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ORIGINAL RESEARCH article

Front. Med.
Sec. Pulmonary Medicine
Volume 11 - 2024 | doi: 10.3389/fmed.2024.1473080
This article is part of the Research Topic Advancements in Multimodal Data Analysis for Lung Tumor Diagnosis View all 6 articles

Investigating the role of disulfidptosis related genes in radiotherapy resistance of lung adenocarcinoma

Provisionally accepted
Gaoren Wang Gaoren Wang 1,2*Xiaoxia Pan Xiaoxia Pan 1,2Hongyan Qian Hongyan Qian 1Zhouna Sun Zhouna Sun 1Qiong Yi Qiong Yi 1Ying Liu Ying Liu 1Languangzhi Languangzhi Languangzhi Languangzhi 1,2Chen Jia Chen Jia 1
  • 1 Nantong Tumor Hospital, Nantong, China
  • 2 School of Medicine, Nantong University, Nantong, Jiangsu Province, China

The final, formatted version of the article will be published soon.

    Background: Radiotherapy resistance is an important reason for high mortality in lung cancer patients, but the mechanism is still unclear. Dysregulation of cell proliferation and death plays a crucial role in the onset and progression of lung adenocarcinoma (LUAD). In recent times, a novel form of cellular demise called disulfidoptosis, has attracted increasing attention. However, it is unclear whether the radiation-related disulfidptosis genes have prognostic role in LUAD.Methods: A complete suite of bioinformatics tools was used to analyze the expression and prognostic significance of radiation-related disulfidptosis genes. Afterwards, we investigated the predictive significance of the risk signature in tumour microenvironments (TME), somatic mutations, and immunotherapies. In addition, we conducted a series of experiments to verify the expression of differentially expressed radiotherapy related disulfidptosis genes (DERRDGs) in vitro.Results: A total of 88 DERRDGs were found. We constructed and validated a novel prognostic model based on PRELP, FGFBP1, CIITA and COL5A1. The enrichment analysis showed the DERRDG affected tumor prognosis by influencing tumor microenvironments (TME) and immunotherapy. And we constructed nomogram to promote clinical application. In addition, q-PCR confirmed the significant differences in the expression of prognostic genes between A549 irradiation-resistance cell and A549. Finally, western-blot, IHC staining, and small interference experiment suggested that PRELP may be a potential biomarker for radiotherapy resistance, whose low expression was associated with poor outcomes in LUAD patients.This study reveals the signature and possible underlying mechanisms of DERRDGs in LUAD and discovered the key gene PRELP, which helps to identify new prognostic biomarkers and provides a basis for future research.

    Keywords: LUAD, Radiotherapy, disulfidptosis, prognosis, Tumor immune microenvironment

    Received: 30 Jul 2024; Accepted: 10 Oct 2024.

    Copyright: © 2024 Wang, Pan, Qian, Sun, Yi, Liu, Languangzhi and Jia. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Gaoren Wang, Nantong Tumor Hospital, Nantong, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.