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ORIGINAL RESEARCH article

Front. Nutr.
Sec. Food Chemistry
Volume 12 - 2025 | doi: 10.3389/fnut.2025.1542217
This article is part of the Research Topic Polyphenols and Betalains in Obesity and Metabolic Syndrome View all 3 articles

Ultrasound-Assisted Extraction of Polyphenols from Lonicera japonica Leaves and Their α-Glucosidase Inhibition

Provisionally accepted
Gang Li Gang Li 1Chunming Bao Chunming Bao 2Hao Zhang Hao Zhang 1Lu Bai Lu Bai 1*
  • 1 School of Food Science and Technology, Henan Institute of Science and Technology, Xinxiang, China
  • 2 Henan Institute of Metrology, Zhengzhou, China

The final, formatted version of the article will be published soon.

    The search for natural bioactive compounds that act as α-glucosidase inhibitors is a central focus in diabetes treatment research. This study utilized ultrasonic-assisted extraction to optimize the extraction of polyphenols from honeysuckle leaves through response surface methodology (RSM). Enzyme inhibition and fluorescence quenching experiments were conducted to examine the inhibitory activity and binding behavior of the extracted polyphenols. The results indicated that the optimal conditions for polyphenol extraction were as follows: ethanol concentration, 64%, liquid-solid ratio, 45 mL/g, ultrasonic power, 700 W, ultrasonic time, 6 min. Under these conditions, the yield of polyphenols from honeysuckle leaves was 9.16±0.19%, which closely aligns with the predicted value of 9.18%. The total phenolic content of the polyphenol extracts was 20.6±0.67%, with chlorogenic acid and luteoloside contents measuring 5.65±0.40% and 2.51±0.14%, respectively.The inhibitory effect of polyphenol extracts (IC50, 0.14±0.01 mg/mL) on α-glucosidase was better than that of chlorogenic acid (IC50, 0.55±0.02 mg/mL).Fluorescence quenching experiments indicated that the polyphenol extracts interact with α-glucosidase, resulting in alterations to the microenvironment of amino acid residues. This interaction can reduce the binding affinity between the substrate and α-glucosidase, thereby contributing to the objective of lowering postprandial hyperglycemia. Our research results can provide reference for the development and utilization of honeysuckle leaves.

    Keywords: Honeysuckle leaves, Polyphenol extracts, α-glucosidase, enzyme inhibition, fluorescence

    Received: 09 Dec 2024; Accepted: 15 Jan 2025.

    Copyright: © 2025 Li, Bao, Zhang and Bai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Lu Bai, School of Food Science and Technology, Henan Institute of Science and Technology, Xinxiang, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.