Corrigendum: Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research

Burlacot, Adrien; Burlacot, François; Li-Beisson, Yonghua; Peltier, Gilles

doi:10.3389/fpls.2020.631667

CORRECTION article

Front. Plant Sci., 22 December 2020

Sec. Technical Advances in Plant Science

Volume 11 - 2020 | https://doi.org/10.3389/fpls.2020.631667

Corrigendum: Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research

This article is a correction to:

Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research
1. Read original article

$\nAdrien Burlacot&#x;$ Adrien Burlacot^†

François Burlacot^†

Yonghua Li-Beisson^†

Gilles Peltier^*^†

Aix Marseille Univ, Commissariat à l'énergie Atomique et aux énergies Alternatives (CEA), Centre National de la Recherche Scientifique (CNRS), Institut de Biosciences et Biotechnologies d'Aix- Marseille (BIAM), CEA Cadarache, Saint Paul-Lez-Durance, France

A Corrigendum on
Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research

by Burlacot, A., Burlacot, F., Li-Beisson, Y., and Peltier, G. (2020). Front. Plant Sci. 11:1302. doi: 10.3389/fpls.2020.01302

In the original article, in the paragraph on the “Assessment of Photosynthetic Oxygen Exchange,” we defined the O₂ uptake rate with a negative value when oxygen is consumed. Although it has been historically the first way to define it (Hoch and Kok, 1963), it makes more sense to use a positive value as adopted later by Radmer and Kok (1976) because a negative uptake would mean the usage of a double negative which implies production.

Therefore, Equations (1), (2), and (3) should be read;

\begin{array}{l} O_{2} U p t a k e_{} = {- v}_{_{}^{18} O_{2}} \times (1 + \frac{C_{_{}^{16} O_{2}} (t)}{C_{_{}^{18} O_{2}} (t)}) & (1) \end{array}

\begin{array}{l} {O_{2} E v o l u t i o n}_{} = v_{_{}^{16} O_{2}} - v_{_{}^{18} O_{2}} \times \frac{C_{_{}^{16} O_{2}} (t)}{C_{_{}^{18} O_{2}} (t)} & (2) \end{array}

\begin{array}{l} {N e t O_{2}}_{} = O_{2} E v o l u t i o n - O_{2} U p t a k e & (3) \end{array}

As a consequence, the plots shown on the original Figure 5 have been replaced by the attached Figure 5.

FIGURE 5

Figure 5. In vivo measurements of photosynthetic O₂ exchange in the presence of ¹⁸O-labeled O₂. (A) Schematic view of oxygen exchange illustrated in C. reinhardtii. While photosystem II (PSII) produces unlabeled O₂ from the photolysis of H₂O, oxygen uptake mechanisms consume both ¹⁸O-labeled and unlabeled O₂ (B) ¹⁶O₂ and ¹⁸O₂ concentrations measured in C. reinhardtii cells during dark–light transients. (C,D) Calculated cumulated O₂ exchanges (C) and the corresponding O₂ exchange rates (D) for the same experiment. Cells were grown photoautotrophically in air, centrifuged and resuspended in fresh medium at a concentration of 20 μg Chl ml⁻¹. Upon addition of 5 mM HCO $_{3}^{-}$ , ¹⁸O₂ was injected inside the cell suspension, and the reaction vessel was closed. After 5 min of dark adaptation, green light was turned on (500 μmol photon m⁻² s⁻¹) for 10 min. Levels of ¹⁶O₂ and ¹⁸O₂ were recorded at respective m/z = 32 and 36. O₂ Uptake (red), O₂ Evolution (blue), and Net O₂ production (black) were calculated as described; cumulated gas exchange were calculated by directly integrating obtained exchange rates. To limit noise on the exchange rates graphic, data shown in (D) are integrated with a sliding average of 30 s wide.

The authors would like to thank Dr. Duncan Fitzpatrick for highlighting this problem and for suggesting changes to increase clarity of the article.

The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated.

References

Hoch, G., Kok, B. (1963). A mass spectrometer inlet system for sampling gases dissolved in liquid phases. Arch. Biochem. Biophys. 101, 160–170. doi: 10.1016/0003-9861(63)90546-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Radmer, R. J., Kok, B. (1976). Photoreduction of O₂ pimes and replaces CO₂ assimilation. Plant Physiol. 58, 336–340. doi: 10.1104/pp.58.3.336

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: gas exchange, photosynthesis, carbonic anhydrase, CO₂ concentrating mechanism, O₂ evolution, H₂ production, microalgae, cyanobacteria

Citation: Burlacot A, Burlacot F, Li-Beisson Y and Peltier G (2020) Corrigendum: Membrane Inlet Mass Spectrometry: A Powerful Tool for Algal Research. Front. Plant Sci. 11:631667. doi: 10.3389/fpls.2020.631667

Received: 20 November 2020; Accepted: 03 December 2020;
Published: 22 December 2020.

Edited by:

Pietro Franceschi, Fondazione Edmund Mach, Italy

Reviewed by:

Giuseppe Pieraccini, University of Florence, Italy

Copyright © 2020 Burlacot, Burlacot, Li-Beisson and Peltier. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Gilles Peltier, Z2lsbGVzLnBlbHRpZXImI3gwMDA0MDtjZWEuZnI=

^†ORCID: Adrien Burlacot orcid.org/0000-0001-7434-6416
François Burlacot orcid.org/0000-0001-9783-6848
Yonghua Li-Beisson orcid.org/0000-0003-1064-1816
Gilles Peltier orcid.org/0000-0002-2226-3931

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