Skip to main content

ORIGINAL RESEARCH article

Front. Immunol.
Sec. Cancer Immunity and Immunotherapy
Volume 15 - 2024 | doi: 10.3389/fimmu.2024.1505966
This article is part of the Research Topic Community Series in Novel Preclinical Model, Biomarker, Treatment and Drug Delivery to Address Immune Evasion in Cancer: Volume II View all articles

The Close Association of Muribaculum and PA (10:0/a-17:0) with the Occurrence of Pancreatic Ductal Adenocarcinoma and Immunotherapy

Provisionally accepted
Enzhao Wang Enzhao Wang 1Kuiwu Ren Kuiwu Ren 1Xiangyu Wang Xiangyu Wang 1Sen Du Sen Du 1Xiang Gao Xiang Gao 2Wang Niu Wang Niu 1Chenyue Guan Chenyue Guan 1Xue Liu Xue Liu 1Panpan Wu Panpan Wu 1Chunlong Liu Chunlong Liu 2Jiangtao Yu Jiangtao Yu 2*Kun Song Kun Song 3*
  • 1 Department of Hepatobiliary and Pancreatic Surgery, Fuyang People’s Hospital, Bengbu Medical College, Fuyang, Jiangsu Province, China
  • 2 Department of Hepatobiliary and Pancreatic Surgery, Fuyang People’s Hospital, Anhui Medical University, Fuyang, Jiangsu Province, China
  • 3 Fuyang People’s Hospital, Fuyang City, China

The final, formatted version of the article will be published soon.

    Background: Progress in immunotherapy for pancreatic ductal adenocarcinoma (PDAC) has been slow, yet the relationship between microorganisms and metabolites is crucial to PDAC development. This study compares the biliary microbiota and metabolomic profiles of PDAC patients with those of benign pancreatic disease patients to investigate PDAC pathogenesis and its relationship with immunotherapy.Methods: A total of 27 patients were recruited, including 15 diagnosed with PDAC and 12 with benign pancreaticobiliary conditions, all of whom underwent surgical treatment. Intraoperative bile samples were collected and analyzed using 16S rRNA sequencing in conjunction with liquid chromatographymass spectrometry (LC-MS). Multivariate statistical methods and correlation analyzes were employed to assess differences in microbial composition, structure, and function between malignant and benign pancreatic diseases. Additionally, a retrospective analysis was conducted on PDAC patients postsurgery regarding immunotherapy and its correlation with metabolic components.Results: PDAC patients exhibited a significantly higher abundance of bile microbiota compared to controls, with notable differences in microbiota structure between the two groups (P < 0.05). At the genus level, Muribaculum was markedly enriched in the bile of PDAC patients and was strongly correlated with phosphatidic acid (PA) (10:0/a-17:0). Both of these components, along with the tumor marker CA199, formulated a predictor of PDAC. Furthermore, PA (10:0/a-17:0) demonstrated a strong correlation with PDAC immunotherapy outcomes (Rho: 0.758; P=0.011).These findings suggest that the biliary microbiota and associated metabolites play a crucial role in the development of PDAC and may serve as potential predictive biomarkers and therapeutic targets for disease management.

    Keywords: 16S rRNA sequencing, Metabolomics, Bile, PDAC, Immunotherapy

    Received: 04 Oct 2024; Accepted: 06 Nov 2024.

    Copyright: © 2024 Wang, Ren, Wang, Du, Gao, Niu, Guan, Liu, Wu, Liu, Yu and Song. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Jiangtao Yu, Department of Hepatobiliary and Pancreatic Surgery, Fuyang People’s Hospital, Anhui Medical University, Fuyang, Jiangsu Province, China
    Kun Song, Fuyang People’s Hospital, Fuyang City, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.