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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Breeding
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1458294

Integrated analysis of BSA-seq and RNA-seq identified the candidate genes for seed weight in Brassica juncea

Provisionally accepted
Bin Yang Bin Yang 1Liu Yang Liu Yang 1*Lei Kang Lei Kang 1*Liang You Liang You 2*Chen Hao Chen Hao 1Huagui Xiao Huagui Xiao 3*Lunwen Qian Lunwen Qian 1*Yong Rao Yong Rao 3*Zhongsong Liu Zhongsong Liu 1*
  • 1 Hunan Agricultural University, Changsha, China
  • 2 Hunan University of Humanities, Science and Technology, Loudi, Hunan Province, China
  • 3 Guizhou Oil Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou Province, China

The final, formatted version of the article will be published soon.

    Brassica juncea is a major oilseed crop of Brassica. The seed weight is one of yield components in oilseed Brassica crops. To map the genes for seed weight, the parental and F2 extreme bulks derived were constructed from the cross between the heavy-seeded accession 7981 and the light-seeded one Sichuan yellow (SY) of B. juncea, and used in bulk segregant sequencing (BSA-seq). A total of thirty five SNPs were identified in thirty two genes located on chromosomes A02 and A10, while fifty eight InDels in fifty one genes located on A01, A03, A05, A07, A09, A10, B01, B02 and B04. Meanwhile, RNA-sequencing (RNA-seq) was performed for both parents at six seed development stages. A total of 7,679 differentially expressed genes were identified in developing seeds between the parents. Furthermore, integrated analysis of BSA-seq and RNA-seq data revealed a cluster of nine genes on chromosome A10 and one gene on chromosome A05 that are putative candidate genes controlling seed weight in B. juncea. This provides a foundation for research on seed weight in Brassica species.

    Keywords: Brassica juncea, Seed weight, BSA-seq, RNA-Seq, candidate genes

    Received: 02 Jul 2024; Accepted: 13 Nov 2024.

    Copyright: © 2024 Yang, Yang, Kang, You, Hao, Xiao, Qian, Rao and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Liu Yang, Hunan Agricultural University, Changsha, China
    Lei Kang, Hunan Agricultural University, Changsha, China
    Liang You, Hunan University of Humanities, Science and Technology, Loudi, 417000, Hunan Province, China
    Huagui Xiao, Guizhou Oil Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou Province, China
    Lunwen Qian, Hunan Agricultural University, Changsha, China
    Yong Rao, Guizhou Oil Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou Province, China
    Zhongsong Liu, Hunan Agricultural University, Changsha, China

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