Reactive Oxygen Species (ROS) Detection Methods in Biological System

Metabolic processes within the cellular system such as respiration and oxidative burst are known to be associated with the generation of reactive oxygen species (ROS). Exposure of biological system to any biotic and abiotic stress conditions are linked with the formation of ROS such as superoxide anion radical (O₂^•-); hydrogen peroxide (H₂O₂), hydroxyl radical (HO^•) and singlet oxygen (¹O₂). Reactive oxygen species possess a huge impact influencing the cellular function which at lower concentration are known to be involved in the regulation of cell signaling cascades however, its higher production leads to damage of biomolecules such as lipid, proteins and nucleic acid and are well documented to be associated with several biological dysfunction and human diseases. Reactive oxygen species has short life-time due to its high reactivity and thus its detection has always been a challenge. Different techniques have been applied for detection and quantification of varied ROS and their reactive intermediates during the past few decades either in vitro and in vivo studies.

The current Research Topic is aimed to bring about a collection of articles such as reviews and experimental contributions on the application of different spectroscopic, electrochemical and biochemical methods for the detection of ROS. We hope to bring a greater insight to the different approaches for the detection, identification, characterization and kinetics behavior on the generation of ROS. In this Research Topic, we welcome researchers to highlight methodologies on “ROS detection” in varied biological systems, and discuss other relevant issues.