Single-Cell Sequencing Elucidates the Mechanism of NUSAP1 in Glioma and Its Diagnostic and Prognostic Significance

Mengyu Zhao Zhao-Lei Shen Hongzhen Dai Wan-Yan Xu Lina Wang Tian-Hang Yu Cun-Zhi Wang JiaFeng Xu Guan-Jun Chen Dong-Hui Chen Wenming Hong ^* Fang Zhang

• First Affiliated Hospital of Anhui Medical University, Hefei, China

Background: Personalized precision medicine (PPPM) in cancer immunology and oncology is a rapi dly advancing field with significant potential. Gliomas, known for their poor prognosis, rank among t he most lethal brain tumors. Despite advancements, there remains a critical need for precise, individu alized treatment strategies.We conducted a comprehensive analysis of RNA-seq and microarray data from the TCGA and GEO databases, supplemented by single-cell RNA sequencing (scRNA-seq) data from glioma pat ients. By integrating single-cell sequencing analysis with foundational experiments, we investigated t he molecular variations and cellular interactions within neural glioma cell subpopulations during tum or progression.Results: Our single-cell sequencing analysis revealed distinct gene expression patterns across glioma cell subpopulations. Notably, differentiation trajectory analysis identified NUSAP1 as a key marker f or the terminal subpopulation. We found that elevated NUSAP1 expression correlated with poor prog nosis, prompting further investigation of its functional role through both cellular and animal studies.. Conclusions: NUSAP1-based risk models hold potential as predictive and therapeutic tools for perso nalized glioma treatment. In-depth exploration of NUSAP1's mechanisms in glioblastoma could enha nce our understanding of its response to immunotherapy, suggesting that targeting NUSAP1 may offe r therapeutic benefits for glioma patients.