About this Research Topic
With high-throughput-sequencing techniques becoming affordable to be used in plant health diagnostics, many labs started exploring the possibility to exploit its huge advantage, namely allowing untargeted virus discovery, eg. in view of resolving crop problems displaying an unknown disease etiology. This evolution not only required knowledge in the diverse wet lab techniques, yet also huge investments in data handling and standardizing bioinformatics pipelines. Gradually, HTS techniques not only gained interest in virus diagnostics but also for the detection and identification of nematodes, fungi, bacteria and even insects and mites (Piombo et al. 2021; Haegeman et al. 2021).
However, a lot of progress is still needed to achieve the ultimate goal; a fast, sensitive and reliable universal detection tool that allows simultaneous detection and identification of a wide range of plant pathogens and even pests. In addition to the strategies described above which are mostly used in routine plant health laboratories, methods based on the analysis of volatile compounds as biomarkers, remote sensing-based technologies (eg. plant-disease assessment based on hyperspectral datasets) in support of surveillance, spectroscopy-based methods (including visual -, near infrared -, shortwave infrared -, and thermal infrared wavelength based imaging), and finally biosensor development, such as biophotonics, are innovative methods that can strongly contribute to a more efficient plant disease assessment and monitoring.
In this Research Topic we aim to publish state of the art original research and reviews on the following, but not limited to, topics:
• The development and validation of generic and specific detection methods for plant pathogens
• Innovative technologies that assist detection and identification of plant pathogens in support of eg. early detection, warning systems, emerging problems and actual problems putting pressure on crop cultivation in horticulture and agriculture
• Integration of HTS technologies in plant-pathogen diagnostics in particular, and (regulatory) plant health in general
Keywords: innovative diagnostics, disease surveillance, point-of-care diagnostics, isothermal amplification, high-throughput sequencing, biosensors, bio-imaging, bio-informatics
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