About this Research Topic
This Research Topic focuses on the recent development and application of novel genome engineering tools, particularly the CRISPR systems, for both model (i.e. E. coli and S. cerevisiae) and non-model (i.e. P. pastoris and Clostridium species) microorganisms .Therefore, the proposed Research Topic is aimed to collect original research articles, short communications, reviews, mini-reviews, methods and perspectives at the leading edge of the microbial genome engineering field. As previously discussed, it is hoped that further advancements in this field of research will enable:
1. Efficient assembly and editing of big gene clusters and discovery of novel secondary metabolites (natural products)
2. Assembly and integration of multi-gene biosynthetic pathways
3. Multiplex and combinatorial genome engineering tools for the optimization of microbial cell factories
4. Genome-scale engineering for functional genomics and evolution of complex phenotypes
Keywords: Genome Engineering, CRISPR, Microbial Biotechnology, Metabolic Engineering, Synthetic Biology
Important Note: All contributions to this Research Topic must be within the scope of the section and journal to which they are submitted, as defined in their mission statements. Frontiers reserves the right to guide an out-of-scope manuscript to a more suitable section or journal at any stage of peer review.