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ORIGINAL RESEARCH article

Front. Nutr.
Sec. Nutrition and Metabolism
Volume 11 - 2024 | doi: 10.3389/fnut.2024.1437373

NAFPD exacerbation by hyperlipidemia combined with hyperuricemia: a pilot rat experiment in lipidomics

Provisionally accepted
Jing-Yun Li Jing-Yun Li 1,2Yong-Jian Chen Yong-Jian Chen 2Shilin Li Shilin Li 2*Guorong Lyu Guorong Lyu 1,2Furong Yan Furong Yan 2Jiajing Guo Jiajing Guo 3Jing Cheng Jing Cheng 1Yun Chen Yun Chen 1Jiaojiao Lin Jiaojiao Lin 2Ya-Ting Zeng Ya-Ting Zeng 2
  • 1 Quanzhou Medical College, Quanzhou, Fujian Province, China
  • 2 The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian Province, China
  • 3 Other, Quanzhou, China

The final, formatted version of the article will be published soon.

    Background: Hyperuricemia and non-alcoholic fatty pancreas disease (NAFPD) are prevalent metabolic diseases, but the relationship between them remains underexplored.Methods: Eighteen Sprague-Dawley rats were randomly assigned to three groups: normal (CON), high-fat (PO), and high-fat high-uric acid (PH). After 12 weeks, serum uric acid (SUA) and triacylglycerol levels were measured. Pathological changes in the pancreas were assessed using hematoxylin-eosin (HE) staining. Serum samples were analyzed using lipidomics technology, and multivariate statistical analysis was employed to identify differences in lipid metabolism.Results: SUA levels in the PO group were not significantly different from those in the CON group (P>0.05). However, from the 4th week onward, SUA levels in the PH group were significantly higher than those in both the PO and CON groups (P<0.05). HE staining revealed that most rats in the CON group exhibited normal pancreatic islet and acinar cell morphology. The pathological NAFPD score in the PH group was higher than that in the PO group. Lipidomics analysis identified 34 potential serum biomarkers in the CON and PO groups, 38 in the CON and PH groups, and 32 in the PH and PO groups. These metabolites primarily included sphingolipids, cholesterol esters, fatty acids, triacylglycerols, phosphatidylcholines, lysophosphatidylcholine, phosphatidylethanolamine, and lysophosphatidylethanolamine.Hyperlipidemia combined with hyperuricemia might exacerbates NAFPD.Glycerophospholipids may serve as key biomarkers in this process, potentially linked to a chronic inflammatory response mediated by glycerophospholipids.

    Keywords: Hyperuricemia, lipidomics, Non-alcoholic fatty pancreas disease (NAFPD), Non-alcoholic fatty liver disease, Glycerophospholipids

    Received: 05 Oct 2024; Accepted: 18 Dec 2024.

    Copyright: © 2024 Li, Chen, Li, Lyu, Yan, Guo, Cheng, Chen, Lin and Zeng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Shilin Li, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, 362000, Fujian Province, China

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