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REVIEW article
Front. Vet. Sci.
Sec. Animal Reproduction - Theriogenology
Volume 12 - 2025 | doi: 10.3389/fvets.2025.1554771
This article is part of the Research Topic Innovative Strategies for Improving Sperm Lifespan during Storage View all 3 articles
Extender Development for Optimal Cryopreservation of Buck Sperm to Increase Reproductive Efficiency of Goats
Provisionally accepted
Mustafa Bodu 1,2*
Mustafa Hitit 1* Olivia Chika Greenwood 1 Raheem Davian Murray 1
Erdogan Memili 1*- 1 Prairie View A&M University, Prairie View, Texas, United States
- 2 Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Selcuk University, Konya, Türkiye
Preservation of sperm significantly contributes to the advancement of assisted reproductive technologies, genetic conservation and improvement efforts, and precision breeding of livestock. This review distills knowledge from the existing information and emerging patterns in the field of buck sperm cryopreservation. The primary focus is on the challenges and opportunities associated with improving extender formulations and freezing techniques in order to enhance the vitality of sperm after thawing and to increase the potential for conception. This review assesses the efficacy and limitations of conventional extenders derived from egg yolk or soybean lecithin, and the adverse impacts of seminal plasma enzymes on sperm quality during the processes of chilling and cryopreservation. Significant progress has been made in the fields of molecular biology namely lipidomics, proteomics, metabolomics, DNA methylation providing valuable knowledge regarding the unique reactions of sperm to cryopreservation. The utilization of the "omics" technologies has shown intricate molecular transformation that occur in sperm during freezing and thawing. Moreover, detection of molecular biomarkers that indicate the quality of sperm and their ability to withstand freezing provides opportunities to choose the best sperm samples for cryopreservation. This, in turn, enhances the results of artificial insemination and genetic conservation endeavors. This review emphasizes the necessity for adopting a comprehensive approach that combines molecular and cellular knowledge with practical methods in the field of sperm cryopreservation to ensure production of goats as major food animals in the global scale.
Keywords: sperm, Cryopreservation, Extender, Antioxidants, omics, sustainable goat production, Food security
Received: 02 Jan 2025; Accepted: 27 Feb 2025.
Copyright: © 2025 Bodu, Hitit, Greenwood, Murray and Memili. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Mustafa Bodu, Prairie View A&M University, Prairie View, 77446, Texas, United States
Mustafa Hitit, Prairie View A&M University, Prairie View, 77446, Texas, United States
Erdogan Memili, Prairie View A&M University, Prairie View, 77446, Texas, United States
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.