed spectroscopic probes. Due to the complexity of biological systems, any spectroscopic probe that used for monitoring such systems should show high selectivity and sensitivity to produce high-quality results. However, achieving such effective and validate results requires applying rationale methods and appropriate chemical agents in designing spectroscopic probes for bio-analysis. The growing body of evidence suggests that coupling of advanced analytical methods and imaging processes can significantly increase the quality of spectroscopic probes outputs. In this regard, this Research Topic aims to cover all innovations and new trends in developing new methods to design spectroscopic probes with high selectivity and sensitivity, good solubility in different biosystems, and with fewer or no side effects for the target biosystems. We highly appreciate submissions that address current challenges in designing synthetic spectroscopic probes for bioanalysis as well as advanced computational studies on the mode of action of spectroscopic probes are also welcome to this Research Topic.
Define the scope of the Research Topic, listing specific themes you would like contributors to address. Indicate the types of manuscripts you are interested in.
We welcome Original Research, Review, Mini Review and Perspective articles on themes including:
• Tackling methodological barriers in developing spectroscopic probes
• Evaluation of stability, biocompatibility and the recognition ability of the spectroscopic probes
• New switches to produce spectroscopic signals
• Computational advancements in spectroscopic probe design, improving analytical performance of spectroscopic probes for bioanalysis
• Modifying chemical structure of current materials used as spectroscopic probes to improve their efficacy with applications in oncology, inflammation infection, cardio imaging, neuroinflammation and neuro-oncology.
Keywords: Spectroscopic probes, fluorescence probes, biological probes, probe design
Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.
