95% of researchers rate our articles as excellent or good
Learn more about the work of our research integrity team to safeguard the quality of each article we publish.
Find out more
ORIGINAL RESEARCH article
Front. Nutr.
Sec. Nutrition and Metabolism
Volume 12 - 2025 | doi: 10.3389/fnut.2025.1549105
This article is part of the Research Topic Harnessing the Potential of Functional Foods Containing Bioactive Compounds: Implications for Health and Sustainability View all 3 articles
Hepatic Lipidomics Analysis Reveals the Anti-obesity Effects of Insoluble Dietary Fiber from Okara Combined with Intermittent Fasting Treatment in High-Fat Diet-Fed Mice
Provisionally accepted- 1 Department of Gastrointestinal Colorectal and Anal Surgery, China-Japan Union Hospital, Jilin University, Changchun, China
- 2 Jilin Agriculture University, Changchun, Jilin Province, China
- 3 China-Japan Union Hospital, Jilin University, Changchun, Jilin Province, China
- 4 Shandong Sinoglory Health Food Co., Ltd., Changchun, China
Emerging evidence has revealed that supplementation with insoluble dietary fiber (IDF) improves lipid metabolic disorders caused by a high-fat diet (HFD). Except for dietary supplementation, intermittent fasting (IF) has received widespread attention as a new dietary strategy against obesity. We hypothesized that IDF combined with IF treatment may synergistically alleviate HFD-induced lipid metabolic imbalance. Herein, this study integrated biochemical analysis with hepatic lipidomics to explore lipid biomarkers and potential mechanisms associated with the antiobesity effects of IDF combined with IF treatment. The results indicated that IDF combined with IF treatment improved metabolic parameters associated with the obesity phenotype. Lipidomics analysis revealed that IDF combined with IF treatment altered hepatic lipid metabolic patterns in HFD-fed mice.Moreover, 15 differentially regulated lipid species were identified as lipid biomarkers. Moreover, the expression of lipogenesis-, lipid oxidation-, and cholesterol metabolism-related genes was also regulated. Our results indicate that IDF combined with IF treatment ameliorates lipid metabolism disorders in HFD-fed mice by regulating hepatic lipid metabolites and related gene expression, providing evidence for its anti-obesity effects.
Keywords: Insoluble dietary fiber, Okara, high-fat diet, Anti-obesity, lipidomics
Received: 20 Dec 2024; Accepted: 31 Mar 2025.
Copyright: © 2025 Zhao, Wang, Li, Zhang, Yu and Yu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Wei Yu, China-Japan Union Hospital, Jilin University, Changchun, 130033, Jilin Province, China
Hansong Yu, Jilin Agriculture University, Changchun, 130118, Jilin Province, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.