About this Research Topic
On the other hand, Multiplex Immunohistochemistry/Immunofluorescence (mIHC/IF) has emerged to complement the conventional IHC as it can detect multiple biomarkers on a single tissue section, providing high-throughput multiplex staining and standardized quantitative analysis for highly reproducible, efficient, and cost-effective tissue testing. The ability to label multiple markers on a single section is of particular significance when studying samples taken from rare donors, where tissues may be of low availability. Having insufficient tissue for testing is a serious concern in the day-to-day clinical practice, especially during clinical trials and when using biopsy samples. For instance, the majority of biopsy samples have to be spared for molecular testing, which leaves limited samples for diagnosis by using conventional IHC. Cutting more consecutive sections may be impossible in this scenario, and this patient would lose the opportunity to benefit from the era of personalized medicine for immunotherapy.
In summary there is an urgent need for mIHC/IF for routine clinical practice in the era of cancer immunotherapy. Multiple biomarkers are needed to stratify patients, identifying responsive patients who can benefit, and sparing non-responders from unnecessary side-effects and cost. This Research Topic seeks to coordinate and discuss the promise and potential barriers for the clinical application of mIHC/IF from both scientific and technical perspectives in immuno-oncology and immuno-pathology. The topic will include both original articles and extensive reviews highlighting influential discoveries and applications of mIHC/IF in translational research. Topics may cover, but are not limited to, the following:
• Biomarkers Discovery by using mIHC/IF
• Spatial information of Tumor immune micro-environment by using mIHC/IF
• Immuno-phenotyping by using mIHC/IF
• Technical Reproducibility of mIHC/IF
• Comparison of mIHC/IF with other modality such as conventional IHC, RNAseq, NanoString
• High-dimensional analysis of mIHC/IF
• Analytic pipeline and algorithm of mIHC/IF
• Automation, standardization and upscaling the throughput of of mIHC/IF
• Comparison of two or more mIHC/IF techniques (e.g. DNA barcoding vs fluorescence vs metal-conjugation)
• Clinical application and translation of Biomarkers with mIHC/IF
Professor Bernard A Fox is the CEO of Ubivac, and Dr. Jaime Rodriguez is a full time employee of AstraZeneca. All other Topic Editors report no competing interests.
Keywords: multiplex IHC/IF, immunohistochemistry, clinical translation, pathology, immunotherapy
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