Identification of ferroptosis-related genes signatures in temporal lobe epilepsy with hippocampal sclerosis

Fan Gao Jinzi Li ^*

• Department of Pediatrics, Yanbian University Hospital, Yanji, China

Background: Ferroptosis, a form of regulated cell death, contributes to the damage of central nervous system neuronsFerroptosis plays an important role on central nervous system neurons damage. Here, we aimed to construct a ferroptosis-related genes signatures in temporal lobe epilepsy with hippocampal sclerosis (TLE-HS) and explore their diagnostic role in TLE-HS.Methods: GSE205661 was downloaded as the training dataset, and GSE71058 was obtained for use as the validation dataset. Subsequently, ferroptosis-related differentially expressed genes (FR-DEGs) in TLE-HS were further analyzed.GSE205661 was downloaded as the training dataset and GSE71058 was downloaded as validation dataset, and then, ferroptosis-related differentially expressed genes (FR-DEGs) in TLE-HS were further analyzed. Weighed gene co-expression network analysis (WGCNA) algorithm, single factor logistic regression analysis, and LASSO algorithm were used to screen characteristic FR-DEGs. Then, receiver operating characteristic (ROC) was used to evaluate the value of these characteristic genes on disease diagnosis. Finally, long non-coding RNA (lncRNA)lncRNA-microRNA (miRNA)miRNA-mRNA network was constructed.We identified 141 FR-DEGs in TLE-HS, and these genes were enriched in T cell activation involved in immune response and signaling pathways related with lipid and atherosclerosis. Further WGCNA selected 47 overlapping FR-DEG, and they were significantly enriched in 13 biological processes and 14 KEGG pathways, including negative regulation of apoptotic process and ferroptosis. Four genes,