Thiamine, gastrointestinal beriberi and acetylcholine signaling

Research has highlighted numerous detrimental consequences of thiamine deficiency on digestive function. These range from impaired gastric and intestinal motility to aberrant changes in pancreatic exocrine function, gastric acidity and disturbances in gut barrier integrity and inflammation. Thiamine and its pharmacological forms, as a primary or adjunctive therapy, have been shown to improve symptoms such as nausea, constipation, dysphagia and intestinal dysmotility, in both humans and animals. This review aims to explore molecular mechanisms underlying the therapeutic action of thiamine in gastrointestinal dysfunction. Our analysis demonstrates that thiamine insufficiency restricted to the gastrointestinal system, i.e., lacking well-known symptoms of dry and wet beriberi, may arise through (i) a disbalance between the nutrient influx and efflux in the gastrointestinal system due to increased demands of thiamine by the organism; (ii) direct exposure of the gastrointestinal system to oral drugs and gut microbiome, targeting thiamine-dependent metabolism in the gastrointestinal system in the first line; (iii) the involvement of thiamine in acetylcholine (ACh) signaling and cholinergic activity in the enteric nervous system and non-neuronal cells of the gut and pancreas, employing both the coenzyme and non-coenzyme actions of thiamine. The coenzyme action relies on the requirement of the thiamine coenzyme form – thiamine diphosphate – for the production of energy and acetylcholine (ACh). The non-coenzyme action involves participation of thiamine and/or derivatives, including thiamine triphosphate, in the regulation of ACh synaptic function, consistent with the early data on thiamine as a co-mediator of ACh in neuromuscular synapses, and in allosteric action on metabolic enzymes. By examining the available evidence with a focus on the gastrointestinal system, we deepen the understanding of thiamine’s contribution to overall gastrointestinal health, highlighting important implications of thiamine-dependent mechanisms in functional gastrointestinal disorders.

1 Introduction

Gastrointestinal diseases are highly prevalent worldwide and account for a significant portion of global disease burden (1) and healthcare costs (2). Globally, a substantial proportion of these diseases are linked to infectious and transmissible causes, whereas in developed countries the vast majority are non-communicable and are rising in prevalence (3). The most common diagnoses in gastroenterology are a group of disorders known as Functional Gastrointestinal Disorders (FGIDs). With growing recognition in recent years of the nervous system’s involvement in such disorders, they are now broadly defined as disorders of gut-brain interaction (4). According to the Rome IV criteria, such disorders can involve any combination of motility disturbance, altered mucosal and immune function, dysbiosis of gut microbiota, visceral hypersensitivity, and altered central nervous system processing (5). The estimated prevalence is 10–40% (6), although the actual prevalence is unknown (7). A recent global study shows that 49% of females and 37% of males meet the diagnostic criteria for at least one FGID (7). The five most prevalent FGIDs are irritable bowel syndrome, functional dyspepsia, functional constipation, functional diarrhea, and functional bloating/abdominal distention. However, the FGID classification also includes a diverse range of other disorders, many of which exhibit overlapping clinical features. These include epigastric pain syndrome, functional dysphagia, heartburn, reflux hypersensitivity, belching disorder, bloating/distention, centrally mediated abdominal pain, fecal incontinence, and others.

The molecular and cellular mechanisms underpinning the pathophysiology of FGIDs are complex and have been reviewed elsewhere (5). Prominent features include epithelial barrier dysfunction, delayed or accelerated gastrointestinal transit due to abnormal function of smooth muscles, dysfunctional enteric nervous system and/or immunity, gut microbial dysbiosis, bile acid malabsorption, and alterations in the gut-brain axis (8–12). Therapeutic interventions include pharmacologic agents, dietary and lifestyle changes, probiotics, antibiotics, fecal microbial transplant, and stress management. Pharmacological treatments range from prokinetics and antispasmodics to centrally acting neuromodulators, albeit with varied success (13). As research continues to unearth a multitude of pathophysiological mechanisms involved in FGIDs, it is important to reveal specific primary causes. Similar symptoms may originate from different impairments, but therapies mitigating the primary impairment, rather than the convergent symptoms, are the most efficient ones (14).

Publications on FGIDs, which are ameliorated by administration of thiamine (vitamin B1) (15–23), suggest that thiamine insufficiency in the gastrointestinal system and/or its innervation may contribute causally to the development of these disorders, also in the absence of systemic thiamine deficiency. Our review aims at understanding the molecular mechanisms underlying this potential of thiamine to counteract many pathological alterations commonly observed in FGIDs. In addition to thiamine’s role as a precursor of an essential coenzyme in energy production from glucose oxidation (24), contribution to gastrointestinal function of the non-coenzyme action of thiamine (25, 26), presumably regulating not only the coenzyme-dependent pathway of acetylcholine (ACh) biosynthesis (27), but also ACh signaling at neuromuscular junctions (28) and in non-neuronal cells (29), is considered. Our focus on an intestine-expressed set of proteins which are well-known or suggested to be involved in transport, transformations and action of thiamine and its derivatives, underscores the significance of polar distribution of different thiamine transporters to organize the nutrient flux in enterocytes. We draw attention to various factors that may perturb enterocytic balance between the thiamine influx from the lumen and systemic delivery to other tissues, providing insights into the molecular mechanisms of local thiamine insufficiency in gastrointestinal system. Although this form of thiamine deficiency, which we call gastrointestinal beriberi, is initially limited to the gastrointestinal system, it mostly progresses to systemic thiamine deficiency, often triggered by stresses of different etiologies. That is why the distinction between specific gastrointestinal beriberi and the gastrointestinal manifestations of systemic beriberi resulting from gastrointestinal dysfunction remain unclear and are not well defined. However, in line with the existing classification of beriberi affecting nerves (dry beriberi) and cardiovascular system (wet beriberi), it is plausible to use the term gastrointestinal beriberi in an analogous, tissue-directed, way.

Our analysis of the gastrointestinal beriberi mechanisms employs, in particular, case studies of patients after bariatric surgery – a well-defined group, often developing systemic thiamine deficiency. Using available data on the time-dependent transcriptomic changes in enterocytes of these patients, we demonstrate long-term perturbations in their thiamine metabolism, which may be of diagnostic significance. Existence of genetic variants of thiamine-dependent proteins, insufficient molecular identification of these proteins, as well as the age- and sex-dependent differences in thiamine metabolism (30), may contribute to the observed variety of clinical manifestations of thiamine deficiency (31, 32). Clearly, this may also affect the outcomes of systemic and meta-analyses of thiamine’s effects in different populations and patient groups (32). As a result, our review calls upon more attention to personalized approaches in characterization of individual thiamine homeostasis, based on the knowledge of the thiamine-dependent proteins and their tissue-specific features and functions.

2 Thiamine (vitamin B1) and its metabolic significance

Thiamine is an essential water-soluble vitamin, naturally occurring in a wide variety of foods. Rich dietary sources include different types of meat, legumes and whole grains. In the human body, the major intracellular derivative of thiamine is its diphosphorylated form, thiamine diphosphate (ThDP). This essential coenzyme of ThDP-dependent enzymes is absolutely necessary not only for oxidative glucose metabolism, but also for coupling metabolic pathways of carbohydrates and amino acids, as well as for controlling de novo synthesis of major neurotransmitters, ACh and glutamate, from glucose. The control is determined by ThDP-dependent biosynthesis and/or distribution of the neurotransmitter precursors acetyl-CoA and 2-oxoglutarate, formed upon the oxidation of the glycolytic product pyruvate in the TCA cycle (Figure 1).

Figure 1

Figure 1. Coenzyme role of ThDP in metabolism of the gut cells. (A) Metabolic pathways involving enzymes that use the coenzyme derivative of thiamine, thiamine diphosphate (ThDP). (B) The relative abundance of mRNAs for the ThDP-dependent enzymes in different cells of intestine (colonocytes, enterocytes of ileum, enterocytes of jejunum) and in enterocyte organoids. The transcript signals for the genes of interest are normalized to the sum of the average mRNA signals of GAPDH, ACTB and TUBA1A as described earlier (149). The signals of these mRNAs for the three transcripts used for the normalization, are comparable, producing similar normalization ratios of the transcripts of interest across the different GEO datasets used. Transcriptomics data is taken from the GEO database. Identificators of the assessed experiments are: colonocytes experiments GSE13367 [Platform GPL570, (HG-U133_Plus_2) Affymetrix Human Genome U133 Plus 2.0 Array, 10 datasets: GSM337520, GSM337526, GSM337529, GSM337530, GSM337532, GSM337533, GSM337537, GSM337539, GSM337540, GSM337544] and GSE30292 [Platform GPL570, (HG-U133_Plus_2) Affymetrix Human Genome U133 Plus 2.0 Array, 3 datasets: GSM750882, GSM750883, GSM750884]; jejunum enterocytes experiments GSE214758 [Platform GPL20795, HiSeq X Ten (Homo sapiens), 9 datasets: GSM6615629, GSM6615631, GSM6615633, GSM6615637, GSM6615641, GSM6615643, GSM6615645, GSM6615647, GSM6615649], GSE113819 {Platform GPL17586 (HTA-2_0) Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version], 5 datasets: GSM3120595, GSM3120597, GSM3120599, GSM3120601, GSM3120603} and GSE30292 [Platform GPL570, (HG-U133_Plus_2) Affymetrix Human Genome U133 Plus 2.0 Array, 2 datasets: GSM750891, GSM750892]; ileum enterocytes experiment GSE30292 [Platform GPL570, (HG-U133_Plus_2) Affymetrix Human Genome U133 Plus 2.0 Array, 3 datasets: SM750888, GSM750889, GSM750890]; enterocyte organoid experiment GSE242765 [Platform GPL18573, Illumina NextSeq 500 (Homo sapiens), 1 dataset GSM7770156]. Normalized mRNA levels from all the datasets for the same cell type are averaged, and the data are shown as mean ± SEM.

