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ORIGINAL RESEARCH article

Front. Nutr.
Sec. Food Chemistry
Volume 11 - 2024 | doi: 10.3389/fnut.2024.1448908
This article is part of the Research Topic Bioactive Compounds from Medicinal Mushrooms and Plants - Extraction and Potential Application in Foods View all 3 articles

Isolation, Characterization and LC MS/MS Determination of Anti-obesity Components from Pine Needles of Cedrus deodara (Roxb.) G. Don

Provisionally accepted
Wang Xin Wang Xin 1Bin Li Bin Li 1Dongyan Liu Dongyan Liu 2*Yuer Guo Yuer Guo 3*Jiaxu Zhang Jiaxu Zhang 1*Wanyu Li Wanyu Li 1*Tengteng Peng Tengteng Peng 4*Quhuan Ma Quhuan Ma 2*Xiaofeng Shi Xiaofeng Shi 2*
  • 1 College of Pharmacy, Gansu University of Traditional Chinese Medicine, Lanzhou, China
  • 2 Gansu Academy of Sciences, Lanzhou, China
  • 3 Lanzhou University of Technology, Lanzhou, Gansu Province, China
  • 4 Northwest Normal University, Lanzhou, Gansu, China

The final, formatted version of the article will be published soon.

    Objective This study aimed to isolate and analyze the components in Cedar pine needles (needle leaves of Cedrus deodara (Roxb.) G. Don) that have a anti-obisity effect, guided by animal experiments. Methods The extract of Cedar pine needles was separated into four fractions of different polarities by a macroporous resin column. The fraction that retained the anti-obesity activity was evaluated based on the results of animal experiments. Monomeric compounds were isolated from the active fraction using a preparative liquid chromatography system combined with a glucose gel column and structurally characterized come with it. The content of the separated components was determined by UPLC-QQQ-MS/MS. Results The water-washed fraction retained the anti-obesity activity of Cedar pine needles better. A total of 16 compounds were separated from this fraction, and the contents of 14 of them were determined in Cedar pine needles. Conclusion Nine components including p-Hydroxybenzyl alcohol, Chlorogenic acid，Vanillic acid，Syringic acid，P-Coumaric acid，Sinapic acid，Benzoic acid， Phenylacetic acid， Sallcylic acid were characterized and determined for the first time in Cedar pine needles. The components with anti-obesity activity in the pine needles of the Cedrus may mainly come from phenolic acids.

    Keywords: Cedrus deodara (Roxb.) G. Don, Needle leaves, anti-obesity ingredients, phenolic acids, UPLC-QQQ-MS/MS

    Received: 14 Jun 2024; Accepted: 05 Jul 2024.

    Copyright: © 2024 Xin, Li, Liu, Guo, Zhang, Li, Peng, Ma and Shi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Dongyan Liu, Gansu Academy of Sciences, Lanzhou, China
    Yuer Guo, Lanzhou University of Technology, Lanzhou, Gansu Province, China
    Jiaxu Zhang, College of Pharmacy, Gansu University of Traditional Chinese Medicine, Lanzhou, China
    Wanyu Li, College of Pharmacy, Gansu University of Traditional Chinese Medicine, Lanzhou, China
    Tengteng Peng, Northwest Normal University, Lanzhou, 730070, Gansu, China
    Quhuan Ma, Gansu Academy of Sciences, Lanzhou, China
    Xiaofeng Shi, Gansu Academy of Sciences, Lanzhou, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.