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ORIGINAL RESEARCH article

Front. Nutr.
Sec. Nutrition and Food Science Technology
Volume 12 - 2025 | doi: 10.3389/fnut.2025.1514179
This article is part of the Research Topic Nanotechnology in the Food Sector: Advances and Challenges View all 4 articles

Ameliorative Effect of Liprotide-Encapsulated Vitamin D3 on Blood Glucose, Calcium Homeostasis, and Vitamin D level in a Vitamin D and Calcium Deficient Rat Model

Provisionally accepted
Gemala Anjani Gemala Anjani 1*Reza Achmad Maulana Reza Achmad Maulana 2Sylvia Rahmi Putri Sylvia Rahmi Putri 1Faizah Fulyani Faizah Fulyani 3Ahmad Syauqy Ahmad Syauqy 1Diana Nur Afifah Diana Nur Afifah 1Fitriyono Ayustaningwarno Fitriyono Ayustaningwarno 1Refani Alycia Kusuma Refani Alycia Kusuma 1Zulfatul Masruroh Zulfatul Masruroh 1
  • 1 Department of Nutrition Science, Faculty of Medicine, Diponegoro University, Semarang, Indonesia
  • 2 Ahmad Dahlan University, Yogyakarta, Special Region of Yogyakarta, Indonesia
  • 3 Faculty of Medicine, Diponegoro University, Semarang, Central Java, Indonesia

The final, formatted version of the article will be published soon.

    Vitamin D3, recognized for its higher bioavailability and direct cell utilization, plays a vital role in the human body. Applying β-lactoglobulin with oleic acid (β-Lg-AO) as an encapsulating agent is anticipated to protect and enhance the transport of vitamin D3 to the gastrointestinal system. This study aimed to evaluate effect of liprotide-encapsulated vitamin D3 in a vitamin D-deficient rat on blood glucose, Vitamin D and calcium status. This is pre-post intervention. 24 mice were divided into 4 groups: (K-) normal rats; (K+) rat model of vitamin D and calcium deficiency; (P1) rat model of vitamin D and calcium deficiency with vitamin D3 intervention; (P2) rat model of vitamin D and calcium deficiency with liprotide-encapsulated vitamin D3 intervention. The administered dose of vitamin D3 was 180 IU (2 ml solution). Liprotide-encapsulated vitamin D3 intervention in vitamin D and calcium deficiency rats can significantly increase vitamin D (25(OH)D) and calcium levels (p<0.05). The increase in vitamin D (25(OH)D) level was 53.69 ng/ml, and the increased calcium level was 4.38 mg/dl. Blood glucose levels of vitamin D-calcium deficiency rats decreased significantly (p<0.05) by 39.87 mg/dl. Vitamin D3 encapsulated liprotide improves vitamin D and calcium in the blood more effectively than vitamin D3 without encapsulation in deficient rats.

    Keywords: encapsulation, Vitamin D, Blood Glucose, Calcium, Liprotide, Vitamin D3

    Received: 20 Oct 2024; Accepted: 07 Jan 2025.

    Copyright: © 2025 Anjani, Achmad Maulana, Putri, Fulyani, Syauqy, Afifah, Ayustaningwarno, Kusuma and Masruroh. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Gemala Anjani, Department of Nutrition Science, Faculty of Medicine, Diponegoro University, Semarang, Indonesia

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.