The mammalian ThDP-dependent enzymes (24) include cytosolic transketolase (TKT) participating in the pentose phosphate pathway; the mitochondrial multienzyme complexes of 2-oxo acids, i.e., of pyruvate dehydrogenase (PDH), 2-oxoglutarate dehydrogenase (OGDH), 2-oxoadipate dehydrogenase (OADH) and branched chain 2-oxo acid dehydrogenase (BCODH), linked to amino acids through their transamination to the 2-oxo acids; and peroxisomal 2-hydroxy acyl-CoA lyase (HACL) participating in the α-oxidation of long and very long chain 3-methyl or 2-hydroxy even-chain fatty acids (33).

TKT, as part of the cytosolic pentose phosphate pathway, along with the mitochondrial multienzyme complexes of pyruvate (PDHC) and 2-oxoglutarate dehydrogenases (OGDHC), are essential for energy production from glucose oxidation. The oxidation-generated reducing equivalents accumulate as cytosolic NADPH produced by the pentose phosphate pathway, and mitochondrial NADH, produced by 2-oxo acid dehydrogenases. In addition, the function of TKT in the pentose phosphate pathway supports the generation of phosphoribose for nucleic acid synthesis. PDHC links cytosolic glycolysis to the mitochondrial TCA cycle by oxidizing pyruvate, the end product of glycolysis. The PDHC-catalyzed reaction generates not only NADH, which is oxidized in the mitochondrial respiratory chain, but also acetyl-CoA, which feeds into the TCA cycle and is a precursor of acetylcholine (ACh) in cholinergic cells. OGDHC catalyzes the rate-limiting step of the TCA cycle. The substrate of the OGDHC reaction, 2-oxoglutarate, is a precursor for glutamate biosynthesis from glucose. The OGDHC reaction product succinyl-CoA provides for the only mitochondrial phosphorylation reaction of ADP to ATP, at the substrate-level, i.e., phosphorylation beyond mitochondrial respiratory chain.

In addition to the ubiquitous ThDP-dependent enzymes, enterocytes express a number of the ThDP-dependent isoenzymes, i.e., the enzymes with similar catalytic functions, encoded by separate genes. These include transketolase-like 1 and 2 proteins, TKTL1 and TKTL2, as well as the ubiquitous (PDHA1) and testis-specific (PDHA2) isoenzymes of the α-subunit of PDH (PDHA) (Figure 1B). Deciphering physiological roles of isoenzymes is often challenging. Inactivation of TKTL1 aggravates colitis in a murine knockout model (34, 35). Isoenzymes of HACL1 and HACL2 are shown to provide α-oxidation of 3-methyl and 2-hydroxy long chain fatty acids, respectively, with HACL2 playing an important role in ceramide formation in the stomach (33). Isoenzymes of OGDH are the brain-specific OGDH-like protein (OGDHL) and OADH, encoded by OGDHL and DHTKD1 genes, correspondingly (Figure 1). OGDH and OGDHL differ in their regulatory properties, including 2-oxoglutarate saturation (36). OGDH(L) and 2-oxoadipate dehydrogenase (OADH) have different substrate specificity, preferring 2-oxoglutarate and 2-oxoadipate, correspondingly (37, 38). The OGDH isoenzymes encoded by OGDHL and DHTKD1 genes, link eosinophilic esophagitis to mitochondrial dysfunction (39).

Functional significance of the ThDP-dependent enzyme isoforms, which are the enzyme variants arising from alternative splicing of transcripts from a single gene, or through post-translational modifications, is studied even less than that of isoenzymes. An exception is the established regulatory significance of the OGDH splice variants lacking Ca²⁺-dependent regulation. Unlike skeletal muscle and heart, which predominantly express Ca²⁺-sensitive isoforms, other tissues, in particular pancreatic islets, have significant expression of Ca²⁺-independent OGDH isoforms, which are thought to be involved in the distribution of 2-oxoglutarate flux to oxidation in the TCA cycle and glutamate biosynthesis (40).

As mentioned above, the coenzyme role of thiamine is important for metabolism of α-amino acids, as they are transaminated to 2-oxo acids. In addition to PDHC and OGDH(L)C, this action of thiamine is mediated by two other ThDP-dependent multienzyme complexes, i.e., those of branched chain 2-oxo acid dehydrogenase (BCODH) and OADH, functioning in the pathways of degradation of branched-chain α-amino acids, and lysine and tryptophan, respectively (Figure 1). The corresponding acyl-CoAs generated by these complexes undergo additional transformations before entering the TCA cycle in the form of acetyl- or succinyl-CoA. As a result, catalytic function of the multienzyme complexes of 2-oxo acid dehydrogenases may provide energy from oxidation of both carbohydrates and amino acids. Thiamine-dependent metabolic regulation is important for optimizing the energy source: by increasing efficiency of glucose oxidation, thiamine prevents excessive degradation of amino acids (41).

In recent years, the role of different acyl-CoAs in the posttranslational acylation of protein lysine residues has acquired increasing attention (42). Histone acylations are considered to be especially important for intestinal epithelium adaptations to environmental signals (43). Remarkably, nuclear localization of the 2-oxo acid dehydrogenase complexes has recently been discovered, in addition to their traditional mitochondrial localization (44–47). This finding is in good accordance with independent research that acylations of both metabolic proteins and histones depend on the function of 2-oxo acid dehydrogenase complexes (48, 49). The changes in histone acylation, mediated by ThDP-dependent 2-oxo acid dehydrogenase complexes, provide a mechanism for thiamine-dependent transcriptional regulation.

Positioning of ThDP-dependent 2-oxo acid dehydrogenase complexes at the intersections of major pathways of central metabolism and transcriptional regulation endows these systems with a functional role of “signaling hubs,” regulating multiple cellular processes, such as intracellular redox status, growth, protein signaling, and calcium homeostasis (50–52).

Expression of the considered ThDP-dependent enzymes and isoenzymes in enterocytes (Figure 1B) underscores both the common and isoenzyme-specific dependence of gastrointestinal metabolism on thiamine.

3 Gastrointestinal beriberi

As animals do not synthesize thiamine, their thiamine need is satisfied by dietary intake and biosynthesis by gut microbiota. Thus, gastrointestinal dysfunction impairing the absorption of nutrients inevitably results in insufficient organismal thiamine levels, i.e., thiamine deficiency (TD). Indeed, the most well-known TD state is Wernicke-Korsakoff syndrome, first described by Wernicke in a female whose digestive tract was severely damaged by sulfuric acid (53). On the other hand, gastrointestinal disturbances are critical early indicators of severe thiamine deficiency causing pediatric Wernicke Encephalopathy (54).

An estimated 2–3% of the body’s thiamine may originate from microbial synthesis within the colon, although its precise nutritional significance remains to be fully understood. Studies on the gut microbiome have identified distinct genera of bacteria capable of synthesizing thiamine, including Lactobacillus, Bifidobacterium, Escherichia coli, Enterococcus, and Clostridium (55, 56). Furthermore, high expression of genes involved in thiamine biosynthesis and transport are prevalent in Prevotella, Desulfovibrio (57), and Bacteroides (58). On the other hand, the microbiome houses many genera of bacteria that fail to grow in the absence of thiamine (59), and depend on its external sources (60). The abundance of one such family, Ruminococcaceae, is positively correlated with thiamine intake in humans. Dietary thiamine restriction in rodents also decreases relative abundance of this family, which is accompanied by reduced fecal butyrate concentrations (61). ThDP is used as a cofactor for the microbial enzyme pyruvate-ferredoxin oxidoreductase, which catalyzes the conversion of pyruvate to acetyl-CoA in the pathway for butyrate synthesis (56). Hence, bacteria require thiamine for production of short-chain fatty acids, which play important anti-inflammatory and signaling roles in the gut. It is therefore not surprising that thiamine from dietary sources may alter the composition of microbes in the gut, and a deficit of thiamine may result in bacterial dysbiosis (58, 59).

