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ORIGINAL RESEARCH article
Front. Chem.
Sec. Electrochemistry
Volume 12 - 2024 |
doi: 10.3389/fchem.2024.1490940
Amino-functionalized vertically-ordered mesoporous silica film on electrochemically polarized screen-printed carbon electrode for the construction of gated electrochemical aptasensor and sensitive detection of carcinoembryonic antigen
Provisionally accepted- 1 Guangxi Medical University, Nanning, Guangxi Zhuang Region, China
- 2 Zhejiang Sci-Tech University, Hangzhou, China
Disposable electrochemical biosensors with high sensitivity are very fit for point-of-care testing in clinical diagnosis. Herein, amino-functionalized vertically-ordered mesoporous silica films (NH2-VMSF) attached to the electrochemically polarized screen-printed carbon electrode (p-SPCE) are prepared using a simple electrochemical method and then utilized to construct gated electrochemical aptasensor for rapid and sensitive determination of carcinoembryonic antigen (CEA). After being treated with the electrochemical polarization procedure, p-SPCE has plentiful oxygen-containing groups and improved catalytic ability, which is helpful for promoted stability of NH2-VMSF on SPCE without the use of adhesive layer and simultaneously generates highly electro-active sensing interface. Owing to the numerous uniform and ultrasmall nanopores of NH2-VMSF, CEA-specific aptamer anchored on the external surface of NH2-VMSF/p-SPCE serves as the gatekeeper, allowing the specific recognition and binding of CEA and eventually impeding the ingress of electrochemical probes (Fe(CN)6 3-/4-) through the silica nanochannels. The declined electrochemical responses of Fe(CN)6 3-/4- can be used to quantitatively detect CEA, yielding a wide detection range (100 fg/mL to 100 ng/mL) and a low limit of detection (24 fg/mL). Moreover, the proposed NH2-VMSF/p-SPCE based electrochemical aptasensor can be applied to detect the amount of CEA in spiked human serum samples, which extends the biological application of disposable NH2-VMSF/p-SPCE sensor by modulating the biological recognition species.
Keywords: Amino-functionalized vertically-ordered mesoporous silica film, Screen-Printed Carbon Electrode, Electrochemical polarization, Carcinoembryonic Antigen, Electrochemical aptasensor
Received: 04 Sep 2024; Accepted: 14 Oct 2024.
Copyright: © 2024 He, Wang, Luo, Yan and Guo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Ke He, Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Region, China
Tao Luo, Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Region, China
Fei Yan, Zhejiang Sci-Tech University, Hangzhou, China
Jing Guo, Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Region, China
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