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ORIGINAL RESEARCH article

Front. Nutr.
Sec. Nutrition and Metabolism
Volume 11 - 2024 | doi: 10.3389/fnut.2024.1511682

Sesamin alleviates lipid accumulation induced by elaidic acid in L02 cells through TFEB regulated autophagy

Provisionally accepted
Xueli Liang Xueli Liang Tianliang Zhang Tianliang Zhang Xinyi Cheng Xinyi Cheng Hang Yuan Hang Yuan Ning Yang Ning Yang Yanlei Yi Yanlei Yi Xiaozhou Li Xiaozhou Li Fengxiang Zhang Fengxiang Zhang Jin-Yue Sun Jin-Yue Sun Zhenfeng Li Zhenfeng Li Xia Wang Xia Wang *
  • Shandong Second Medical University, Weifang, Shandong Province, China

The final, formatted version of the article will be published soon.

    Non-alcoholic fatty liver disease (NAFLD) is a common chronic disease seriously threatening human health, with limited treatment means, however. Sesamin, a common lignan in sesame seed oil, exhibi ts anti-inflammatory, antioxidant, and anticancer properties. Our previous studies have shown an ame liorative effect of sesamin on lipid accumulation in human hepatocellular carcinoma (HePG2) induce d by oleic acid, with its protective effects unclear in the case of 9-trans-C18:1 elaidic acid (9-trans-C1 8:1). L02 cells, an important tool in scientific researches due to its high proliferation ability, preserve d hepatocyte function, and specificity in response to exogenous factors, were incubated with 9-trans-C18:1 to establish an in vitro model of NAFLD in our study. The lipid accumulation in cells and the morphology of mitochondria and autolysosomes were observed by Oil Red O staining and transmissi on electron microscopy. The effects of sesamin on oxidative stress, apoptosis, mitochondrial function, autophagy as well as related protein levels in L02 cells were also investigated in the presence of 9-tra ns-C18:1. The results showed that sesamin significantly accelerated the autophagy flux of L02 cells i nduced by 9-trans-C18:1 as well as elevated protein levels of transcription factor EB (TFEB) and its d ownstream target lysosome-associated membrane protein 1(LAMP1), along with up-regulated levels of TFEB and LAMP1 in the nucleus indicated by Immunofluorescence. In addition, PTEN-induced p utative kinase 1 and Parkin mediated mitophagy was activated by sesamin. The direct inhibitor Eltro mbopag and indirect inhibitor MHY1485 of TFEB reversed the protective effect of sesamin, suggesti ng the involvement of autophagy in the lipid-lowering process of sesamin. This work suggests that se samin regulates autophagy through TFEB to alleviate lipid accumulation in L02 cells induced by 9-tr ans-C18:1, providing a potential target for the prevention and treatment of NAFLD.

    Keywords: NAFLD, elaidic acid, sesamin, autophagy, mitophagy. Abbreviations list 9-trans-C18:1, 9-trans-C18:1 elaidic acid, NAFLD, non-alcoholic fatty liver disease, TEM, transmission electron microscopy, PINK1, PTEN-induced putative kinase 1, LAMP1, lysosomeassociated membrane protein 1, EO, Eltrombopag, mTOR, mechanistic target of rapamycin kinase, mTORC1, mechanistic target of rapamycin kinase complex 1

    Received: 15 Oct 2024; Accepted: 11 Dec 2024.

    Copyright: © 2024 Liang, Zhang, Cheng, Yuan, Yang, Yi, Li, Zhang, Sun, Li and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Xia Wang, Shandong Second Medical University, Weifang, 261053, Shandong Province, China

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