Remarkably, in a trial using high-dose thiamine for fatigue related to inflammatory bowel disease (IBD), the relative abundances of Faecalibacterium prausnitzii, a member of the Ruminococcacea family, and Roseburia hominis, a member of the Lachnospiraceae family, inversely correlate with fatigue severity both pre- and post-treatment with thiamine (62).

When TD becomes severe and chronic, it is known as beriberi. Dry beriberi affects the central nervous system (CNS) and peripheral nerves, whereas wet beriberi involves the cardiovascular system. In 2004, Dr. Michael Donnino introduced the term “gastrointestinal beriberi” (63) to describe a distinct clinical entity caused by TD in the gastrointestinal system. It is broadly defined as a combination of several possible symptoms: anorexia (lack of appetite), nausea, unexplained vomiting, abdominal distention, constipation, reflux and epigastric pain, and occasionally intestinal paralysis. Since its original definition in the medical literature, multiple case reports have been published by independent researchers (Table 1). Although manifestations of neurological dysfunction often point to TD and may sometimes accompany gastrointestinal beriberi (17, 64), it has been also noted that chronic mild deficiency may present with gastrointestinal symptoms under entirely normal neurological exams in well-nourished, non-alcoholic patients (65–68). In one of the early reports cited by Donnino (69), severe manifestations of gastrointestinal beriberi are also preceded in time by much milder symptoms. These include abdominal distension, belching, and alternating constipation and diarrhea. Furthermore, early into the study, all participants exhibited achlorhydria or hypochlorhydria, delayed gastric emptying, and reduced intestinal motility. Similarly, early gastrointestinal symptoms - such as a full sensation in the epigastrium, gastric reflux, hypochlorhydria, impaired gastric and intestinal motility, and constipation - are reported by Shimanoza and Katsura in 30–50% of patients with TD (70). In another study, thiamine therapy leads to the disappearance of both gastrointestinal (dysphagia) and CNS/cardiovascular (dyspnea and blurred vision) symptoms. However, after thiamine is discontinued, dysphagia re-appears at the time when other symptoms are not observed (71). One case report describes the onset of dysphagia and gastroparesis in a patient with Crohn’s disease 2 months before developing Wernicke encephalopathy, which resolved with high dose thiamine (72). A recent analysis (19) has found gastrointestinal symptoms in 46 out of 52 patients with diagnosed TD. In some cases, symptoms precede the classical neurological signs by up to several months, suggesting that digestive dysfunction may, in fact, be an early indicator of TD before progressing to other bodily systems. Dysphagia, or esophageal dysmotility, is common after bariatric surgery, known to be associated with TD, and is restored following thiamine administration (73, 74). A high prevalence of constipation and gastrointestinal paralysis is also associated with TD (70). Numerous reports demonstrate significant impairments in intestinal motility (small and large) in TD subjects, which normalizes with thiamine repletion (18, 75–78). Dietary thiamine intake is inversely associated with constipation (79). Abnormal gastric acidity is reported to be one of the most common and early signs of TD (70). In this study, four patterns of acid secretion are identified in response to thiamine administration: (i) Hypoacidity which gradually improves with thiamine repletion; (ii) Hypoacidity which shifts to high acidity in the recovery phase after thiamine administration, followed by eventual normalization; (iii) Hyperacidity in the early stage of TD, followed by normalization with thiamine therapy; (iv) Hyperacidity, followed by low acidity during the recovery phase after thiamine administration, which normalizes later. More recently, aberrant changes in gastric output have been demonstrated in TD. Achlorhydria is found in both human (80–83) and animal (84) studies. TD is also known to induce gastric ulceration (85). Epigastric pain, early satiety, and gastroesophageal reflux are common initial symptoms of TD and gastrointestinal beriberi (19, 70). Based on modern diagnostic criteria, this combination of symptoms could now be broadly classified as functional dyspepsia, which is often associated with gastric hypoacidity (86). Thiamine in conjunction with other therapies has been used to successfully treat functional dyspepsia (87).

Table 1

Table 1. Therapeutic action of thiamine administration in human and animal studies of FGIDs.

Thus, a wide range of gastrointestinal disorders respond positively to thiamine administration. The therapeutic action of thiamine administration in human and animal studies are summarized in Table 1.

An important factor for digestion and overall gastrointestinal health is pancreatic function (88). Of note, the pancreas has a high thiamine content (89) which it maintains through constant uptake from circulation (90). TD is associated with a dramatic reduction of pancreatic thiamine content by up to 75% (89). Depletion of pancreatic thiamine can result in oxidative stress (91), which is considered to be a driving factor in development of pancreatitis. Furthermore, some researchers speculate that thiamine depletion in the pancreas may be a necessary antecedent for pancreatic inflammation (91). Indeed, chronic alcoholism and nicotine consumption are independent risk factors for pancreatitis, and both inhibit pancreatic thiamine uptake via downregulation of thiamine transporters (90). A Spanish review published in 1944 reports that hyposecretion of pancreatic enzymes is a common feature of TD (92), and administration of thiamine in children could increase the release of the pancreatic enzymes trypsin, amylase and lipase (93). Animal research also highlights substantial abnormalities in pancreatic function during TD, evidenced by a reduction in stored pancreatic protein and digestive enzyme content, contrasted by an abnormally large enzyme secretion (94). Acute pancreatitis and encephalopathy are recently reported as a consequence of TD and successfully treated with thiamine administration (95).

As a result, thiamine is known to positively influence perturbed gastrointestinal function. In many cases, this positive influence occurs when CNS symptoms of TD are absent, pointing to the gastrointestinal system as the primary site of TD. The findings suggest that TD restricted to the gastrointestinal tract may occur, while other TD-sensitive systems, such as the neurological and cardiovascular systems, do not display their specific symptoms. That said, chronic impairment of thiamine availability in the gastrointestinal system should also reduce resilience of other body systems. Obvious neurological and/or cardiovascular symptoms may represent a culmination of gastrointestinal beriberi, probably triggered by challenges that increase metabolic demands or decrease intracellular transport of thiamin, such as stress, infection, trauma, drug administration. For instance, a case study describes persistent gastrointestinal beriberi followed by the onset of neurological symptoms in a male patient after one session of heavy drinking (17). Metformin, a widely used antidiabetics entering cells through the thiamine transporters, is known to reduce intestinal thiamine content in mice (96). Thus, different comorbidities may transition a vulnerable state of gastrointestinal beriberi—where TD is confined to the gastrointestinal tract, but is not obviously affecting other body systems—into dry or wet beriberi, characterized by specific symptomatic manifestations where potentially life-threatening situations promote the diagnosis of TD.

In conclusion, TD limited to the gastrointestinal system may be an overlooked and underdiagnosed cause of the increasingly common gastrointestinal disorders encountered in modern medical settings. Left unattended, it may progress to wet or dry beriberi, most often observed as Wernicke encephalopathy. However, how is it possible that the gastrointestinal system—which, unlike other systems, is directly exposed to nutrients—suffers from this nutrient deficiency more than the rest of the body? The following sections examine the available evidence on molecular mechanisms of thiamine transport, metabolism, and function in the gastrointestinal system, offering insights for the interpretation of medical studies on thiamine-responsive gastrointestinal disorders that highlight mechanistic connections between common disorders of gastrointestinal tract and TD.

4 Thiamine metabolism in intestinal cells

4.1 Thiamine transport in brush border and basolateral membranes of enterocytes

Due to its hydrophilic nature, thiamine is not membrane-permeable, with its entry into the cell depending on membrane proteins dedicated to thiamine transport. Enterocytic absorption of dietary thiamine from the lumen and further transport of thiamine from the intestine to the blood occur via active transport through a number of transporters (Figure 2A). Thiamine transport capacity in human intestinal biopsy samples is highest in the duodenum, followed by the colon and stomach (97). Although THTR1 and THTR2 transporters, encoded by the SLC19A2 and SLC19A3 genes, respectively, are the most widely known and well-characterized, a number of newly characterized transporters of thiamine and its phosphates have been added to the list in recent decades. Figure 2 shows those expressed in intestine (A) along with their relative expression in enterocytes, their organoids and colonocytes (B).

Figure 2

Figure 2. Transporters of thiamine and its derivatives in the gut cells. (A) Schematic presentation of the transport processes through transporters expressed in the gut cells. (B) The relative abundance of mRNAs for the transporters of thiamine and its derivatives in different intestinal cells (colonocytes, enterocytes of ileum, enterocytes of jejunum) and in enterocyte organoids. The normalized mRNA signals are calculated as described in the legend to Figure 1 using the same datasets. The data are shown as mean ± SEM.

