Corrigendum: Re-expression of tafazzin isoforms in TAZ-deficient C6 glioma cells restores cardiolipin composition but not proliferation rate and alterations in gene expression

A Corrigendum on
Re-expression of tafazzin isoforms in TAZ-deficient C6 glioma cells restores cardiolipin composition but not proliferation rate and alterations in gene expression

by Jagirdar G, Elsner M, Scharf C, Simm S, Borucki K, Peter D, Lalk M, Methling K, Linnebacher M, Krohn M, Wolke C and Lendeckel U (2022). Front. Genet. 13:931017. doi: 10.3389/fgene.2022.931017

In the published article, there was an error in “Figures 1–5 and Table 1” as published. The figures were not in correct order and some human primers shown in Table 1 were not used in the study. The corrected figures and tables appear below.

FIGURE 1

FIGURE 1. Tafazzin deficiency alters oxylipin levels in C6 cells: Effect of tafazzin knockout on cellular lipids, oxylipins was determined by HPLC-MS/MS. The Data analysis was performed with Agilent Mass Hunter Qualitative and Quantitative Analysis software (version B.08.00 for both). For all detected oxylipins, a relative quantification was done by normalizing the area of the metabolite signal to the area of the signal of the internal standard compound (relative amount). Data is represented as median,quartile and interquartile range for n = 4.

FIGURE 2

FIGURE 2. Tafazzin deficiency alters gene expression in C6 cells: Quantification levels of indicated genes in C6 and C6 TAZ cells was preformed and the amount of mRNA was determined by using RT-qPCR and normalized to RPL13a. Data is given as boxplot with median, quartile, and interquartile range (*p < 0.05; **p < 0.01; ***p < 0.001). Outliers are indicated using Tukey method. n = 8.

FIGURE 3

FIGURE 3. Oxylipins regulates TAZ dependent gene expression in C6 cells: Relative gene expression of C6 cells treated with ethanol (control) and C6 cells with 3 μM EET incubated at 37°C for 24 h. The amount of Mrna is determined by qpcr that is normalized to RPL13a. Data is represented as boxplot with median, quartile and interquartile range. Asterisks represent significant change.

FIGURE 4

FIGURE 4. IPA Upstream Regulators Analysis for identification of upstream regulators that may be responsible for changes in gene expression observed for ACACA, HMGCR, ACSS2, FASN, ACSL 1,3 and 4 (predicted activation (orange) predicted inhibition (blue) of the regulators leads to a decreased expression (green)).

FIGURE 5

FIGURE 5. (A) Left: Microscopic images of β-gal positive cells (blue color) in C6 and C6 TAZ cells. Right: Percentage of β-gal positive cells is given as boxplot (* indicates p < 0.05). (B) Upregulation of SASP genes: Amounts of mrnas of CXCL1, IL6, and IL1α are elevated in C6 TAZ cells (mrna expression normalized to RPL13a; n = 4, p = 0.029).

TABLE 1

TABLE 1. List of primers used in the study.

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