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ORIGINAL RESEARCH article
Front. Nutr.
Sec. Nutrition and Microbes
Volume 11 - 2024 |
doi: 10.3389/fnut.2024.1442535
Glucosinolate extract from radish (Raphanus sativus L.) seed attenuates high-fat diet-induced obesity: Insights into gut microbiota and fecal metabolites
Provisionally accepted
Quanfeng Zhu
1*
Peng Zhang
1
Daqun Liu
2
Leilei Tang
1*
Jiawen Yu
1*
Chengcheng Zhang
2*
Guojun Jiang
1*- 1 Affiliated Hospital of Hangzhou Normal University, Hangzhou, China
- 2 Zhejiang Academy of Agricultural Sciences, Hangzhou, China
Background: Radish seed is a functional food with many beneficial health effects. Glucosinolates are characteristic components in radish seed that can be transformed into bioactive isothiocyanates by gut microbiota. Objective: The present study aims to assess anti-obesity efficacy of radish seed glucosinolates (RSGs) and explored the underlying mechanisms with a focus on gut microbiota and fecal metabolome. Methods: High-fat diet-induced obese mice were supplemented with different doses of RSGs extract for 8 weeks. Changes in body weight, serum lipid, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels; and pathological changes in the liver and adipose tissue were examined. Fecal metabolome and 16S rRNA gene sequencing were used to analyze alterations in fecal metabolite abundance and the gut microbiota, respectively. Results and Conclusion: Results showed that RSG extract prevented weight gain and decreased serum lipid, ALT, AST levels and lipid deposition in liver and epididymal adipocytes in obese mice. Treatment with RSG extract also increased gut microbiota diversity and altered the dominant bacteria genera in the gut microbiota, decreasing the abundance of Faecalibaculum and increasing the abundance of Allobaculum, Romboutsia, Turicibacter, and Akkermansia. Fecal metabolome results identified 570 differentially abundant metabolites, of which glucosinolate degradation products, such as sulforaphene and 7-methylsulfinylheptyl isothiocyanate, were significantly upregulated after RSG extract intervention. Furthermore, enrichment analysis of metabolic pathways showed that the anti-obesity effects of RSG extract may be mediated by alterations in bile secretion, fat digestion and absorption, and biosynthesis of plant secondary metabolites. Overall, RSG extract can inhibit the development of obesity, and the obesity-alleviating effects of RSG are related to alternative regulation of the gut microbiota and glucosinolate metabolites.
Keywords: Radish seeds, Glucosinolate, Obesity, Gut Microbiota, fecal metabolome
Received: 02 Jun 2024; Accepted: 22 Jul 2024.
Copyright: © 2024 Zhu, Zhang, Liu, Tang, Yu, Zhang and Jiang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Quanfeng Zhu, Affiliated Hospital of Hangzhou Normal University, Hangzhou, China
Leilei Tang, Affiliated Hospital of Hangzhou Normal University, Hangzhou, China
Jiawen Yu, Affiliated Hospital of Hangzhou Normal University, Hangzhou, China
Chengcheng Zhang, Zhejiang Academy of Agricultural Sciences, Hangzhou, China
Guojun Jiang, Affiliated Hospital of Hangzhou Normal University, Hangzhou, China
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