Thiamine transporter SLC35F3 has been identified in studies investigating its variant, which is associated with human hypertension and lower levels of erythrocytic thiamine; expression of the protein in Escherichia coli increases thiamine transport (98). Thiamine is also transported through OCT1 (SLC22A1) (96) and OCT2 (SLC22A2) (99). Although the substrate specificity of other OCT family members is insufficiently characterized, their principal ability to transport organic cations justifies inclusion of SLC22A3–5 in Figure 2.

Intestinal alkaline phosphatase (ALPI, Figure 2) plays an essential role in the absorption of dietary B vitamins including thiamine (100). This enzyme not only exhibits phosphatase activity, dephosphorylating thiamine phosphates in the lumen, but may also transphosphorylate extracellular thiamine to intracellular thiamine monophosphate (ThMP), at the expense of intracellular phosphate donors such as beta-glycerophosphate or creatine phosphate (101). Transporters of MATE (multidrug and toxin extrusion) family (SLC47A) extrude thiamine in exchange for a proton; SLC47A1 and SLC47A2-K are characterized by a Km for thiamine in micromolar range (102), supporting the physiological relevance of thiamine extrusion by these transporters (Figure 2A).

Differences in the net intestinal transport of thiamine have been studied by Rindi and co-workers using basolateral and brush border membrane vesicles (100, 103, 104). However, apart from the SLC19A transporter family, little is known about the polar distribution of transporters in enterocytes and colonocytes. The gut cells have an apical, or brush border, membrane facing the lumen, and a basolateral membrane facing the circulatory system. This polarity is in accord with the dual function of intestinal epithelium: the same cells absorb nutrients from the lumen through the brush border membrane and deliver them to the blood through the basolateral membrane (Figure 2A). Available data on the polarity are taken into account in Figure 2A. These refer to the excretory function of MATE transporters (102, 105, 106) and the identification of SLC19A2 on both membrane types in epithelial cells (107). In contrast, the SLC19A3 protein is shown to localize specifically to the apical (brush border) membrane (108). This transporter is well-characterized in terms of its structure and function, with a number of drugs decreasing thiamine transport through SLC19A3 (109). With the brush border membrane directly exposed to drugs inhibiting thiamine influx through SLC19A3, and nutrient efflux through the basolateral membrane to satisfy permanent systemic demands for thiamine, the steady-state concentration of thiamine in the gut epithelium may be decreased. The disbalance between the inhibited enterocytic influx and unchanged or increased systemic demand may cause specific vulnerability of the gut epithelium to toxic effects of the thiamine-competitive drugs targeting SLC19A3. As a result, gastrointestinal TD may develop and exist as a steady-state, providing other tissues with thiamine levels sufficient for normal conditions, but inadequate for metabolic challenges.

As luminal ThDP from the diet is supposed to be hydrolyzed by ALPI, while ThDP is known to be produced inside the cell, the role of SLC44A4 in transporting extracellular ThDP has been enigmatic. Single nucleotide polymorphisms in SLC44A4 are key risk factors for ulcerative colitis in a collection of different studies (110–113). In view of the high expression of this transporter in colon, it has been proposed to participate in absorption of the microbiota-generated ThDP (25). However, this view leaves unanswered the question why microbes would extrude ThDP to the lumen. Besides, the transporter is also expressed in other tissues, and independent data (114–116) show a less drastic difference between human colonocytes and enterocytes in its expression (Figure 2B), compared to the initial finding of a 10-fold higher expression in the colon relative to other regions of gastrointestinal tract in humans (117). On the other hand, according to recent structure–function characterization of reduced folate transport through SLC19A1, ThDP is the best substrate to be exchanged for folate (118). The participation of ThDP in cellular folate absorption (Figure 2A) endows SLC44A4 with a physiologically relevant function to return ThDP that is extruded in exchange for folate, back to the cell (Figure 2A). Employment of the ThDP-folate exchange by microbes may explain the high SLC44A4 expression in the colon. Yet, the expression of this transporter in different parts of the gut (Figure 2B) does not exclude its possible participation in the gut’s absorption of ThDP from the diet, especially given the presence of other folate transporters on the brush border membrane of enterocytes, i.e., those encoded by SLC46A1, FOLR1, and FOLR2 (119).

Interestingly, males display lower plasma folate levels than females (120–122), and only males demonstrate a positive correlation between the folate deficiency risk scores calculated from the polymorphisms in the folate pathway genes SLC19A1 and MTHFR (123). At the same time, it is known that thiamine intake is higher in males vs. females (124). The opposite sex-dependent differences in the levels of folate and thiamine highlight the physiological significance of the folate/ThDP exchange through SLC19A1. This is further supported by a study of the SLC19A1 variant rs1051266:G (125). In model experiments employing HEK293 cells in a thiamine-deficient medium, the SLC19A1 variant rs1051266:G exhibits a strong decrease in ThDP efflux compared to the canonic SLC19A1 sequence rs1051266:A. Association of the SLC19A1 variant rs1051266:G with Wernicke-Korsakoff encephalopathy (125), a condition known to develop due to the thiamine deficiency in the brain, underscores the role of SLC19A1 in delivering not only folate but also thiamine to CNS (28). Both SLC19A1 and SLC19A2 deliver vitamins to systemic tissues (119), correspondent to their locations in the basolateral membrane (Figure 2). ThDP transport through the folate transporter encoded by SLC19A1 is further supported by cases of thiamine-responsive dysphagia that have been observed at normal laboratory values of blood thiamine, but increased serum folate levels (71). The blood transports thiamine or its monophosphate (ThMP), both of which also penetrate the blood–brain barrier (26, 126) through saturable transporters (127).

4.2 Interconversions of thiamine and its natural derivatives in intestinal cells

To perform its coenzyme function (Figure 1A), thiamine or ThMP entering the cells (Figure 2A) must be transformed into the coenzyme form, ThDP. Animals synthesize ThDP by diphosphorylation of thiamine in the thiamine diphosphokinase (or thiamine pyrophosphokinase, TPK)-catalyzed reaction (Figure 3A). Another well-characterized protein of thiamine metabolism is thiamine triphosphatase, encoded by ThTPA gene, which hydrolyzes thiamine triphosphate (ThTP) to ThDP (128, 129). The enzymes catalyzing other transformations of thiamine derivatives are not unambiguously identified. Given the poorly characterized specificity of enzymes involved in thiamine transformation and the often unknown roles of genomics-identified isoenzymes, all human isoenzymes are included in Figure 3 when there is evidence that at least one isoenzyme can catalyze the reactions with thiamine or its derivatives. In doing this, we would like to draw attention to possible roles of the isoenzymes, as shown in Figure 3, in thiamine metabolism. This may further help decipher their physiological significance, e.g., when the data on phenotypes of the mutated isoenzymes in humans may appear.

Figure 3

Figure 3. Enzymatic transformations of thiamine and its derivatives in the gut cells. (A) Schematic presentation of the reactions and available information on their catalysts. (B) The relative abundance of mRNAs for the enzymes of thiamine metabolism in different intestinal cells (colonocytes, enterocytes of ileum, enterocytes of jejunum) and in enterocyte organoids. The normalized mRNA signals are calculated as described in the legend to Figure 1 using the same datasets. The data are shown as mean ± SEM.

ThMP kinase, which produces ThDP through ThMP phosphorylation and is known in thiamine-synthesizing organisms, has not been identified in animals. Accordingly, to be transformed into ThDP by TPK, intracellular ThMP should first be dephosphorylated. ThMP hydrolysis is known to be catalyzed by prostatic acid phosphatase ACP3, which is localized to plasmatic membrane and linked to the antinociceptive action of thiamine and its derivatives (130–132). Cells of the gut express all isoenzymes of acid phosphatase (ACP), i.e., ACP 1–7 (Figure 3B). According to the UniProt database, these isoenzymes have diverse locations and substrate specificities, acting on protein phosphotyrosine residues and a number of alkyl, aryl and acyl orthophosphates of low molecular mass. ACP1 has cytosolic location, whereas ACP2 and ACP5 are lysosomal enzymes. ACP4 is a transmembrane protein with 50% homology to the prostatic and lysosomal acid phosphatases, highly expressed in the testis and involved in mineralization of tooth enamel (133, 134). ACP6 is mitochondrial, supposed to participate in lipid metabolism through its characterized activity of hydrolyzing lysophosphatidic acid (135). Based on similarity to ACP5, ACP7 is predicted to be a putative tartrate-resistant phosphatase, a member of purple acid phosphatase family of metallophosphoesterase superfamily. The most probable locations of ACP7 are supposed to be extracellular space and cytosol. Studies on substrate specificities of ACP isoenzymes 1–7 are scarce and fragmentary, not enabling a conclusion on their specific catalysis of ThMP hydrolysis. Additionally, the substrate specificity may differ in the isoforms of each gene product, arising due to posttranscriptional (alternative splicing) or posttranslational modifications. Hence, Figure 3 shows all of the ACP isoenzymes expressed in the gut as potential catalysts of ThMP hydrolysis.

