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ORIGINAL RESEARCH article

Front. Mol. Biosci.
Sec. Molecular Diagnostics and Therapeutics
Volume 11 - 2024 | doi: 10.3389/fmolb.2024.1494779

PLASMA PROTEOME FINGERPRINT IN KIDNEY DISEASES

Provisionally accepted
Kirill S. Nikolsky Kirill S. Nikolsky 1Arthur T. Kopylov Arthur T. Kopylov 1Valeriya I. Nakhod Valeriya I. Nakhod 1Natalia V. Potoldykova Natalia V. Potoldykova 2Dmitry V. Enikeev Dmitry V. Enikeev 2Tatiana V. Butkova Tatiana V. Butkova 1Liudmila I. Kulikova Liudmila I. Kulikova 1Kristina A. Malsagova Kristina A. Malsagova 1Vladimir R. Rudnev Vladimir R. Rudnev 1Denis V. Petrovsky Denis V. Petrovsky 1Alexander A. Izotov Alexander A. Izotov 1Anna L. Kaysheva Anna L. Kaysheva 1*
  • 1 Institute of Biomedical Chemistry, Moscow, Russia
  • 2 Institute for Urology and Reproductive Health, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia

The final, formatted version of the article will be published soon.

    Kidney diseases pose a serious healthcare problem because of their high prevalence, worsening of patients' quality of life, and high mortality. Patients with kidney diseases are often asymptomatic until disease progression starts. Expensive renal replacement therapy options, such as dialysis or kidney transplant, are required for end-stage kidney disease. Early diagnosis of kidney pathology is crucial for slowing down or curbing further damage.This study aimed to analyze the features of the protein composition of blood plasma in patients with the most common kidney pathologies: kidney calculus, kidney cyst, and kidney cancer. The study involved 75 subjects. Proteins associated with kidney pathologies (CFB, SERPINA3, HPX, HRG, SERPING1, HBB, ORM2, and CP) were proposed. These proteins are important participants of complement and coagulation cascade activation and lipid metabolism. The revealed phosphorylated proteoforms (CFB, C4A/C4B, F2, APOB, TTR, and NRAP) were identified. For them, modification sites were mapped on 3D protein models, and the potential role in formation of complexes with native partner proteins was assessed. The study demonstrates that the selected kidney pathologies have a similar proteomic profile, and patients can be classified into kidney pathology groups with an accuracy of (70-80)%.

    Keywords: Kidney Diseases, Kidney calculus, Kidney cyst, Cancer, Tandem Mass Spectrometry, Protein Analysis, Phosphorylation, Candidate markers

    Received: 11 Sep 2024; Accepted: 10 Dec 2024.

    Copyright: © 2024 Nikolsky, Kopylov, Nakhod, Potoldykova, Enikeev, Butkova, Kulikova, Malsagova, Rudnev, Petrovsky, Izotov and Kaysheva. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Anna L. Kaysheva, Institute of Biomedical Chemistry, Moscow, Russia

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.