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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Plant Breeding
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1443239

Identification of genetic loci for powdery mildew resistance in common wheat

Provisionally accepted
Xia Liu Xia Liu 1Xiaoqing Zhang Xiaoqing Zhang 2Peng Liu Peng Liu 3Menglin Lei Menglin Lei 4Hui Jin Hui Jin 5Yanzhen Wang Yanzhen Wang 4Yirong Jin Yirong Jin 3Guoqing Cui Guoqing Cui 4Zhixin Mu Zhixin Mu 4Jindong Liu Jindong Liu 6*Xiaoyun Jia Xiaoyun Jia 4
  • 1 College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, China
  • 2 National Agriculture Technology Extension Service Center (NATESC), Beijing, Beijing, China
  • 3 Dezhou Agriculture Science Academy, Dezhou, Shandong Province, China
  • 4 Shanxi Agricultural University, Jinzhong, Shanxi Province, China
  • 5 Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang Province, China
  • 6 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China

The final, formatted version of the article will be published soon.

    Powdery mildew (PM) poses an extreme threat to wheat yields and quality. In this study, 262 recombinant inbred lines (RILs) of Doumai and Shi 4185 cross were used to mapping PM resistance genes across four environments. High-density genetic linkage map of the Doumai/Shi 4185RIL population was constructed using the wheat Illumina iSelect 90K single-nucleotide polymorphism (SNP) array. Totally, four stable QTLs for PM resistance, QPm.caas-2AS, QPm.caas-4AS, QPm.caas-4BL, and QPm.caas-6BS were detected and explain 5.6-15.6% of the phenotypic variances. Doumai contributed all the resistance alleles of QPm.caas-2AS, QPm.caas-4AS, QPm.caas-4BL, and QPm.caas-6BS. Among these, QPm.caas-4AS and QPm.caas-6BS overlapped with the previously reported loci, whereas QPm.caas-2AS and QPm.caas-4BL are potentially novel. In addition, six high-confidence genes encoding the NBS-LRR-like resistance protein, disease resistance protein family and calcium/calmodulin-dependent serine/threonine-kinase were selected as the candidate genes for PM resistance. Three kompetitive allele specific PCR (KASP) markers, Kasp_PMR_2AS for QPm.caas-2AS, Kasp_PMR_4BL for QPm.caas-4BL and Kasp_PMR_6BS for QPm.caas-6BS were developed and their genetic effects were validated in a natural population including 100 cultivars. These findings will offer valuable QTLs and available KASP markers to enhance wheat marker-assisted breeding for PM resistance.

    Keywords: 90K SNP array, KASP, powdery mildew, Triticum aestivum, Resistance

    Received: 03 Jun 2024; Accepted: 02 Sep 2024.

    Copyright: © 2024 Liu, Zhang, Liu, Lei, Jin, Wang, Jin, Cui, Mu, Liu and Jia. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Jindong Liu, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.