A deficiency of acid phosphatase activity in the lysosomal fraction, presumably due to impairment of the ACP2 protein, manifests as intermittent vomiting, hypotonia, lethargy, opisthotonos, terminal bleeding and death in early infancy (136). Many of these symptoms are also present in the thiamine deficiency states (137), supporting involvement of ACP2 in thiamine metabolism. Pathogenic mutation of the tartrate-resistant acid phosphatase ACP5 leads to a strong predisposition to autoimmune diseases, associated with the accumulation of phosphorylated osteopontin, involved in immune regulation and in bone resorption (138). As shown in the next sections, thiamine is tightly linked to immunity. Furthermore, both ACP5 (30) and a ThMP analog benfotiamine (139) are involved with Akt signaling. These lines of evidence favor catalytic activity of ACP5 in ThMP hydrolysis.

Thiamine-specific phosphatases of the bovine brain synaptosomes have been studied by binding them to a thiamine-modified affine sorbent, followed by MS identification of the eluted proteins. Only bacterial paralogues of mammalian proteins with phosphatase activities are identified by the procedure, probably due to poor coverage of the bovine genome. According to the sequence and structural alignment of these identified phosphatases, an apyrase encoded by the ENTPD2 gene, that is expressed in different tissues, including colon and small intestine, may possess the ThDP phosphatase activity in mammals (140). Apyrases, or ectonucleoside diphosphohydrolases, may catalyze hydrolysis of triphosphonucleotides to their monophosphates. Furthermore, these membrane-bound and soluble hydrolases may also act as monophosphatases of the diphosphonucleotides, catalyzing the hydrolysis of ThDP to ThMP (Figure 3).

Thiamine triphosphate is a non-coenzyme derivative of thiamine, probably involved in acetylcholine neurotransmission (141–143). Unlike the substrate-specific enzyme that hydrolyzes ThTP, synthesis of ThTP is currently known to be catalyzed only by the enzymes producing ATP through ADP phosphorylation. That is, ThTP is synthesized by adenylate kinase 1 (AK1) in cytoplasm and by F₁F_o-ATP synthase in the mitochondria (140). Mitochondrial ThTP synthesis is tissue-specific and involves an unidentified regulator of F₁F_o-ATP synthase linked to pyruvate oxidation (140). Other isoenzymes of AK, shown in Figure 3, are expressed in the gut, having different cellular locations. Their ability to synthesize ThTP and the substrate specificity has not been tested.

The adenylated form of ThTP (AThTP) is found in mammalian tissues, but the enzyme(s) of its synthesis lose their activity during purification, and this interferes with their identification (140). Levels of ThTP and AThTP are higher in fast growing, non-differentiated cells, highlighting the significance of these thiamine derivatives in cellular differentiation (144).

Metabolic transformations and known roles of thiamine and its different natural derivatives are summarized in Table 2.

Table 2

Table 2. Metabolism and roles of thiamine and its derivatives in gastrointestinal system.

5 Transcriptomics of the enterocytes before and after gastric bypass implies increased thiamine demand in humans long term after the bypass

Thiamine deficiency often develops after bariatric surgery, including gastric bypass (145–147). Independent published data provide transcriptomics analysis of the enterocytes at one (15–45 days) (148) and 6–9 (115) months after gastric bypass. We have used these data to answer the question of what happens to thiamine-dependent metabolism after gastric bypass. To compare the levels of mRNAs for proteins involved in thiamine-associated metabolism across different experiments, they are normalized to the summarized transcripts of GAPDH+ACTB+TUBA1A, as described previously (149). The normalized levels are shown as averages between the datasets before and after gastric bypass (Figure 4), and in each of the analyzed datasets (Figure 5). As seen from Figures 4, 5, no gross changes are observed in human jejunum enterocytes 1 month after gastric bypass, similar to findings in the mouse model of the bypass, where an initial metabolic perturbation in response to the surgery, manifesting on the 9^th day, is reversed to the control state 2 months post-bypass (148). However, a comparison of either the average transcript levels (Figure 4A), or separate datasets of human enterocytes (Figure 5A) reveals minor, but consistent decreases in transcripts for ALPI > SLC19A1 ≈ OGDH ≈ HACL1 > SLC22A5 1 month after the bypass, suggesting a decrease in thiamine-dependent metabolism.

Figure 4

Figure 4. Changes in the averaged levels of mRNA for proteins of thiamine-dependent metabolism after gastric bypass. The relative abundance of mRNAs for the ThDP-dependent enzymes, thiamine transporters and enzymes of thiamine metabolism in jejunum enterocytes before and after gastric bypass is shown. The normalized mRNA signals are calculated as described in the legend to Figure 1. The data are shown as mean ± SEM. (A) Experiment GSE113819 {Platform GPL17586 (HTA-2_0) Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version], 5 datasets for enterocytes before bypass: GSM3120595, GSM3120597, GSM3120599, GSM3120601, GSM3120603 - and 5 datasets for enterocytes 1 month after bypass: GSM3120594, GSM3120596, GSM3120598, GSM3120600, GSM3120602}. (B) Experiment GSE214758 [Platform GPL20795, HiSeq X Ten (Homo sapiens), 9 datasets for enterocytes before bypass: GSM6615629, GSM6615631, GSM6615633, GSM6615637, GSM6615641, GSM6615643, GSM6615645, GSM6615647, GSM6615649 - and 9 datasets for enterocytes 6–9 months after bypass: GSM6615630, GSM6615632, GSM6615638, GSM6615640, GSM6615642, GSM6615644, GSM6615646, GSM6615648, GSM6615650].

Figure 5

Figure 5. Heatmaps showing transcriptomics changes in the thiamine-dependent metabolism after gastric bypass. The transcriptomics experiments are identified in Figure 4. The normalized mRNA signals for the enzymes of thiamine-dependent metabolism in jejunum enterocytes before and after gastric bypass are shown in decimal logarithm scale according to the color code legend accompanying each heatmap. The heatmaps are produced using R program. (A) The normalized mRNA signals before and 1 month after gastric bypass. (B) The normalized mRNA signals before and 6–9 months after gastric bypass. The dataset GSM6615633 is excluded, as many transcripts are not identified in this experiment.

Clusterization of transcript levels groups the transcripts according to their expression level and coincidence of the variations in different datasets (Figure 5). For instance, cluster 1a in Figure 5A comprises proteins whose transcript levels are higher than those of proteins in cluster 1b. Interestingly, each of the clusters comprises one of the well-characterized thiamine transporters: SLC19A3 shares the cluster 1a with thiamine/ThDP-dependent proteins of higher expression, while SLC19A2 shares the cluster 1b with thiamine/ThDP-dependent proteins of lower expression. Overall, the three well-defined clusters 1a, 2c, 2d comprise transcripts of the high, low and intermediary levels of expression, correspondingly. Transcripts of the thiamine/ThDP-dependent proteins which are most obviously decreasing 1 month after the bypass (Figure 4A, gene names in bold in Figure 5A) belong to the proteins with the high (ALPI, OGDH, HACL1, SLC22A5 in cluster 1a) or intermediary (SLC9A1in cluster 2d) expression (Figure 5A). Increasing levels of clusterization (2, 3 etc.) group proteins of increasing transcriptional coincidence, i.e., proteins whose transcript levels show coupled variations across the datasets. For instance, despite the overall similarity between the transcription profiles before and 1 month after the bypass, the transcripts show featured variations in each of the cluster 2a or 2b. In particular, variations in the transcripts for the ThDP-dependent enzymes with relatively high enterocytic expression, i.e., OGDH, HACL1 and PDHA1, are coupled with those in the transcripts for ALPI and ThDP transporter SLC44A4, all belonging to the cluster 2b including the three proteins with decreased transcripts 1 month after the bypass (Figure 5A in bold). The cluster 2a includes proteins with a lower, compared to the cluster 2b, expression, with the decreasing transcriptional level of SLC22A5 1 month after the bypass coupled to the variations of BCKDHB and SLC19A3 transcripts (Figure 5A). Reduced folate/ThDP exchanger SLC19A1, demonstrating decreased transcript levels 1 month after the bypass, shares the cluster (3 g) with mitochondrial ThDP transporter SLC25A19, ThTPase and BCKDHA (Figure 5A).

