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ORIGINAL RESEARCH article

Front. Nutr.
Sec. Nutritional Immunology
Volume 11 - 2024 | doi: 10.3389/fnut.2024.1510028
This article is part of the Research Topic Marine Peptides: Unveiling Their Role in Bone Immunomodulation and Joint Health View all 3 articles

Evaluation of nutrient composition and bone-promoting activity of miiuy croaker (Miichthys miiuy) bone

Provisionally accepted
Qianqian Ouyang Qianqian Ouyang 1Lifen Liu Lifen Liu 1Lili Liu Lili Liu 2*Yi Li Yi Li 1Yi Qi Yi Qi 1*Kefeng Wu Kefeng Wu 1*Guoping Zhu Guoping Zhu 3*Hua Ye Hua Ye 1*
  • 1 Guangdong Medical University, Zhanjiang, China
  • 2 Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong Province, China
  • 3 Guangdong Ocean University, Zhanjiang, Guangdong Province, China

The final, formatted version of the article will be published soon.

    To promote the high-value utilization of the processing byproducts of farmed miiuy croaker (Miichthys miiuy), we evaluated the nutrient composition of fish bone from farmed M. miiuy, prepared M. miiuy bone peptide (MMBP), and evaluated its osteogenic activity. The nutritional content of M. miiuy bone was assessed, including an analysis of its fundamental nutrients, amino acids, total sugars, and minerals. Furthermore, the osteogenic potential of MMBP was investigated using molecular docking, an MC3T3-E1 cell proliferation assay, and zebrafish growth enhancement models. The effects of MMBP on osteoporosis (OP) were assessed using a rat model of retinoic acid-induced OP. The results revealed that the cultured bones of M. miiuy consist of 11.40 % moisture, 59.30 % ash, 1.60 % crude fat, 27.10 % crude protein, and 0.58 % total sugars. In terms of its amino acid profile, total amino acids made up 22.13 %, with essential amino acids comprising 4.33 %, nonessential amino acids comprising 17.80 %, and free amino acids accounting for 11.83 %. M. miiuy bone exhibited substantial mineral content, with calcium, phosphonium, and selenium levels measured at 162.511 mg/kg, 7.151 mg/kg, and 0.264 mg/kg, respectively. MMBP significantly enhanced the proliferation of MC3T3-E1 cells in a dose-dependent manner. Furthermore, in a retinoic acid-induced OP rat model, treatment with MMBP effectively increased serum calcium and phosphorus levels, alleviated calcium loss, and reduced tartrate-resistant acid phosphate and alkaline phosphatase activities. MMBP treatment significantly improved bone mineral density. Given its osteogenic properties, MMBP holds promise as a bioactive component in functional foods, potentially contributing to bone growth and overall skeletal health.

    Keywords: Miichthys miiuy bone, Nutrient composition, peptide, bonepromoting activity, Osteoporosis

    Received: 12 Oct 2024; Accepted: 09 Dec 2024.

    Copyright: © 2024 Ouyang, Liu, Liu, Li, Qi, Wu, Zhu and Ye. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Lili Liu, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong Province, China
    Yi Qi, Guangdong Medical University, Zhanjiang, China
    Kefeng Wu, Guangdong Medical University, Zhanjiang, China
    Guoping Zhu, Guangdong Ocean University, Zhanjiang, 130012, Guangdong Province, China
    Hua Ye, Guangdong Medical University, Zhanjiang, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.