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REVIEW article

Front. Mech. Eng.
Sec. Biomechanical Engineering
Volume 10 - 2024 | doi: 10.3389/fmech.2024.1481933
This article is part of the Research Topic Advancements in Multiscale Characterization and Modeling of Cardiovascular Tissues View all articles

Bridging High Resolution Sub-Cellular Imaging with Physiologically Relevant Engineered Tissues

Provisionally accepted
Yasaman Kargar Gaz Kooh Yasaman Kargar Gaz Kooh Nathaniel Huebsch Nathaniel Huebsch *
  • Washington University in St. Louis, St. Louis, United States

The final, formatted version of the article will be published soon.

    While high-resolution microscopic techniques are crucial for studying cellular structures in cell biology, obtaining such images from thick 3D engineered tissues remains challenging. In this review, we explore advancements in fluorescence microscopy, alongside the use of various fluorescent probes and material processing techniques to address these challenges. We navigate through the diverse array of imaging options available in tissue engineering field, from wide field to super-resolution microscopy, so researchers can make more informed decisions based on the specific tissue and cellular structures of interest. Finally, we provide some recent examples of how traditional limitations on obtaining high-resolution images on sub-cellular architecture within 3D tissues have been overcome by combining imaging advancements with innovative tissue engineering approaches.

    Keywords: Fluorescence Microscopy1, High-Resolution Imaging2, tissue engineering3, Photo-physics4, Sub-cellular Resolution

    Received: 16 Aug 2024; Accepted: 31 Oct 2024.

    Copyright: © 2024 Kargar Gaz Kooh and Huebsch. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Nathaniel Huebsch, Washington University in St. Louis, St. Louis, United States

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.