In contrast to the transcriptomic pattern 1 month after the bypass, human jejunum enterocytes 6–9 months after the bypass exhibit significant perturbations in their transcriptomics profiles (115). In particular, significant changes in the levels of transcripts of genes encoding TCA cycle enzymes and associated proteins are observed (115). In good agreement with this finding, our analysis of the selected transcripts characterizing thiamine-dependent metabolism, shows an overall increase in the transcripts for the related proteins (Figures 4, 5). In particular, up-regulation of the transcripts for the rate-limiting enzyme of the TCA cycle, 2-oxoglutarate dehydrogenase (OGDH), and for the TCA-cycle-affiliated proteins, such as subunits of pyruvate dehydrogenase (PDH) and branched chain 2-oxo acids dehydrogenase (BCODH) is observed. The upregulation of ThDP-dependent DHTKD1 protein 6–9 months after the gastric bypass surgery (Figures 4B, 5B) is similar to the long-term increase of DHTKD1 protein observed after another type of surgical intervention (laminectomy) (48). In accord with the upregulated expression of all the ThDP-dependent dehydrogenases, transcripts of the thiamine/ThDP transporters and the major producer of ThDP from thiamine, i.e., thiamine diphosphokinase (TPK), as well as probable ThMP phosphatase ACP1, also undergo a long-term upregulation (Figure 4B).

Heatmap in Figure 5B shows that the first level of clusterization results in separating the ThDP-dependent TKT in cluster 1a from all the other ThDP-dependent enzymes, combined in cluster 1b. That said, the homooligomeric ThDP-dependent enzymes participating in metabolism of glucose and amino acids, i.e., TKT, OGDH, DHTKD1, preserve their cluster partners in both of the studied experiments. TKT has a common cluster (2d in Figures 1A, 5A in Figure 5B) with ThTPA and SLC19A1; OGDH shares its cluster (1a in Figures 5A,C in Figure 5B) with SLC44A4 and SLC19A3; DHTKD1 keeps associated with SLC19A2 and SLC22A4 (clusters 3 h in Figures 5A,D in Figure 5B). For the heterooligomeric ThDP-dependent enzymes, i.e., PDH and BCOADH, the cluster partners of the subunits α and β may be switched. For instance, OGDH shares the cluster 3d with PDHA1 in Figure 5A, while in Figure 5B OGDH occupies the same cluster 5c with PDHB, while PDHA1 is moved to the DHTKD1-comprising cluster 5d. BCKDHB partners with ALPI in the cluster 1a in Figure 5A, but the ALPI partner in cluster 4e of Figure 5B is BCKDHA. As a result, a comparison of the two independent transcriptomics experiments reveals stable associations between specific proteins of thiamine/ThDP metabolism and ThDP-dependent enzymes in enterocytes.

The observed upregulation of not only the ThDP-dependent dehydrogenases limiting and feeding the TCA cycle, but also the thiamine transporters and ThDP-producing enzyme TPK1 (Figures 4B, 5B), may compensate for decreased levels and/or increased demands of thiamine in enterocytes 6–9 months after gastric bypass, facilitated by the higher expression of the proteins binding thiamine or ThDP. Indeed, increased activities of ThDP-dependent dehydrogenases (assayed in vitro) are known as a compensatory response to their in vivo inhibition (38, 150). Cellular exposure to TD strongly stimulates OGDH activity upon the following incubation with ThDP (29). Hence, the upregulation of the thiamine/ThDP-dependent protein transcripts 6–9 months after gastric bypass (Figures 4B, 5B) maybe a biochemical indicator of thiamine insufficiency in the enterocytes. Remarkably, the upregulation is observed after initial decrease in the five thiamine/ThDP-dependent proteins 1 month after the bypass (Figures 4A, 5A).

According to the overall transcriptomics analysis of the long-term (6–9 months) changes in jejunum enterocytes after the bypass (115), there are interactions between the TCA cycle gene cluster and other significantly affected pathways that include genes linked to the cell cycle G2/M DNA damage checkpoint regulation. The DNA damage checkpoint is also involved in cellular repair and differentiation (151). Strong involvement of thiamine transporters in the differentiation of enterocytes is suggested by approximately 6-fold higher thiamine uptake, corresponding to elevated expression of SLC19A2 and SLC19A3, in the differentiated jejunum epithelial cells of the villi, compared to the non-differentiated jejunum epithelial cells of the crypt (152). Positive action of high doses of thiamine under metabolic perturbations including surgeries (48, 115, 149, 153–155) suggest that cellular protection and/or repair requiring cell differentiation, increase demand of thiamine. After surgical perturbations in enterocytic integrity, elevated cellular growth and differentiation, which occur within days, is followed by temporal organ-specific adaptations to the bypass (148). Both phases are characterized by high metabolic demands. If the increased demand is not met by the increased thiamine supply, a state of TD may easily follow, first in enterocytes, and after some period at the level of organism, most often manifested as Wernicke encephalopathy (145–147). Thus, bariatric surgery-induced upregulation of thiamine-dependent metabolism in jejunum (Figures 4B, 5B) may manifest metabolic remodeling addressing insufficient levels of thiamine in post-bypass enterocytes.

6 Molecular basis of the thiamine-induced improvements in gastrointestinal disorders

As mentioned above, despite the direct exposure of the gastrointestinal system to nutritional supply, this system often exhibits thiamine-responsive dysfunctions even when no known TD signs are evident in other tissues. The essential role of ThDP in mitochondrial energy production (Figure 1) is universal for all tissues and therefore can hardly explain specific and primary vulnerability of the gastrointestinal system. This energetic role is usually considered in the context of the specific vulnerability to TD of tissues with high energy demand, such as the heart and the brain. These tissues are considered to be the last to decrease their thiamine content during TD and the first to replete it upon thiamine administration, with the liver serving as the thiamine depot for other tissues (156). Nevertheless, if drugs inhibit ThDP-dependent enzymes, as, e.g., omeprazole does (157), direct exposure of the gastrointestinal system to oral drugs may increase its vulnerability compared to other tissues. Based on currently available data, we also suggest several other origins of TD restricted to the gastrointestinal system.

First of all, the dual role of the gastrointestinal system - dedicated not only to nutrient absorption from the lumen, but also to their supply to other tissues via the blood - may present one of the reasons for the intestine-specific susceptibility to TD. That is, the function of the gastrointestinal system requires a balance between thiamine absorption and its supply to other tissues. Mechanisms controlling the balance between the two processes are not well characterized. However, as shown above in the analysis of thiamine/ThDP-dependent metabolism in enterocytes after gastric bypass, increased thiamine demand by other tissues may impair the thiamine status of the gastrointestinal system.

Furthermore, a major difference between the gastrointestinal system and other tissues is the gut microbiome. It is important to note that microbes can synthesize not only thiamine but also thiamine antagonists. This may occur as part of natural metabolism (158) or when microbes are exposed to drugs that undergo aberrant reactions, such as metronidazole (159–161). Microbial enterotoxins alter gene expression in the gut epithelium, leading to enteropathies (162). Proinflammatory cytokines inhibit enterocytic thiamine uptake at the transcriptional level (163). Thiamine transport is also significantly reduced by bacterial lipopolysaccharide and in sepsis (163, 164). Yet another mechanism contributing to the specific vulnerability of the gastrointestinal system to TD may involve the gut-brain axis, which depends on vagal tone and acetylcholine neurotransmission, for which thiamine co-release is known (165–167). In addition to the parasympathetic regulation, acetylcholine is currently suggested to be a paracrine signal of peripheral tissues, particularly in pancreas and epithelial cells (168, 169).

Below, data on molecular mechanisms underlying thiamine-responsive gastrointestinal dysfunctions are considered in more detail.

6.1 Exposure of the intestine to bacterial enzymes degrading thiamine or producing thiamine antagonists

Abnormal proliferation of gut bacteria in the upper small intestine, defined by the term ‘small intestinal bacterial overgrowth’ (SIBO), is associated with symptoms such as bloating, abdominal pain, excessive foul-smelling flatulence, constipation and/or diarrhea. The etiology of SIBO is varied and complex, with relapse commonly occurring after conventional treatment. Research suggests that SIBO is involved in up to 78% of irritable bowel syndrome (IBS) cases, the most commonly diagnosed disorder of gastrointestinal motility (170). SIBO has been associated with dysfunctional intestinal motility, characterized by inadequate peristaltic action of gastrointestinal smooth muscle (171–174), hypochlorhydria, and pancreatic exocrine insufficiency (175). As mentioned above, such symptoms are often responsive to thiamine administration. A link between SIBO and TD is provided by the notion that SIBO may increase bacterial transformations of thiamine, which are far more variable than those in mammals. In particular, this concerns the action of bacterial thiaminases I and II, which catalyze biosynthesis of thiamine antagonists or thiamine degradation, respectively. Thiaminase I catalyzes the substitution of thiamine heterocycles with catalytically inactive heterocycles from xenobiotics. For instance, in the presence of the antibiotic metronidazole, thiaminase I substitutes the thiazolium ring of thiamine with the imidazolium ring of metronidazole, resulting in a thiamine antagonist which can inhibit TPK (160). Microbes also synthesize naturally occurring thiamine antagonists, such as 2’-methoxyThDP (158). Thiaminase II degrades thiamine, splitting it into two heterocycles. Immobilized thiaminase II has been applied as an anticancer approach to deplete thiamine in cancer cells (176). Thiaminase II in fern extracts exerts an effect on neurotransmission which, similar to that of the thiamine antagonist pyrithiamine, can be counteracted by the addition of thiamine (177). It may thus be suggested that direct exposure of the gut to the microbiome - capable of synthesizing thiamine antagonists or possessing thiamine-degrading thiaminase II - may first of all affect the gut, leading to gastrointestinal beriberi, especially under conditions of SIBO or other disturbances of the gut microbiome. If these conditions are not treated, TD may spread from the gastrointestinal system to other tissues, as observed in metronidazole-induced encephalopathy (159).

