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ORIGINAL RESEARCH article

Front. Nutr.
Sec. Nutritional Epidemiology
Volume 11 - 2024 | doi: 10.3389/fnut.2024.1473196

Inverted U-Shaped Relationship between Serum Vitamin B12 and α-Klotho Levels in US Adults: A Cross-Sectional Study

Provisionally accepted
Yushan Li Yushan Li Xingji Gong Xingji Gong *Wenjie Du Wenjie Du *Yang Li Yang Li *Dongyong He Dongyong He *Jian Yao Jian Yao *Cui Bai Cui Bai *
  • The Affiliated Hospital of Qingdao University, Qingdao, China

The final, formatted version of the article will be published soon.

    Background: Serum vitamin B12 and α-Klotho are important markers associated with aging. Limited studies have been conducted on the relationship between vitamin B12 and α-Klotho. Objectives: This study investigated the relationship between circulating α-Klotho and vitamin B12. Methods: A total of 4502 American adults with circulating vitamin B12 levels and α-Klotho levels from the National Health and Nutrition Examination Survey (2011-2014) were included. A weighted multiple linear regression model was used to evaluate the correlation between vitamin B12 and α-Klotho levels. To clarify potential nonlinearities, smoothed curve fitting and threshold effects analysis were employed. Results: A statistically significant nonlinear relationship was found between vitamin B12 levels and circulating α-Klotho levels after adjusting for potential confounders. We observed an inverted U-shaped relationship between serum vitamin B12 levels and circulating α-Klotho levels. Notably, serum vitamin B12 levels below the threshold (1020 pg/mL) exhibited a positive correlation with circulating α-Klotho levels (β = 0.14, 95% confidence interval (CI): 0.09-0.18, P < 0.0001). Conversely, serum vitamin B12 levels above the threshold (1020 pg/mL) exhibited a negative correlation with circulating α-Klotho levels (β = -0.12,95% CI: -0.17--0.06, P < 0.0001). Sensitivity analyses were performed and consistent results were obtained. Conclusions: This study demonstrated an inverted U-shaped relationship between circulating vitamin B12 and α-Klotho in American adults. The optimal concentration of serum vitamin B12 in American adults was found.

    Keywords: Vitamin B12, cobalamin, α-klotho, Homocysteine, Oxidative Stress, DNA Methylation

    Received: 30 Jul 2024; Accepted: 14 Oct 2024.

    Copyright: © 2024 Li, Gong, Du, Li, He, Yao and Bai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Xingji Gong, The Affiliated Hospital of Qingdao University, Qingdao, China
    Wenjie Du, The Affiliated Hospital of Qingdao University, Qingdao, China
    Yang Li, The Affiliated Hospital of Qingdao University, Qingdao, China
    Dongyong He, The Affiliated Hospital of Qingdao University, Qingdao, China
    Jian Yao, The Affiliated Hospital of Qingdao University, Qingdao, China
    Cui Bai, The Affiliated Hospital of Qingdao University, Qingdao, China

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