Third generation long-read sequencing such as Pacific Biosciences and Oxford Nanopore Technologies have made rapid advancement in terms of read length, throughput, yield and analysis methods. These technologies directly sequence single molecule of polynucleotides in real time and produce considerably longer ...
Third generation long-read sequencing such as Pacific Biosciences and Oxford Nanopore Technologies have made rapid advancement in terms of read length, throughput, yield and analysis methods. These technologies directly sequence single molecule of polynucleotides in real time and produce considerably longer reads than those from second generation sequencing platforms. In addition to generating longer reads, single molecule-based sequencing can also detect chemical modifications of the canonical bases including 5-methylcytosine (5mC). Investigators took advantage of these new capabilities to invent technologies that convert chromatin biological signals into base modifications. These technologies generate profiles of modifications to canonical DNA bases in multi-kilobase chromatin molecules. This special issue of Frontiers In Epigenetics and Epigenomics invites articles that describe technology innovations as well as analytical methods that apply long-read sequencing to investigate the epigenome.
Keywords:
long-read sequencing, DNA methylation, RNA modification, open chromatin, ONT, PacBio, single molecule real time sequencing, algorithm
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