6.2 Thiamine supports intestinal barrier integrity

Disturbed intestinal barrier function has been increasingly studied in recent years and is now widely recognized as a prominent feature of many chronic diseases, pertaining not only to the gut (178), but also to neurological, psychiatric, cardiovascular, and autoimmune conditions. Increased intestinal permeability can facilitate the entry of food antigens, bacteria and bacterial components into systemic circulation, which are thought to provoke systemic inflammatory responses. The intestinal epithelial barrier shares several key morphological and functional characteristics with the blood–brain barrier. The tight junctions of both structures are composed of transmembrane proteins such as claudin, occludin, zonula occluden (ZO), and endothelial cell-selective adhesion molecules. Thiamine is necessary for maintaining intestinal epithelial cell bioenergetics, and reduced activity of thiamine-dependent enzymes may lead to a defective gut barrier (34). TD is known to disrupt the blood brain barrier, featuring loss of occludin, ZO-1 and ZO-2 (179, 180). It is therefore possible that similar mechanisms could be at play in the gastrointestinal tract. Notably, TD leads to disturbed expression of junction protein subtypes in Ctenopharyngodon idella (181). Moreover, thiamine administration enhances claudin-1, claudin-4, ZO-1, and occludin in ruminal epithelium (22). Thiamine facilitates the protective action of secretory IgA against immunogenic threats in enterocytes (182). The prevention of intestinal barrier dysfunction by secretory IgA, along with its immunomodulatory properties, may play a role in IBD, including ulcerative colitis and Crohn’s disease (183). Poor thiamine status is frequently reported in IBD, mostly assumed to be a consequence of malabsorption (184–187). TD aggravates ulcerative colitis in mice, associated with the promoted infiltration of proinflammatory M1 macrophages into colonic lamina propria (188). The underlying mechanism is TD-induced impairment of PDHC activity, which causes remodeling of glucose metabolism in the macrophages.

Other mechanisms may also contribute to IBD, too. In particular, IBD is associated with hypoxia in enterocytes (189). Hypoxia has recently been shown to downregulate thiamine transporters in a colonic cell line, impairing the thiamine uptake that results in a localized intracellular deficiency (190). Alkaline phosphatase of the brush border of the intestines, which participates in thiamine transport (Figure 2), plays protective roles against pathogenic infection (191) and bacterial toxins (LPS) in the gut, and may counteract inflammation (192).

A combination of exogenous and endogenous factors is known to influence intestinal barrier function, including both acute and/or chronic immune dysregulation (193). TD may induce negative developments in the gastrointestinal system, which can be counteracted by thiamine supplementation. By normalizing or increasing the efficiency of glucose metabolism, thiamine decreases the degradation of amino acids as energy substrates and increases protein synthesis, which is essential for maintaining tight junctions and intestinal barrier integrity (194). Remarkably, TD in rats causes a 42–66% reduction of brush border enzyme activities and a 20% reduction in intestinal weight with significant thinning of the microvillus membrane (195). This may be the result of disturbed function of the ThDP-dependent 2-oxoglutarate dehydrogenase (Figure 1), as a universal effect of its inhibition is perturbation in relative amino acid abundance and a subsequent decrease in protein synthesis (196, 197). In turn, villous atrophy can impair nutrient absorption and is one of the primary mechanisms underpinning extensive nutritional deficiencies found in Celiac and inflammatory bowel diseases (198). Furthermore, atrophy and inflammation of mucosal surfaces in the gut are documented in experimental TD (82), and high doses of thiamine have been trialed in two studies showing that thiamine reduced fatigue associated with IBD (199, 200).

6.3 Thiamine and intestinal inflammation

Thiamine is considered a natural anti-inflammatory compound (201). Studies in both goats (202) and cows (203) indicate that thiamine has anti-inflammatory effects on the ruminal epithelium, ameliorating in particular the intestinal inflammation and barrier permeability caused by high-concentrate diet (27). As considered in Section 3 above, thiamine affects the composition of symbiotic microbiota, and ThDP-dependent bacterial butyrate synthesis in particular. Knockouts of the ThDP transporter SLC44A4 in mice display an upregulation of genes associated with colonic inflammation, and increased susceptibility to dextran sodium sulfate-induced colitis, accompanied by significant weight loss and shortening of the colon (204).

A number of positive anti-inflammatory actions of thiamine include activation of Nrf2 and decreased ROS levels, enhanced activity of mitochondrial respiratory chain complexes I-IV, downregulation of endoplasmic reticulum stress, and suppression of gene expression associated with mitophagy, oxidative stress, and proinflammatory cytokines (205). Remarkably, the antioxidant effects of thiamine, including Nrf2 activation, are observed even when the thiamine disulfide forms— which should decrease cellular redox potential— are administered (206). These pharmacological forms, such as sulbutiamine or TTFD, may penetrate cell membrane better than thiamine. Inside the cells, they are reduced, particularly by the thioredoxin and glutaredoxin system, simultaneously stimulating antioxidant defense through Nrf2 activation (206). One may suggest that Nrf2 activation is specific to the disulfide forms of thiamine, not inherent in thiamine itself, as only the thiamine disulfides undergo intracellular reduction. The associated shift in the cellular redox state may cause the activation of Nrf2, leading to this additional positive effect on metabolism of high doses of thiamine disulfides, compared to thiamine. The Nrf2 activation along with the thiamine formation may explain why high doses of the thiamine disulfides, expected to decrease cellular redox potential, do not have any negative action even upon the long-term administration (155).

By maintaining integrity of the intestinal barrier via complex mechanisms, the vagus nerve and its major neurotransmitter, acetylcholine (ACh), play a crucial role in coordinating adaptive neural and endocrine responses of the gastrointestinal system, including those against infection and inflammation (207). SIBO and intestinal hypomotility are highly prevalent in patients with anti-ACh receptor antibodies (208, 209).

6.4 Acetylcholine-dependent mechanisms of thiamine action in gastrointestinal disorders

Many independent data associate gastrointestinal disorders with perturbed ACh signaling. ACh exerts tonic effects to maintain constriction of the lower esophageal sphincter, with ACh signaling compromised in a rat model of gastrointestinal reflux disease (210). Drugs which block the action of ACh can cause abnormal relaxation of the sphincter and reflux (211). The anti-cholinergic agents atropine and papaverine induce constipation in animals (23). In contrast, preserving ACh levels through acetylcholinesterase inhibitors improves gastrointestinal reflux disease (212). Pro-cholinergic pharmacological agents also provide symptomatic improvement in FGIDs (213, 214). Pharmacological forms of thiamine exhibit interactions with the ACh-dependent effects. That is, TTFD prevents the gut paralysis induced by atropine or papaverine (23), while sulbutiamine promotes cholinergic neurotransmission, potentiating the action of ACh esterase inhibitors (215). Interestingly, structure of an inhibitor of ACh esterase, acotiamide (brand name acofide), approved in Japan as a prokinetic motility drug against functional dyspepsia (216–218), combines the two heterocycles, one of them thiazole, that may be considered as structural mimics of the thiazolium and aminopyrimidine heterocycles of thiamine.

An intimate relationship between thiamine and cholinergic neurotransmission relies on both the coenzyme and non-coenzyme actions of thiamine and its derivatives. In cholinergic cells, the ThDP-dependent pyruvate dehydrogenase complex synthesizes the ACh precursor acetyl-CoA (Figure 1) (219, 220). Distribution of this acetyl-CoA between the oxidation in the TCA cycle and participation in ACh synthesis is regulated through limitation of the TCA cycle rate by the ThDP-dependent OGDH, and the non-coenzyme action of thiamine and derivatives on the other enzymes involved (221). TD-perturbed function of ThDP-dependent dehydrogenases of TCA cycle (Figure 1) is a well-known contributor to impaired synthesis of ACh (219). The ensuing mitochondrial dysfunction of cholinergic cells increases their susceptibility to different insults (222–224). In particular, neurons of the gastrointestinal system are highly sensitive to oxidative stress (219, 225, 226), which is a general hallmark of TD (227). Cytosolic oxidative stress can inactivate nicotinic ACh receptors in neurons, decreasing ACh-evoked currents (228). Oxidative stress is considered to contribute to the pathophysiology of IBD (229, 230).

