About this Research Topic
As one of the most powerful research tools, gene editing has been widely used in plants for characterizing gene function and crop improvement, especially in recent years with the application of the CRISPR/Cas9 system. A number of genome editing tools in this field have been invented and a member of desired genes responsible for plant development, yield, metabolism regulation, disease resistance, biotic and abiotic stresses, et al., have also been analyzed by genome editing. These recent improvements of gene editing tools and approaches have enabled researchers to study gene function more effectively, precisely, and universally. Furthermore, applications of novel genome editing tools, like the transgene-free gene editing system, provide the possibility to narrow the gap between basic research and trait improvement of crops.
To further improve the efficiency, precision, and range of genome editing, we launch this Research Topic to present the recent progress in developing novel gene editing tools and their applications in laboratory studies and field practice. Minor and major methodological optimization of the zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeat (CRISPR)–Cas-associated system will be included. Any improved tools and protocols for more efficient genome editing, optimized approaches for editing event tests, novel gene editing tools for mitochondrial or chloroplast will bewelcomed. In particular, transgene-free gene editing systems like the regeneration system of protoplasts, nanomaterials and virus-based delivery of gene editing will be emphasized. The objectives of this Research Topic are also to present the applications of gene editing in new host plants, multiple gene knockout, and the generation of a mutant library.
We welcome submissions that cover (but are not limited to) the following:
• Minor and major optimization of tools and protocols resulting in increased efficiency of plant gene editing.
• Development of gene editing system in new host plants.
• Establishment of the transgene-free gene editing system by using protoplast regeneration, nanoparticle delivery system and/or virus-based vector.
• Application of gene editing in multiple gene knockout and construction of mutant library.
• Development of novel gene editing tools for mitochondrial or chloroplast.
• Development of Base editing and Prime editing system in eudicot plants.
All types of manuscripts, such as Original Research, Methods, Review, Mini Review, Perspective, Hypothesis and Theory, Brief Research Report, Opinion piece are welcomed.
To further improve the efficiency, precision, and range of genome editing, we launch this Research Topic to present the recent progress in developing novel gene editing tools and their applications in laboratory studies and field practice. Minor and major methodological optimization of the zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeat (CRISPR)–Cas-associated system will be included. Any improved tools and protocols for more efficient genome editing, optimized approaches for editing event tests, novel gene editing tools for mitochondrial or chloroplast will bewelcomed. In particular, transgene-free gene editing systems like the regeneration system of protoplasts, nanomaterials and virus-based delivery of gene editing will be emphasized. The objectives of this Research Topic are also to present the applications of gene editing in new host plants, multiple gene knockout, and the generation of a mutant library.
We welcome submissions that cover (but are not limited to) the following:
• Minor and major optimization of tools and protocols resulting in increased efficiency of plant gene editing.
• Development of gene editing system in new host plants.
• Establishment of the transgene-free gene editing system by using protoplast regeneration, nanoparticle delivery system and/or virus-based vector.
• Application of gene editing in multiple gene knockout and construction of mutant library.
• Development of novel gene editing tools for mitochondrial or chloroplast.
• Development of Base editing and Prime editing system in eudicot plants.
All types of manuscripts, such as Original Research, Methods, Review, Mini Review, Perspective, Hypothesis and Theory, Brief Research Report, Opinion piece are welcomed.
Keywords: Genome editing tools, trait improvement, Gene function, gene knockout, gene targeting
Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.
