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BRIEF RESEARCH REPORT article
Front. Mol. Biosci.
Sec. Metabolomics
Volume 12 - 2025 | doi: 10.3389/fmolb.2025.1511119
Urinary marker of oxidative stress in children correlates with molecules in exhaled breath
Provisionally accepted- University Children’s Hospital Basel, Basel, Switzerland
Real-time breath analysis has shown potential as a non-invasive method for detecting oxidative stress and airway inflammation. However, there is a lack of data on the association of full breath profiles with established urinary biomarkers of oxidative stress and respiratory inflammation, which could help to advance the implementation of this method in the clinical practice. We analyzed breath profiles of 25 tobacco smoke-exposed and 103 non-exposed children using real-time secondary electrospray ionization high-resolution mass spectrometry (SESI-HRMS) and determined in parallel urinary concentrations of biomarkers of oxidative stress and respiratory inflammation. We evaluated the correlation between breath features and urinary biomarkers and tested the prediction of these biomarkers by exhaled breath. We found 71 breath features that correlated significantly with the urinary oxidative stress marker 8-iso-prostaglandin F2α (8-iso-PGF2α). The agreement (mean (standard deviation) Lin's concordance correlation) between breath-predicted and actual urinary 8-iso-PGF2α levels was 0.37 (0.05). In conclusion, our results suggest that the real-time breath analysis with SESI-HRMS has promising potential to gauge oxidative stress.
Keywords: Urine, Oxidative Stress, exhaled breath, Child, Smoking
Received: 14 Oct 2024; Accepted: 26 Feb 2025.
Copyright: © 2025 Gisler, Singh, Marten, Decrue, Frey, Sinues and Usemann. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jakob Usemann, University Children’s Hospital Basel, Basel, Switzerland
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