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ORIGINAL RESEARCH article
Front. Nutr.
Sec. Nutrition and Metabolism
Volume 12 - 2025 | doi: 10.3389/fnut.2025.1571473
This article is part of the Research Topic Prevention and Treatment of Metabolic Diseases Using Bioactive Metabolites of Herbal Medicines Also Used as Foods View all 10 articles
Delites™ Supplementation Prevents Metabolic Syndrome Onset and Modulates Gut Microbiome in Male Sprague Dawley Rats Fed on Cholesterol-and Fatenriched Diet: A Randomized Preclinical Trial Study
Provisionally accepted
Juan Leonardo 1
Robby Hertanto 2
Reggie Surya 3
Rony Abdi Syahputra 4*
Wardina Humayrah 5
Nindy Sabrina 5
Nurpudji Astuti Taslim 6
Trina Ekawati Tallei 7
Raymond Rubianto Tjandrawinata 8
Fahrul Nurkolis 10,11,9- 1 Citra Deli Kreasitama Ltd, Banten, Indonesia
- 2 Increase Laboratorium Indonesia, Tangerang, Indonesia
- 3 Bina Nusantara University, Jakarta, Jakarta, Indonesia
- 4 Faculty of Pharmacy, University of North Sumatra, Medan, North Sumatra, Indonesia
- 5 Sahid University, Jakarta, Jakarta SCR, Indonesia
- 6 Faculty of Medicine, Hasanuddin University, Makassar, South Sulawesi, Indonesia
- 7 Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado, North Sulawesi, Indonesia
- 8 Atma Jaya Catholic University of Indonesia, Jakarta, Jakarta, Indonesia
- 9 Medical Research Center of Indonesia, Surabaya, Indonesia
- 10 State Islamic University of Sunan Kalijaga (UIN Sunan Kalijaga Yogyakarta), Yogyakarta, West Java, Indonesia
- 11 Master of Basic Medical Science, Faculty of Medicine, Airlangga University, Surabaya, East Java, Indonesia
Background: Metabolic syndrome (MetS) is a global health concern, characterized by a combination of dyslipidemia, insulin resistance, obesity, and hypertension, significantly increasing the risk of type 2 diabetes mellitus (T2DM) and cardiovascular diseases. Gut microbiota plays a pivotal role in MetS pathophysiology, with dysbiosis exacerbating metabolic impairments. Delites™ , a supplement inspired by Traditional Chinese Medicine, has shown potential in modulating gut microbiota and mitigating MetS. Objectives: This study aimed to evaluate the effects of Delites™ supplementation on metabolic health and gut microbiota composition in male Sprague Dawley rats fed a cholesterol-and fat-enriched diet (CFED). Methods: A randomized preclinical trial was conducted on 32 rats divided into four groups: control-normal, CFED, CFED+low-dose Delites™ (54 mg/kg), and CFED+high-dose Delites™ (108 mg/kg). Parameters including lipid profiles, enzymatic activity, molecular biomarkers, and gut microbiota composition were analyzed. Results: Delites™ significantly improved lipid profiles, reduced inflammation (TNF-α), enhanced anti-inflammatory markers (IL-10), and increased energy metabolism regulator PGC-1α. Gut microbiota modulation showed increased beneficial genera ( , ) and reduced pathogenic , improving microbial diversity. Conclusions: Delites™ supplementation effectively mitigates MetS through metabolic and microbiota modulation. These findings highlight its potential for precision medicine approaches to combat metabolic disorders. Further research is needed to explore its long-term effects and translational relevance in humans.
Keywords: Traditional Chinese Medicine, diabetes, Obesity, microbiome, Drugs development, in vivo, Dyslipidemia, phytochemicals
Received: 05 Feb 2025; Accepted: 07 Apr 2025.
Copyright: © 2025 Leonardo, Hertanto, Surya, Syahputra, Humayrah, Sabrina, Taslim, Tallei, Tjandrawinata and Nurkolis. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Rony Abdi Syahputra, Faculty of Pharmacy, University of North Sumatra, Medan, 20155, North Sumatra, Indonesia
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.