AUTHOR=Liu Xia , Zhang Xiaoqing , Meng Xianghai , Liu Peng , Lei Menglin , Jin Hui , Wang Yanzhen , Jin Yirong , Cui Guoqing , Mu Zhixin , Liu Jindong , Jia Xiaoyun 

TITLE=Identification of genetic loci for powdery mildew resistance in common wheat

JOURNAL=Frontiers in Plant Science

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2024.1443239

DOI=10.3389/fpls.2024.1443239

ISSN=1664-462X

ABSTRACT=<p>Powdery mildew (PM) poses an extreme threat to wheat yields and quality. In this study, 262 recombinant inbred lines (RILs) of Doumai and Shi 4185 cross were used to map PM resistance genes across four environments. High-density genetic linkage map of the Doumai/Shi 4185 RIL population was constructed using the wheat Illumina iSelect 90K single-nucleotide polymorphism (SNP) array. In total, four stable quantitative trait loci (QTLs) for PM resistance, <italic>QPm.caas-2AS</italic>, <italic>QPm.caas-4AS</italic>, <italic>QPm.caas-4BL</italic>, and <italic>QPm.caas-6BS</italic>, were detected and explained 5.6%–15.6% of the phenotypic variances. Doumai contributed all the resistance alleles of <italic>QPm.caas-2AS</italic>, <italic>QPm.caas-4AS</italic>, <italic>QPm.caas-4BL</italic>, and <italic>QPm.caas-6BS</italic>. Among these, <italic>QPm.caas-4AS</italic> and <italic>QPm.caas-6BS</italic> overlapped with the previously reported loci, whereas <italic>QPm.caas-2AS</italic> and <italic>QPm.caas-4BL</italic> are potentially novel. In addition, six high-confidence genes encoding the NBS-LRR-like resistance protein, disease resistance protein family, and calcium/calmodulin-dependent serine/threonine-kinase were selected as the candidate genes for PM resistance. Three kompetitive allele-specific PCR (KASP) markers, <italic>Kasp_PMR_2AS</italic> for <italic>QPm.caas-2AS</italic>, <italic>Kasp_PMR_4BL</italic> for <italic>QPm.caas-4BL</italic>, and <italic>Kasp_PMR_6BS</italic> for <italic>QPm.caas-6BS</italic>, were developed, and their genetic effects were validated in a natural population including 100 cultivars. These findings will offer valuable QTLs and available KASP markers to enhance wheat marker-assisted breeding for PM resistance.</p>