Independent of metabolic action as the coenzyme ThDP, thiamine is essential for axonal membrane excitability, playing a significant role in development of the action potential (231). This non-coenzyme action of thiamine in neuronal signaling is further supported by the identified molecular targets of thiamine and its non-coenzyme derivative ThTP. ThTP-dependent phosphorylation of rapsyn regulates ACh neurotransmission, as rapsyn is a scaffolding protein of the post-synaptic membrane of neuromuscular junctions, specifically associated with nicotinic ACh receptors (232). Hydrolysis of ThTP by synaptic membrane-bound protein(s) different from the well-characterized soluble ThTPase of cytosol, is supposed to be involved with synaptic function (233, 234). Thiamine binds to a bitter taste receptor, which modifies ileum contraction (235) and provokes ACh-induced contraction of jejunum (236). At a high concentration (0.05 mM), thiamine also binds to an isolated nicotinic ACh receptor (237). Probably, the low binding affinity is an artifact of the receptor isolation, as the physiologically relevant affinity of thiamine to the receptor may require protein–protein interaction within the native structure of the synapses. The non-coenzyme action of thiamine in neurotransmission is further supported by the action of the thiamine analog oxythiamine, whose diphosphorylated derivative (oxyThDP), formed by TPK in vivo, is an antagonist of the coenzyme action of thiamine. As a result, oxyThDP inhibits ThDP-dependent dehydrogenases, whose function is required for ACh synthesis, particularly pyruvate dehydrogenase complex generating ACh precursor acetyl-CoA (Figure 1). However, in superfused rat brain slices, oxythiamin enhances the release of synaptic acetylcholine, thus mimicking the non-coenzyme action of thiamine in facilitating ACh neurotransmission (238).

ACh is a major neurotransmitter of vagus nerve, coordinating the brain-gut axes (239). Vagus nerve stimulation can lead to gastrointestinal improvements (240), e.g., can increase gastric emptying through acting on the pyloric sphincter (241) and may be a treatment for gastroparesis (242). Potentiation of ACh release through transcutaneous vagal neuromodulation is known to enhance gut motility, reduce inflammation by suppressing TNF-α, and preserve epithelial tight junction integrity through the activation of enteric glial cells (243). The data indicate that TD-perturbed ACh synthesis and neurotransmission in vagus nerve may induce gastrointestinal dysfunction. However, in this case ACh synthesis and signaling are controlled by neurons of central nervous system. As discussed above, sufficient levels of thiamine in the brain are supported at the expense of other tissues, causing TD in these other tissues long before the TD occurs in the brain. Hence, when TD is limited to the gastrointestinal system, the pathology-relevant perturbation of ACh signaling may rather be expected in the enteric nervous system (ENS). ENS may regulate gastrointestinal function both with and without the input of central nervous system (244–246). In particular, the independent function of ENS is manifested in the intestinal peristaltic reflex.

ACh is a primary neurotransmitter of different types of ENS neurons, i.e., intrinsic primary afferent neurons, excitatory motor neurons, and interneurons (245). The thiamine-induced increases in contractions of the intestine smooth muscle and peristalsis, observed in a number of independent in vitro experiments on muscle strips of gastrointestinal tract (207, 219–222), support the action of thiamine on ACh signaling in ENS in the absence of central regulation (236). Synaptic co-release of thiamine and ACh, with thiamine facilitating the neurotransmission through its non-coenzyme action, is known from the early works of Minz and von Muralt on neuromuscular junctions (143, 165–167). In accordance with these observations, addition of thiamine and ThMP modulate synaptic transmission, electric and contractile activity of the smooth muscle strips from gastrointestinal tract (247). Thiamine hydrochloride, thiamine nitrate, thiamine propyldisulfide, TTFD, and some other experimental derivatives increase peristalsis of isolated sections of small intestine from rat (248). In isolated sections of murine jejunum and ileum, thiamine regulates parameters of the ACh-induced contraction in the concentration-dependent manner (236). In isolated duodenal and jejunal segments from cats, dogs, rabbits and guinea pigs the thiamine disulfide derivative TTFD exerts an excitatory effect on motility; sensitivity of the effect to an antagonist of muscarinic acetylcholine receptor atropine suggests involvement of the ACh neurotransmission (249). All forms of thiamine including thiamine hydrochloride, S-benzoyl thiamine disulfide, TTFD, increase contractions of isolated segments of small intestine (248).

In human patients with chronic enterocolitis and colitis, thiamine-induced increases in motor activity of stomach, small and large intestine are detected by electrogastrography and balloon-kymography, with no similar effects of vitamins B6, B12 and C (250). In animal models, TTFD can stimulate intestinal peristalsis within a few minutes after administration, also when mesenteric nerves are cut (251, 252). Intravenous administration of TTFD induces a slight increase in tone and a remarkable increase in the amplitude of rhythmic contractions in the jejunal loop of both non-anesthetized and anesthetized dogs for 20 min (253). TTFD applied topically inside the lumen of the intestine can also elicit such excitation effect (249). Nevertheless, in some studies on TTFD, percent weight gain was lower in the TTFD group (254). Two possible explanations for this effect are proposed: (1) reduced food intake due to an irritant effect of the treatment on the gastrointestinal tract (255, 256) or (2) a stimulant effect of TTFD on metabolism via enhanced noradrenaline secretion and thermogenesis (257).

ACh signaling in non-neuronal cells, employing the nicotinic and muscarinic ACh receptors, is comparable to ACh neurotransmission (168, 169). Therefore, ACh signaling in non-neuronal cells is another process potentially affected by TD before the deficiency develops into the well-known dry or wet beriberi. Glial cells of ENS are stimulated by ACh (245). In mammalian intestine, ACh regulates sodium currents in the apical and basolateral membranes (168). In human pancreas, ACh synthesized by alpha-cells is suggested to activate beta-cells (169). Other in vivo actions of ACh in pancreas may be linked to its high thiamine content (89). As considered in Section 3, TD in pancreas strongly affects gastrointestinal function. Perturbed ACh signaling by pancreatic cells under TD may contribute to gastrointestinal dysfunction in addition to insufficient pancreatic synthesis of the enzymes needed for digestion.

Thus, cholinergic neurotransmission and ACh-dependent regulation of non-neuronal cells employ ThDP coenzyme function for ACh synthesis and non-coenzyme action of thiamine in ACh signaling. Both may be impaired by TD, contributing to gastrointestinal dysfunction.

7 Conclusion

Various lines of evidence at the molecular level and in model systems provide a mechanistic basis for a number of observations at the physiological level, where thiamine supplementation alleviates gastrointestinal dysfunction, suggesting that insufficient levels of thiamine may underlie this dysfunction. The specific vulnerability of the gastrointestinal system to TD, in the absence of more common signs of wet and dry beriberi, may arise from direct exposure of this system to the gut microbiome and oral drugs, along with the continuous need to redistribute thiamine to other tissues. Cholinergic neurons of the enteric nervous system, which interact closely with gastrointestinal epithelial cells, as well as ACh signaling in non-neuronal cells such as enteric glia and pancreatic cells, depend on the essential role of thiamine in ACh production and the facilitation of ACh signaling. These mechanisms collectively contribute to the unique vulnerability of the gastrointestinal system to TD, even in the absence of classic forms of TD such as dry and wet beriberi. The multitude of proteins involved in mammalian metabolism and function of thiamine is not fully characterized. However, available data point to a much greater complexity of thiamine metabolism and its physiological significance, as commonly considered. This complexity must still be addressed by the identification of all genes involved. Individual differences in genetic variants of the thiamine-dependent proteins, in combination with environmental factors, may underlie personal vulnerabilities to thiamine deficiency, manifesting in an ever-increasing variety of clinical presentations.

Author contributions

EO: Investigation, Software, Visualization, Writing – original draft, Writing – review & editing. AE: Data curation, Formal analysis, Investigation, Methodology, Software, Visualization, Writing – original draft, Writing – review & editing. VB: Conceptualization, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Visualization, Writing – original draft, Writing – review & editing.

Funding

The author(s) declare that financial support was received for the research and/or publication of this article. The work of VB was conducted under the state assignment of Lomonosov Moscow State University.

Acknowledgments

A language engine was used to check grammar and phrasing. The authors gratefully acknowledge the support of Objective Nutrition Ltd., 29 Gildredge Road, Eastbourne, East Sussex, United Kingdom, in providing APC for this manuscript.

Conflict of interest

EO is an owner of a nutraceutical company that sells vitamin supplements including thiamine.

The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.

Generative AI statement

The authors declare that no Gen AI was used in the creation of this manuscript.

Publisher’s note

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