Amany Hamed ^1,2* Fatma Abo Zakaib Ali ³ Ard Elshifa M E Mohammed ⁴ Muneera Alrasheedi ⁴ Islam Ragab ⁴ Maryam Aldoghaim ⁵ Safaa S Soliman ⁶

• ¹ Sohag University, Sohag, Egypt
• ² Faculty of Science, Sohag University, Sohag, Egypt
• ³ Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt
• ⁴ Department of chemisttry, College of Science at Unaizah, Qassim University, unaizah, Saudi Arabia
• ⁵ Department of Chemistry, College of Science, King Faisal University, Al-Ahsa, Eastern Province, Saudi Arabia
• ⁶ Minia University, Minya, Minya, Egypt

Methidathion [1] is commonly used in agriculture and has adverse effects on reproduction. Chrysin [2] has several advantageous properties, such as anti-inflammatory, anti-cancer, and antioxidant properties. The purpose of the current investigation was to assess CHR's therapeutic efficacy in reducing ovarian toxicity brought on by MD. Twenty-four female rats were divided into four groups of six animals each. Group 1 served as a control, while group 2 rats received MD (5 mg/kg). Rats in Group 3 received CHR at a dose of 50 mg/kg. Rats in group 4 received treatment with CHR after MD intoxication. Our research revealed that MD significantly (p < 0.001) increased the levels of MDA, caspase-3, FSH, LH, CA-125, and TNF-α but significantly (p < 0.001) decreased the levels of SOD, GSH, E2, and progesterone when compared to the control and CHR groups. After receiving CHR therapy, damage induced by MD was significantly (p < 0.001) repaired. In summary, this study showed that CHR could mitigate the adverse effects that MD causes to the ovaries by decreasing oxidative stress, inflammation, and apoptosis; improving antioxidant status; and restoring the correct ratio of sex hormones.