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ORIGINAL RESEARCH article
Front. Immunol.
Sec. Microbial Immunology
Volume 15 - 2024 |
doi: 10.3389/fimmu.2024.1427510
This article is part of the Research Topic Immune Mechanisms of Protection Against Mycobacterium tuberculosis View all 6 articles
The Chosen Few: Mycobacterium tuberculosis isolates for IMPAc-TB
Provisionally accepted- 1 Seattle Children's Research Institute, Seattle, United States
- 2 Department of Genetics, School of Medicine, University of North Carolina at Chapel Hill, Chapell hill, North Carolina, United States
- 3 Department of Immunology and Infectious Diseases, School of Public Health, Harvard University, Boston, Massachusetts, United States
- 4 Broad Institute, Cambridge, MA, United States
- 5 Department of Immunology, School of Medicine, University of Washington, Seattle, Washington, United States
- 6 Department of Pediatrics, School of Medicine, University of Washington, Seattle, Washington, United States
- 7 Department of Global Health, School of Public Health, University of Washington, Seattle, Washington, United States
The three programs that make up the Immune Mechanisms of Protection Against Mycobacterium tuberculosis Centers (IMPAc-TB) had to prioritize and select strains to be leveraged for this work. The CASCADE team based at Seattle Children’s Research Institute are leveraging M.tb H37Rv, M.tb CDC1551, and M.tb SA161. The HI-IMPACT team based at Harvard T.H. Chan School of Public Health, Boston have selected M.tb Erdman as well as a novel clinical isolate recently characterized during a longitudinal study in Peru. The PHOENIX team also based at Seattle Children’s Research Institute, have selected M.tb HN878 and M.tb Erdman as their isolates of choice. Here we describe original source isolation, genomic references, key virulence characteristics, and relevant tools that make these isolates attractive for use. The global context for M.tb lineage 2 and 4 selection is reviewed including what is known about their relative abundance and acquisition of drug resistance. Host-pathogen interactions seem driven by genomic differences on each side and these play an important role in pathogenesis and immunity. The few M.tb strains chosen for this work do not reflect the vast genomic diversity within this species. They do, however, provide specific virulence, pathology and growth kinetics of interest to the consortium. The strains selected should not be considered as “representative” of the growing available array of M.tb isolates, but rather tools that are being used to address key outstanding questions in the field.
Keywords: Mycobacterium tuberculosis, Tuberculosis, TB, Lineages, diversity, isolates, IMPAc-TB
Received: 03 May 2024; Accepted: 06 Sep 2024.
Copyright: © 2024 Larsen, Abdelaal, Plumlee, Cohen, Kim, Liu, Harband, Berube, Baldwin, Fortune, Urdahl and Coler. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Sasha E. Larsen, Seattle Children's Research Institute, Seattle, United States
Rhea N. Coler, Seattle Children's Research Institute, Seattle, United States
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